Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats

Autores
Araujo, Alex Sander Da Rosa; Miranda, Madalena Freitas Silva de; Oliveira, Ubirajara de; Fernandes, Tânia; Llesuy, Susana Francisca; Kucharski, Luiz Carlos Rios; Khaper, Neelam; Belló Klein, Adriane
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this study was to determine whether decreased oxidative stress would increase the resistance to cardiac contracture induced by H 2O2 in hypothyroid rats. Male Wistar rats were divided into two groups: control and hypothyroid. Hypothyroidism was induced via thyroidectomy. Four weeks post surgery, blood samples were collected to perform thyroid hormone assessments, and excised hearts were perfused at a constant flow with or without H2O2 (1 mmol/L), being divided into two sub-groups: control, hypothyroid, control + H2O2, hypothyroid + H2O2. Lipid peroxidation (LPO) was evaluated by chemiluminescence (CL) and thiobarbituric acid reactive substances (TBARS) methods, and protein oxidation by carbonyls assay in heart homogenates. Cardiac tissue was also screened for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, and for total radical-trapping antioxidant potential (TRAP). Analyses of SOD and glutathione-S-transferase (GST) protein expression were also performed in heart homogenates. Hypothyroid hearts were found to be more resistant to H2O2-induced contracture (60% elevation in LVEDP) as compared to control. CL, TBARS, carbonyl, as well as SOD, CAT, GPx activities and TRAP levels were reduced (35, 30, 40, 30, 16, 25, and 33%, respectively) in the cardiac homogenates of the hypothyroid group as compared to controls. A decrease in SOD and GST protein levels by 20 and 16%, respectively, was also observed in the hypothyroid group. These results suggest that a hypometabolic state caused by thyroid hormone deficiency can lead to an improved response to H2O2 challenge and is associated with decreased oxidative myocardial damage.
Fil: Araujo, Alex Sander Da Rosa. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Miranda, Madalena Freitas Silva de. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oliveira, Ubirajara de. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, Tânia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Kucharski, Luiz Carlos Rios. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Khaper, Neelam. Lakehead University; Canadá
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
Materia
Antioxidant Enzymes
Heart Function
Oxidative Damage
Protein Expression
Thyroid Hormones
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67677
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67677
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid ratsAraujo, Alex Sander Da RosaMiranda, Madalena Freitas Silva deOliveira, Ubirajara deFernandes, TâniaLlesuy, Susana FranciscaKucharski, Luiz Carlos RiosKhaper, NeelamBelló Klein, AdrianeAntioxidant EnzymesHeart FunctionOxidative DamageProtein ExpressionThyroid Hormoneshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The purpose of this study was to determine whether decreased oxidative stress would increase the resistance to cardiac contracture induced by H 2O2 in hypothyroid rats. Male Wistar rats were divided into two groups: control and hypothyroid. Hypothyroidism was induced via thyroidectomy. Four weeks post surgery, blood samples were collected to perform thyroid hormone assessments, and excised hearts were perfused at a constant flow with or without H2O2 (1 mmol/L), being divided into two sub-groups: control, hypothyroid, control + H2O2, hypothyroid + H2O2. Lipid peroxidation (LPO) was evaluated by chemiluminescence (CL) and thiobarbituric acid reactive substances (TBARS) methods, and protein oxidation by carbonyls assay in heart homogenates. Cardiac tissue was also screened for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, and for total radical-trapping antioxidant potential (TRAP). Analyses of SOD and glutathione-S-transferase (GST) protein expression were also performed in heart homogenates. Hypothyroid hearts were found to be more resistant to H2O2-induced contracture (60% elevation in LVEDP) as compared to control. CL, TBARS, carbonyl, as well as SOD, CAT, GPx activities and TRAP levels were reduced (35, 30, 40, 30, 16, 25, and 33%, respectively) in the cardiac homogenates of the hypothyroid group as compared to controls. A decrease in SOD and GST protein levels by 20 and 16%, respectively, was also observed in the hypothyroid group. These results suggest that a hypometabolic state caused by thyroid hormone deficiency can lead to an improved response to H2O2 challenge and is associated with decreased oxidative myocardial damage.Fil: Araujo, Alex Sander Da Rosa. Universidade Federal do Rio Grande do Sul; BrasilFil: Miranda, Madalena Freitas Silva de. Universidade Federal do Rio Grande do Sul; BrasilFil: Oliveira, Ubirajara de. Universidade Federal do Rio Grande do Sul; BrasilFil: Fernandes, Tânia. Universidade Federal do Rio Grande do Sul; BrasilFil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Kucharski, Luiz Carlos Rios. Universidade Federal do Rio Grande do Sul; BrasilFil: Khaper, Neelam. Lakehead University; CanadáFil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; BrasilJohn Wiley & Sons Ltd2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67677Araujo, Alex Sander Da Rosa; Miranda, Madalena Freitas Silva de; Oliveira, Ubirajara de; Fernandes, Tânia; Llesuy, Susana Francisca; et al.; Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats; John Wiley & Sons Ltd; Cell Biochemistry And Function; 28; 1; 1-2010; 38-440263-6484CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/cbf.1616info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/cbf.1616info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:15Zoai:ri.conicet.gov.ar:11336/67677instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:15.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
title Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
spellingShingle Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
Araujo, Alex Sander Da Rosa
Antioxidant Enzymes
Heart Function
Oxidative Damage
Protein Expression
Thyroid Hormones
title_short Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
title_full Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
title_fullStr Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
title_full_unstemmed Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
title_sort Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats
dc.creator.none.fl_str_mv Araujo, Alex Sander Da Rosa
Miranda, Madalena Freitas Silva de
Oliveira, Ubirajara de
Fernandes, Tânia
Llesuy, Susana Francisca
Kucharski, Luiz Carlos Rios
Khaper, Neelam
Belló Klein, Adriane
author Araujo, Alex Sander Da Rosa
author_facet Araujo, Alex Sander Da Rosa
Miranda, Madalena Freitas Silva de
Oliveira, Ubirajara de
Fernandes, Tânia
Llesuy, Susana Francisca
Kucharski, Luiz Carlos Rios
Khaper, Neelam
Belló Klein, Adriane
author_role author
author2 Miranda, Madalena Freitas Silva de
Oliveira, Ubirajara de
Fernandes, Tânia
Llesuy, Susana Francisca
Kucharski, Luiz Carlos Rios
Khaper, Neelam
Belló Klein, Adriane
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antioxidant Enzymes
Heart Function
Oxidative Damage
Protein Expression
Thyroid Hormones
topic Antioxidant Enzymes
Heart Function
Oxidative Damage
Protein Expression
Thyroid Hormones
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The purpose of this study was to determine whether decreased oxidative stress would increase the resistance to cardiac contracture induced by H 2O2 in hypothyroid rats. Male Wistar rats were divided into two groups: control and hypothyroid. Hypothyroidism was induced via thyroidectomy. Four weeks post surgery, blood samples were collected to perform thyroid hormone assessments, and excised hearts were perfused at a constant flow with or without H2O2 (1 mmol/L), being divided into two sub-groups: control, hypothyroid, control + H2O2, hypothyroid + H2O2. Lipid peroxidation (LPO) was evaluated by chemiluminescence (CL) and thiobarbituric acid reactive substances (TBARS) methods, and protein oxidation by carbonyls assay in heart homogenates. Cardiac tissue was also screened for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, and for total radical-trapping antioxidant potential (TRAP). Analyses of SOD and glutathione-S-transferase (GST) protein expression were also performed in heart homogenates. Hypothyroid hearts were found to be more resistant to H2O2-induced contracture (60% elevation in LVEDP) as compared to control. CL, TBARS, carbonyl, as well as SOD, CAT, GPx activities and TRAP levels were reduced (35, 30, 40, 30, 16, 25, and 33%, respectively) in the cardiac homogenates of the hypothyroid group as compared to controls. A decrease in SOD and GST protein levels by 20 and 16%, respectively, was also observed in the hypothyroid group. These results suggest that a hypometabolic state caused by thyroid hormone deficiency can lead to an improved response to H2O2 challenge and is associated with decreased oxidative myocardial damage.
Fil: Araujo, Alex Sander Da Rosa. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Miranda, Madalena Freitas Silva de. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oliveira, Ubirajara de. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, Tânia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Kucharski, Luiz Carlos Rios. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Khaper, Neelam. Lakehead University; Canadá
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
description The purpose of this study was to determine whether decreased oxidative stress would increase the resistance to cardiac contracture induced by H 2O2 in hypothyroid rats. Male Wistar rats were divided into two groups: control and hypothyroid. Hypothyroidism was induced via thyroidectomy. Four weeks post surgery, blood samples were collected to perform thyroid hormone assessments, and excised hearts were perfused at a constant flow with or without H2O2 (1 mmol/L), being divided into two sub-groups: control, hypothyroid, control + H2O2, hypothyroid + H2O2. Lipid peroxidation (LPO) was evaluated by chemiluminescence (CL) and thiobarbituric acid reactive substances (TBARS) methods, and protein oxidation by carbonyls assay in heart homogenates. Cardiac tissue was also screened for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, and for total radical-trapping antioxidant potential (TRAP). Analyses of SOD and glutathione-S-transferase (GST) protein expression were also performed in heart homogenates. Hypothyroid hearts were found to be more resistant to H2O2-induced contracture (60% elevation in LVEDP) as compared to control. CL, TBARS, carbonyl, as well as SOD, CAT, GPx activities and TRAP levels were reduced (35, 30, 40, 30, 16, 25, and 33%, respectively) in the cardiac homogenates of the hypothyroid group as compared to controls. A decrease in SOD and GST protein levels by 20 and 16%, respectively, was also observed in the hypothyroid group. These results suggest that a hypometabolic state caused by thyroid hormone deficiency can lead to an improved response to H2O2 challenge and is associated with decreased oxidative myocardial damage.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67677
Araujo, Alex Sander Da Rosa; Miranda, Madalena Freitas Silva de; Oliveira, Ubirajara de; Fernandes, Tânia; Llesuy, Susana Francisca; et al.; Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats; John Wiley & Sons Ltd; Cell Biochemistry And Function; 28; 1; 1-2010; 38-44
0263-6484
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67677
identifier_str_mv Araujo, Alex Sander Da Rosa; Miranda, Madalena Freitas Silva de; Oliveira, Ubirajara de; Fernandes, Tânia; Llesuy, Susana Francisca; et al.; Increased resistance to hydrogen peroxide-induced cardiac contracture is associated with decreased myocardial oxidative stress in hypothyroid rats; John Wiley & Sons Ltd; Cell Biochemistry And Function; 28; 1; 1-2010; 38-44
0263-6484
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/cbf.1616
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/cbf.1616
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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