Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats

Autores
Delfini, Claudio Daniel; Villegas, Liliana Beatriz; Martinez, Maria Alejandra; Baigori, Mario Domingo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A reduction in the use of agrochemicals requires the development of either alternatives or complementary control methods in order to limit their dangerousness. An alternative is the biological control of fungi by bacteria. The fungal cell wall is a unique structure of the fungi, composed of glucan, chitin, and glycoproteins. Therefore, bacteria producing mycolytic enzymes, like chitinases, are of great interest to degrade fungal cell-wall components. The objectives of this work were to isolate chitinolytic bacteria from the guano of insectivorous bats (Tadarida brasiliensis) and to verify the presence of antifungal activities against phytopathogenic fungi. From the guano samples, 28 bacterial isolates were obtained, 70% of which presented chitinolytic activity. Four isolates were selected since they showed the highest values of chitinase activity, and they were characterized as belonging to Bacillus genus, by analyzing the 16S ribosomal RNA gene sequence. Cell-free supernatants of bacterial cultures were used in inhibition tests on 16 fungi: Alternaria and Colletotrichum acutatum were the most affected. Chitinase and antifungal activities were observed in the cell-free supernatant regardless of the culture medium used. Both activities were stable to heat and proteinase K treatments. Finally, when the culture medium was supplemented with 1 ml of cell-free supernatants (0.33%) and incubated for 120 h, the inhibition of hyphae formation and germination spores of reporter fungus were observed under light microscopy. These results suggest the feasibility of using cell-free supernatants as eco-friendly fungicides. The use of them may contribute to reducing the dose of toxic chemicals.
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
Materia
Chitinase
Antifungal Activity
Bacteria
Insectivorous Bats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141438
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous BatsDelfini, Claudio DanielVillegas, Liliana BeatrizMartinez, Maria AlejandraBaigori, Mario DomingoChitinaseAntifungal ActivityBacteriaInsectivorous Batshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2A reduction in the use of agrochemicals requires the development of either alternatives or complementary control methods in order to limit their dangerousness. An alternative is the biological control of fungi by bacteria. The fungal cell wall is a unique structure of the fungi, composed of glucan, chitin, and glycoproteins. Therefore, bacteria producing mycolytic enzymes, like chitinases, are of great interest to degrade fungal cell-wall components. The objectives of this work were to isolate chitinolytic bacteria from the guano of insectivorous bats (Tadarida brasiliensis) and to verify the presence of antifungal activities against phytopathogenic fungi. From the guano samples, 28 bacterial isolates were obtained, 70% of which presented chitinolytic activity. Four isolates were selected since they showed the highest values of chitinase activity, and they were characterized as belonging to Bacillus genus, by analyzing the 16S ribosomal RNA gene sequence. Cell-free supernatants of bacterial cultures were used in inhibition tests on 16 fungi: Alternaria and Colletotrichum acutatum were the most affected. Chitinase and antifungal activities were observed in the cell-free supernatant regardless of the culture medium used. Both activities were stable to heat and proteinase K treatments. Finally, when the culture medium was supplemented with 1 ml of cell-free supernatants (0.33%) and incubated for 120 h, the inhibition of hyphae formation and germination spores of reporter fungus were observed under light microscopy. These results suggest the feasibility of using cell-free supernatants as eco-friendly fungicides. The use of them may contribute to reducing the dose of toxic chemicals.Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; ArgentinaSpringer2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141438Delfini, Claudio Daniel; Villegas, Liliana Beatriz; Martinez, Maria Alejandra; Baigori, Mario Domingo; Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats; Springer; Current Microbiology; 78; 7; 7-2021; 2787-27980343-8651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-021-02555-0info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00284-021-02555-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:14Zoai:ri.conicet.gov.ar:11336/141438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:14.866CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
title Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
spellingShingle Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
Delfini, Claudio Daniel
Chitinase
Antifungal Activity
Bacteria
Insectivorous Bats
title_short Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
title_full Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
title_fullStr Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
title_full_unstemmed Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
title_sort Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
dc.creator.none.fl_str_mv Delfini, Claudio Daniel
Villegas, Liliana Beatriz
Martinez, Maria Alejandra
Baigori, Mario Domingo
author Delfini, Claudio Daniel
author_facet Delfini, Claudio Daniel
Villegas, Liliana Beatriz
Martinez, Maria Alejandra
Baigori, Mario Domingo
author_role author
author2 Villegas, Liliana Beatriz
Martinez, Maria Alejandra
Baigori, Mario Domingo
author2_role author
author
author
dc.subject.none.fl_str_mv Chitinase
Antifungal Activity
Bacteria
Insectivorous Bats
topic Chitinase
Antifungal Activity
Bacteria
Insectivorous Bats
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A reduction in the use of agrochemicals requires the development of either alternatives or complementary control methods in order to limit their dangerousness. An alternative is the biological control of fungi by bacteria. The fungal cell wall is a unique structure of the fungi, composed of glucan, chitin, and glycoproteins. Therefore, bacteria producing mycolytic enzymes, like chitinases, are of great interest to degrade fungal cell-wall components. The objectives of this work were to isolate chitinolytic bacteria from the guano of insectivorous bats (Tadarida brasiliensis) and to verify the presence of antifungal activities against phytopathogenic fungi. From the guano samples, 28 bacterial isolates were obtained, 70% of which presented chitinolytic activity. Four isolates were selected since they showed the highest values of chitinase activity, and they were characterized as belonging to Bacillus genus, by analyzing the 16S ribosomal RNA gene sequence. Cell-free supernatants of bacterial cultures were used in inhibition tests on 16 fungi: Alternaria and Colletotrichum acutatum were the most affected. Chitinase and antifungal activities were observed in the cell-free supernatant regardless of the culture medium used. Both activities were stable to heat and proteinase K treatments. Finally, when the culture medium was supplemented with 1 ml of cell-free supernatants (0.33%) and incubated for 120 h, the inhibition of hyphae formation and germination spores of reporter fungus were observed under light microscopy. These results suggest the feasibility of using cell-free supernatants as eco-friendly fungicides. The use of them may contribute to reducing the dose of toxic chemicals.
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
description A reduction in the use of agrochemicals requires the development of either alternatives or complementary control methods in order to limit their dangerousness. An alternative is the biological control of fungi by bacteria. The fungal cell wall is a unique structure of the fungi, composed of glucan, chitin, and glycoproteins. Therefore, bacteria producing mycolytic enzymes, like chitinases, are of great interest to degrade fungal cell-wall components. The objectives of this work were to isolate chitinolytic bacteria from the guano of insectivorous bats (Tadarida brasiliensis) and to verify the presence of antifungal activities against phytopathogenic fungi. From the guano samples, 28 bacterial isolates were obtained, 70% of which presented chitinolytic activity. Four isolates were selected since they showed the highest values of chitinase activity, and they were characterized as belonging to Bacillus genus, by analyzing the 16S ribosomal RNA gene sequence. Cell-free supernatants of bacterial cultures were used in inhibition tests on 16 fungi: Alternaria and Colletotrichum acutatum were the most affected. Chitinase and antifungal activities were observed in the cell-free supernatant regardless of the culture medium used. Both activities were stable to heat and proteinase K treatments. Finally, when the culture medium was supplemented with 1 ml of cell-free supernatants (0.33%) and incubated for 120 h, the inhibition of hyphae formation and germination spores of reporter fungus were observed under light microscopy. These results suggest the feasibility of using cell-free supernatants as eco-friendly fungicides. The use of them may contribute to reducing the dose of toxic chemicals.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141438
Delfini, Claudio Daniel; Villegas, Liliana Beatriz; Martinez, Maria Alejandra; Baigori, Mario Domingo; Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats; Springer; Current Microbiology; 78; 7; 7-2021; 2787-2798
0343-8651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141438
identifier_str_mv Delfini, Claudio Daniel; Villegas, Liliana Beatriz; Martinez, Maria Alejandra; Baigori, Mario Domingo; Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats; Springer; Current Microbiology; 78; 7; 7-2021; 2787-2798
0343-8651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-021-02555-0
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00284-021-02555-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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