Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes

Autores
Aguero, María Belen; Svetaz, Laura Andrea; Baroni, María Verónica; Lima, Beatriz Viviana; Luna, Lorena Celina; Zacchino, Susana Alicia Stella; Saavedra, Pedro; Wunderlin, Daniel Alberto; Feresin, Gabriela Egly; Tapia, Aníbal Alejandro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Propolis is widely used in the folk medicine of San Juan province (Argentina) to treat several diseases, including cold, cough, muscle aches and superficial mycoses. We report the in vitro antifungal activity of urban propolis, evaluated with CLSI protocols in addition to the evaluation of their chemical profile by HPLC-ESI-MS/MS techniques. The dermatophytes Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum were the most susceptible species and guided the fractionation of urban propolis, which was performed with Sephadex LH-20 leading to eight fractions (I–VIII). These fractions showed high antifungal activities against dermatophytes (MICs = 16.0–62.5 μg/mL) and yeasts (MICs = 31.2–125 μg/mL) being III, V and VI the most active ones (MIC100 = 16–31.2 μg/mL). They also, showed fungicidal capacity (a condition highly appreciated in antifungal drugs to avoid recurrence) with MFC values between 31.2 and 62.5 μg/mL. From the most active fractions, two lignans: 3′-methyl-nordihydroguaiaretic acid (MNDGA) (1), and nordihydroguaiaretic acid (NDGA) (2), in addition to three flavonoids: chrysin (3), pinocembrin (4) and galangin (5), were isolated and quantified by HPLC–PDA-MS/MS as the main antifungal compounds. Lignans 1 and 2 showed strong activities against T. mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 31.2 and 62.5 μg/mL), and 1 showed strong activity against Candida albicans, Candida tropicalis and Cryptococcus neoformans (MICs between 31.2 and 62.5 μg/mL). Regarding flavonoids, all yeasts were sensitive to 5 (MIC = 31.2 μg/mL), whereas the dermatophytes T. mentagrophytes and T. rubrum and all yeasts were moderately inhibited by 4 (MIC = 31.2–250 μg/mL). Finally, chrysin (3) showed low activity against yeasts and dermatophytes (MIC = 250 μg/mL). These results support that Argentinean urban propolis, which are frequently used by beekeepers for the preparation of syrups, tinctures and creams, are valuable natural product for the improving of human health, particularly fungal infections. It is also worthy to take into account that its chemical composition contains mainly two antifungal lignans, associated with the medicinal Larrea genus
Fil: Aguero, María Belen. Universidad Nacional de San Juan; Argentina
Fil: Svetaz, Laura Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Baroni, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luna, Lorena Celina. Universidad Nacional de San Juan; Argentina
Fil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saavedra, Pedro. APICENTRO; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tapia, Aníbal Alejandro. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Argentinean Propolis
Antifungal Activity
Dermatophytes
Candida
Lignans
Hplc–Esi-Ms/Ms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29712
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytesAguero, María BelenSvetaz, Laura AndreaBaroni, María VerónicaLima, Beatriz VivianaLuna, Lorena CelinaZacchino, Susana Alicia StellaSaavedra, PedroWunderlin, Daniel AlbertoFeresin, Gabriela EglyTapia, Aníbal AlejandroArgentinean PropolisAntifungal ActivityDermatophytesCandidaLignansHplc–Esi-Ms/Mshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Propolis is widely used in the folk medicine of San Juan province (Argentina) to treat several diseases, including cold, cough, muscle aches and superficial mycoses. We report the in vitro antifungal activity of urban propolis, evaluated with CLSI protocols in addition to the evaluation of their chemical profile by HPLC-ESI-MS/MS techniques. The dermatophytes Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum were the most susceptible species and guided the fractionation of urban propolis, which was performed with Sephadex LH-20 leading to eight fractions (I–VIII). These fractions showed high antifungal activities against dermatophytes (MICs = 16.0–62.5 μg/mL) and yeasts (MICs = 31.2–125 μg/mL) being III, V and VI the most active ones (MIC100 = 16–31.2 μg/mL). They also, showed fungicidal capacity (a condition highly appreciated in antifungal drugs to avoid recurrence) with MFC values between 31.2 and 62.5 μg/mL. From the most active fractions, two lignans: 3′-methyl-nordihydroguaiaretic acid (MNDGA) (1), and nordihydroguaiaretic acid (NDGA) (2), in addition to three flavonoids: chrysin (3), pinocembrin (4) and galangin (5), were isolated and quantified by HPLC–PDA-MS/MS as the main antifungal compounds. Lignans 1 and 2 showed strong activities against T. mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 31.2 and 62.5 μg/mL), and 1 showed strong activity against Candida albicans, Candida tropicalis and Cryptococcus neoformans (MICs between 31.2 and 62.5 μg/mL). Regarding flavonoids, all yeasts were sensitive to 5 (MIC = 31.2 μg/mL), whereas the dermatophytes T. mentagrophytes and T. rubrum and all yeasts were moderately inhibited by 4 (MIC = 31.2–250 μg/mL). Finally, chrysin (3) showed low activity against yeasts and dermatophytes (MIC = 250 μg/mL). These results support that Argentinean urban propolis, which are frequently used by beekeepers for the preparation of syrups, tinctures and creams, are valuable natural product for the improving of human health, particularly fungal infections. It is also worthy to take into account that its chemical composition contains mainly two antifungal lignans, associated with the medicinal Larrea genusFil: Aguero, María Belen. Universidad Nacional de San Juan; ArgentinaFil: Svetaz, Laura Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Baroni, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Lima, Beatriz Viviana. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luna, Lorena Celina. Universidad Nacional de San Juan; ArgentinaFil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Saavedra, Pedro. APICENTRO; ArgentinaFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tapia, Aníbal Alejandro. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29712Aguero, María Belen; Svetaz, Laura Andrea; Baroni, María Verónica; Lima, Beatriz Viviana; Luna, Lorena Celina; et al.; Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes; Elsevier Science; Industrial Crops and Products; 57; 3-2014; 166-1730926-6690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0926669014001435info:eu-repo/semantics/altIdentifier/doi/10.1016/j.indcrop.2014.03.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:34Zoai:ri.conicet.gov.ar:11336/29712instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:34.401CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
title Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
spellingShingle Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
Aguero, María Belen
Argentinean Propolis
Antifungal Activity
Dermatophytes
Candida
Lignans
Hplc–Esi-Ms/Ms
title_short Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
title_full Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
title_fullStr Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
title_full_unstemmed Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
title_sort Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes
dc.creator.none.fl_str_mv Aguero, María Belen
Svetaz, Laura Andrea
Baroni, María Verónica
Lima, Beatriz Viviana
Luna, Lorena Celina
Zacchino, Susana Alicia Stella
Saavedra, Pedro
Wunderlin, Daniel Alberto
Feresin, Gabriela Egly
Tapia, Aníbal Alejandro
author Aguero, María Belen
author_facet Aguero, María Belen
Svetaz, Laura Andrea
Baroni, María Verónica
Lima, Beatriz Viviana
Luna, Lorena Celina
Zacchino, Susana Alicia Stella
Saavedra, Pedro
Wunderlin, Daniel Alberto
Feresin, Gabriela Egly
Tapia, Aníbal Alejandro
author_role author
author2 Svetaz, Laura Andrea
Baroni, María Verónica
Lima, Beatriz Viviana
Luna, Lorena Celina
Zacchino, Susana Alicia Stella
Saavedra, Pedro
Wunderlin, Daniel Alberto
Feresin, Gabriela Egly
Tapia, Aníbal Alejandro
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Argentinean Propolis
Antifungal Activity
Dermatophytes
Candida
Lignans
Hplc–Esi-Ms/Ms
topic Argentinean Propolis
Antifungal Activity
Dermatophytes
Candida
Lignans
Hplc–Esi-Ms/Ms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Propolis is widely used in the folk medicine of San Juan province (Argentina) to treat several diseases, including cold, cough, muscle aches and superficial mycoses. We report the in vitro antifungal activity of urban propolis, evaluated with CLSI protocols in addition to the evaluation of their chemical profile by HPLC-ESI-MS/MS techniques. The dermatophytes Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum were the most susceptible species and guided the fractionation of urban propolis, which was performed with Sephadex LH-20 leading to eight fractions (I–VIII). These fractions showed high antifungal activities against dermatophytes (MICs = 16.0–62.5 μg/mL) and yeasts (MICs = 31.2–125 μg/mL) being III, V and VI the most active ones (MIC100 = 16–31.2 μg/mL). They also, showed fungicidal capacity (a condition highly appreciated in antifungal drugs to avoid recurrence) with MFC values between 31.2 and 62.5 μg/mL. From the most active fractions, two lignans: 3′-methyl-nordihydroguaiaretic acid (MNDGA) (1), and nordihydroguaiaretic acid (NDGA) (2), in addition to three flavonoids: chrysin (3), pinocembrin (4) and galangin (5), were isolated and quantified by HPLC–PDA-MS/MS as the main antifungal compounds. Lignans 1 and 2 showed strong activities against T. mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 31.2 and 62.5 μg/mL), and 1 showed strong activity against Candida albicans, Candida tropicalis and Cryptococcus neoformans (MICs between 31.2 and 62.5 μg/mL). Regarding flavonoids, all yeasts were sensitive to 5 (MIC = 31.2 μg/mL), whereas the dermatophytes T. mentagrophytes and T. rubrum and all yeasts were moderately inhibited by 4 (MIC = 31.2–250 μg/mL). Finally, chrysin (3) showed low activity against yeasts and dermatophytes (MIC = 250 μg/mL). These results support that Argentinean urban propolis, which are frequently used by beekeepers for the preparation of syrups, tinctures and creams, are valuable natural product for the improving of human health, particularly fungal infections. It is also worthy to take into account that its chemical composition contains mainly two antifungal lignans, associated with the medicinal Larrea genus
Fil: Aguero, María Belen. Universidad Nacional de San Juan; Argentina
Fil: Svetaz, Laura Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Baroni, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luna, Lorena Celina. Universidad Nacional de San Juan; Argentina
Fil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saavedra, Pedro. APICENTRO; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tapia, Aníbal Alejandro. Universidad Nacional de San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Propolis is widely used in the folk medicine of San Juan province (Argentina) to treat several diseases, including cold, cough, muscle aches and superficial mycoses. We report the in vitro antifungal activity of urban propolis, evaluated with CLSI protocols in addition to the evaluation of their chemical profile by HPLC-ESI-MS/MS techniques. The dermatophytes Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum were the most susceptible species and guided the fractionation of urban propolis, which was performed with Sephadex LH-20 leading to eight fractions (I–VIII). These fractions showed high antifungal activities against dermatophytes (MICs = 16.0–62.5 μg/mL) and yeasts (MICs = 31.2–125 μg/mL) being III, V and VI the most active ones (MIC100 = 16–31.2 μg/mL). They also, showed fungicidal capacity (a condition highly appreciated in antifungal drugs to avoid recurrence) with MFC values between 31.2 and 62.5 μg/mL. From the most active fractions, two lignans: 3′-methyl-nordihydroguaiaretic acid (MNDGA) (1), and nordihydroguaiaretic acid (NDGA) (2), in addition to three flavonoids: chrysin (3), pinocembrin (4) and galangin (5), were isolated and quantified by HPLC–PDA-MS/MS as the main antifungal compounds. Lignans 1 and 2 showed strong activities against T. mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 31.2 and 62.5 μg/mL), and 1 showed strong activity against Candida albicans, Candida tropicalis and Cryptococcus neoformans (MICs between 31.2 and 62.5 μg/mL). Regarding flavonoids, all yeasts were sensitive to 5 (MIC = 31.2 μg/mL), whereas the dermatophytes T. mentagrophytes and T. rubrum and all yeasts were moderately inhibited by 4 (MIC = 31.2–250 μg/mL). Finally, chrysin (3) showed low activity against yeasts and dermatophytes (MIC = 250 μg/mL). These results support that Argentinean urban propolis, which are frequently used by beekeepers for the preparation of syrups, tinctures and creams, are valuable natural product for the improving of human health, particularly fungal infections. It is also worthy to take into account that its chemical composition contains mainly two antifungal lignans, associated with the medicinal Larrea genus
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29712
Aguero, María Belen; Svetaz, Laura Andrea; Baroni, María Verónica; Lima, Beatriz Viviana; Luna, Lorena Celina; et al.; Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes; Elsevier Science; Industrial Crops and Products; 57; 3-2014; 166-173
0926-6690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29712
identifier_str_mv Aguero, María Belen; Svetaz, Laura Andrea; Baroni, María Verónica; Lima, Beatriz Viviana; Luna, Lorena Celina; et al.; Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes; Elsevier Science; Industrial Crops and Products; 57; 3-2014; 166-173
0926-6690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0926669014001435
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.indcrop.2014.03.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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