Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin

Autores
Castillo, Graciela; Chanampa, Yolanda; Orce, Gabriel Guillermo F.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The amphibian skin, widely used for studying the transepithelial passage of electrolytes, exhibits anion pathways relatively specific for Cl-. We studied the effect of HgCl2, 1.0×10-4 M on its electrical parameters and unidirectional anion fluxes. In the presence of Cl-, the transepithelial conductance (G) of the isolated skin of the Bufo arenarum toad increased considerably following exposure to HgCl 2, whereas short-circuit current (SCC)-reflecting transepithelial Na+ transport-underwent only slight stimulation. Following the blockade of Na+ intake by amiloride, 1.0×10-4 M, the removal of Cl- from the solution bathing the epidermal border of the skin brought about a decrease in G, and gave rise to a gradient-induced SCC (SCCg) consistent with transepithelial passage of Cl- along its gradient. Addition of mercaptoethanol, 5.0×10-3 M to the bath containing Hg2+ fully reversed these effects. The increase in G was accompanied by an increase in the unidirectional (epidermal to dermal) fluxes of 36Cl- and 131I-, and a decrease in the passage of 99mTcO4-. These results show the effects of HgCl2 to be similar to those of theophylline, although exhibiting a different selectivity. Our data suggest that anion passage following exposure to HgCl2 is, like that stimulated by theophylline, predominantly if not exclusively transcellular, and does not involve a significant opening of the tight junctions.
Fil: Castillo, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Chanampa, Yolanda. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Orce, Gabriel Guillermo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
131I -
36CL-
99MTCO4-
BUFO ARENARUM
EPITHELIAL ANION PERMEABILITY
SHORT-CIRCUIT CURRENT
TOAD SKIN
TRANSEPITHELIAL CONDUCTANCE
UNIDIRECTIONAL FLUXES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95101
Acceder
id CONICETDig_daf1b9d98ba746afa77a12e74da03cdd
oai_identifier_str oai:ri.conicet.gov.ar:11336/95101
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skinCastillo, GracielaChanampa, YolandaOrce, Gabriel Guillermo F.131I -36CL-99MTCO4-BUFO ARENARUMEPITHELIAL ANION PERMEABILITYSHORT-CIRCUIT CURRENTTOAD SKINTRANSEPITHELIAL CONDUCTANCEUNIDIRECTIONAL FLUXEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The amphibian skin, widely used for studying the transepithelial passage of electrolytes, exhibits anion pathways relatively specific for Cl-. We studied the effect of HgCl2, 1.0×10-4 M on its electrical parameters and unidirectional anion fluxes. In the presence of Cl-, the transepithelial conductance (G) of the isolated skin of the Bufo arenarum toad increased considerably following exposure to HgCl 2, whereas short-circuit current (SCC)-reflecting transepithelial Na+ transport-underwent only slight stimulation. Following the blockade of Na+ intake by amiloride, 1.0×10-4 M, the removal of Cl- from the solution bathing the epidermal border of the skin brought about a decrease in G, and gave rise to a gradient-induced SCC (SCCg) consistent with transepithelial passage of Cl- along its gradient. Addition of mercaptoethanol, 5.0×10-3 M to the bath containing Hg2+ fully reversed these effects. The increase in G was accompanied by an increase in the unidirectional (epidermal to dermal) fluxes of 36Cl- and 131I-, and a decrease in the passage of 99mTcO4-. These results show the effects of HgCl2 to be similar to those of theophylline, although exhibiting a different selectivity. Our data suggest that anion passage following exposure to HgCl2 is, like that stimulated by theophylline, predominantly if not exclusively transcellular, and does not involve a significant opening of the tight junctions.Fil: Castillo, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Chanampa, Yolanda. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Orce, Gabriel Guillermo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier Science Inc2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/95101Castillo, Graciela; Chanampa, Yolanda; Orce, Gabriel Guillermo F.; Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 140; 1; 12-2005; 21-270742-84131532-0456CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cca.2004.12.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045604002509info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:07Zoai:ri.conicet.gov.ar:11336/95101instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:07.436CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
title Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
spellingShingle Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
Castillo, Graciela
131I -
36CL-
99MTCO4-
BUFO ARENARUM
EPITHELIAL ANION PERMEABILITY
SHORT-CIRCUIT CURRENT
TOAD SKIN
TRANSEPITHELIAL CONDUCTANCE
UNIDIRECTIONAL FLUXES
title_short Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
title_full Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
title_fullStr Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
title_full_unstemmed Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
title_sort Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin
dc.creator.none.fl_str_mv Castillo, Graciela
Chanampa, Yolanda
Orce, Gabriel Guillermo F.
author Castillo, Graciela
author_facet Castillo, Graciela
Chanampa, Yolanda
Orce, Gabriel Guillermo F.
author_role author
author2 Chanampa, Yolanda
Orce, Gabriel Guillermo F.
author2_role author
author
dc.subject.none.fl_str_mv 131I -
36CL-
99MTCO4-
BUFO ARENARUM
EPITHELIAL ANION PERMEABILITY
SHORT-CIRCUIT CURRENT
TOAD SKIN
TRANSEPITHELIAL CONDUCTANCE
UNIDIRECTIONAL FLUXES
topic 131I -
36CL-
99MTCO4-
BUFO ARENARUM
EPITHELIAL ANION PERMEABILITY
SHORT-CIRCUIT CURRENT
TOAD SKIN
TRANSEPITHELIAL CONDUCTANCE
UNIDIRECTIONAL FLUXES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The amphibian skin, widely used for studying the transepithelial passage of electrolytes, exhibits anion pathways relatively specific for Cl-. We studied the effect of HgCl2, 1.0×10-4 M on its electrical parameters and unidirectional anion fluxes. In the presence of Cl-, the transepithelial conductance (G) of the isolated skin of the Bufo arenarum toad increased considerably following exposure to HgCl 2, whereas short-circuit current (SCC)-reflecting transepithelial Na+ transport-underwent only slight stimulation. Following the blockade of Na+ intake by amiloride, 1.0×10-4 M, the removal of Cl- from the solution bathing the epidermal border of the skin brought about a decrease in G, and gave rise to a gradient-induced SCC (SCCg) consistent with transepithelial passage of Cl- along its gradient. Addition of mercaptoethanol, 5.0×10-3 M to the bath containing Hg2+ fully reversed these effects. The increase in G was accompanied by an increase in the unidirectional (epidermal to dermal) fluxes of 36Cl- and 131I-, and a decrease in the passage of 99mTcO4-. These results show the effects of HgCl2 to be similar to those of theophylline, although exhibiting a different selectivity. Our data suggest that anion passage following exposure to HgCl2 is, like that stimulated by theophylline, predominantly if not exclusively transcellular, and does not involve a significant opening of the tight junctions.
Fil: Castillo, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Chanampa, Yolanda. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Orce, Gabriel Guillermo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description The amphibian skin, widely used for studying the transepithelial passage of electrolytes, exhibits anion pathways relatively specific for Cl-. We studied the effect of HgCl2, 1.0×10-4 M on its electrical parameters and unidirectional anion fluxes. In the presence of Cl-, the transepithelial conductance (G) of the isolated skin of the Bufo arenarum toad increased considerably following exposure to HgCl 2, whereas short-circuit current (SCC)-reflecting transepithelial Na+ transport-underwent only slight stimulation. Following the blockade of Na+ intake by amiloride, 1.0×10-4 M, the removal of Cl- from the solution bathing the epidermal border of the skin brought about a decrease in G, and gave rise to a gradient-induced SCC (SCCg) consistent with transepithelial passage of Cl- along its gradient. Addition of mercaptoethanol, 5.0×10-3 M to the bath containing Hg2+ fully reversed these effects. The increase in G was accompanied by an increase in the unidirectional (epidermal to dermal) fluxes of 36Cl- and 131I-, and a decrease in the passage of 99mTcO4-. These results show the effects of HgCl2 to be similar to those of theophylline, although exhibiting a different selectivity. Our data suggest that anion passage following exposure to HgCl2 is, like that stimulated by theophylline, predominantly if not exclusively transcellular, and does not involve a significant opening of the tight junctions.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95101
Castillo, Graciela; Chanampa, Yolanda; Orce, Gabriel Guillermo F.; Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 140; 1; 12-2005; 21-27
0742-8413
1532-0456
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95101
identifier_str_mv Castillo, Graciela; Chanampa, Yolanda; Orce, Gabriel Guillermo F.; Effect of mercuric chloride on electrical parameters and anion fluxes in the toad skin; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 140; 1; 12-2005; 21-27
0742-8413
1532-0456
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cca.2004.12.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045604002509
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782135204052992
score 12.982451

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