GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection

Autores
Ruiz, Vanesa; Giordano, Mirta Nilda; Rivero, Cintia Wanda; Minassian, Maria Laura; Cuestas, María Luján; Trinks, Julieta; Mathet, Veronica Lidia; Oubiña, Jose Raul
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genomic heterogeneity and quasispecies composition of GB virus C (GBV-C) within plasma and lymphocyte subsets in a naturally infected blood donor were investigated. For this purpose, fragments from the 5′ untranslated region (5′ UTR) and the E2 gene recovered from plasma, B and T lymphocytes, were cloned and sequenced. A total of 63 clones was analysed: 95.2% of them (n=60) - obtained from plasma and cells - were assigned to genotype 2b, while only three derived from plasma corresponded to genotyope 3. The G215A transition within this region was present in 90.9% of the clones from B lymphocytes, but absent in the remaining cell compartments (P<0.01). Apparently, most of the circulating GBV-C quasispecies in this blood donor were related to the viral population infecting CD8+ T cells, and B cells to a lesser extent. This is the first report showing the quasispecies nature of GBV-C in lymphocyte subsets within peripheral blood mononuclear cells. © 2010 SGM.
Fil: Ruiz, Vanesa. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giordano, Mirta Nilda. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivero, Cintia Wanda. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Minassian, Maria Laura. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trinks, Julieta. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Gb Virus C
Quasispecies
Plasma
Lymphocyte
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62369
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62369
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infectionRuiz, VanesaGiordano, Mirta NildaRivero, Cintia WandaMinassian, Maria LauraCuestas, María LujánTrinks, JulietaMathet, Veronica LidiaOubiña, Jose RaulGb Virus CQuasispeciesPlasmaLymphocytehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Genomic heterogeneity and quasispecies composition of GB virus C (GBV-C) within plasma and lymphocyte subsets in a naturally infected blood donor were investigated. For this purpose, fragments from the 5′ untranslated region (5′ UTR) and the E2 gene recovered from plasma, B and T lymphocytes, were cloned and sequenced. A total of 63 clones was analysed: 95.2% of them (n=60) - obtained from plasma and cells - were assigned to genotype 2b, while only three derived from plasma corresponded to genotyope 3. The G215A transition within this region was present in 90.9% of the clones from B lymphocytes, but absent in the remaining cell compartments (P<0.01). Apparently, most of the circulating GBV-C quasispecies in this blood donor were related to the viral population infecting CD8+ T cells, and B cells to a lesser extent. This is the first report showing the quasispecies nature of GBV-C in lymphocyte subsets within peripheral blood mononuclear cells. © 2010 SGM.Fil: Ruiz, Vanesa. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giordano, Mirta Nilda. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivero, Cintia Wanda. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Minassian, Maria Laura. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trinks, Julieta. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSociety for General Microbiology2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62369Ruiz, Vanesa; Giordano, Mirta Nilda; Rivero, Cintia Wanda; Minassian, Maria Laura; Cuestas, María Luján; et al.; GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection; Society for General Microbiology; Journal of General Virology; 91; 7; 7-2010; 1687-16920022-1317CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.019877-0info:eu-repo/semantics/altIdentifier/url/http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.019877-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:12:47Zoai:ri.conicet.gov.ar:11336/62369instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:12:47.816CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
title GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
spellingShingle GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
Ruiz, Vanesa
Gb Virus C
Quasispecies
Plasma
Lymphocyte
title_short GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
title_full GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
title_fullStr GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
title_full_unstemmed GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
title_sort GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection
dc.creator.none.fl_str_mv Ruiz, Vanesa
Giordano, Mirta Nilda
Rivero, Cintia Wanda
Minassian, Maria Laura
Cuestas, María Luján
Trinks, Julieta
Mathet, Veronica Lidia
Oubiña, Jose Raul
author Ruiz, Vanesa
author_facet Ruiz, Vanesa
Giordano, Mirta Nilda
Rivero, Cintia Wanda
Minassian, Maria Laura
Cuestas, María Luján
Trinks, Julieta
Mathet, Veronica Lidia
Oubiña, Jose Raul
author_role author
author2 Giordano, Mirta Nilda
Rivero, Cintia Wanda
Minassian, Maria Laura
Cuestas, María Luján
Trinks, Julieta
Mathet, Veronica Lidia
Oubiña, Jose Raul
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gb Virus C
Quasispecies
Plasma
Lymphocyte
topic Gb Virus C
Quasispecies
Plasma
Lymphocyte
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Genomic heterogeneity and quasispecies composition of GB virus C (GBV-C) within plasma and lymphocyte subsets in a naturally infected blood donor were investigated. For this purpose, fragments from the 5′ untranslated region (5′ UTR) and the E2 gene recovered from plasma, B and T lymphocytes, were cloned and sequenced. A total of 63 clones was analysed: 95.2% of them (n=60) - obtained from plasma and cells - were assigned to genotype 2b, while only three derived from plasma corresponded to genotyope 3. The G215A transition within this region was present in 90.9% of the clones from B lymphocytes, but absent in the remaining cell compartments (P<0.01). Apparently, most of the circulating GBV-C quasispecies in this blood donor were related to the viral population infecting CD8+ T cells, and B cells to a lesser extent. This is the first report showing the quasispecies nature of GBV-C in lymphocyte subsets within peripheral blood mononuclear cells. © 2010 SGM.
Fil: Ruiz, Vanesa. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giordano, Mirta Nilda. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivero, Cintia Wanda. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Minassian, Maria Laura. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trinks, Julieta. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Genomic heterogeneity and quasispecies composition of GB virus C (GBV-C) within plasma and lymphocyte subsets in a naturally infected blood donor were investigated. For this purpose, fragments from the 5′ untranslated region (5′ UTR) and the E2 gene recovered from plasma, B and T lymphocytes, were cloned and sequenced. A total of 63 clones was analysed: 95.2% of them (n=60) - obtained from plasma and cells - were assigned to genotype 2b, while only three derived from plasma corresponded to genotyope 3. The G215A transition within this region was present in 90.9% of the clones from B lymphocytes, but absent in the remaining cell compartments (P<0.01). Apparently, most of the circulating GBV-C quasispecies in this blood donor were related to the viral population infecting CD8+ T cells, and B cells to a lesser extent. This is the first report showing the quasispecies nature of GBV-C in lymphocyte subsets within peripheral blood mononuclear cells. © 2010 SGM.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62369
Ruiz, Vanesa; Giordano, Mirta Nilda; Rivero, Cintia Wanda; Minassian, Maria Laura; Cuestas, María Luján; et al.; GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection; Society for General Microbiology; Journal of General Virology; 91; 7; 7-2010; 1687-1692
0022-1317
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62369
identifier_str_mv Ruiz, Vanesa; Giordano, Mirta Nilda; Rivero, Cintia Wanda; Minassian, Maria Laura; Cuestas, María Luján; et al.; GB virus C quasispecies detected in plasma and lymphocyte subsets in a natural human infection; Society for General Microbiology; Journal of General Virology; 91; 7; 7-2010; 1687-1692
0022-1317
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.019877-0
info:eu-repo/semantics/altIdentifier/url/http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.019877-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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