Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest

Autores
Bun Wong, Thomas Chi; Rebbert, Martha; Wang, Chengdong; Chen, Xiongfong; Heffer, Alison; Zarelli, Valeria Eugenia Paola; Dawid, Igor B.; Zhao, Hui
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neural crest (NC) development is controlled precisely by a regulatory network with multiple signaling pathways and the involvement of many genes. The integration and coordination of these factors are still incompletely understood. Overexpression of Wnt3a and the BMP antagonist Chordin in animal cap cells from Xenopus blastulae induces a large number of NC specific genes. We previously suggested that Potassium Channel Tetramerization Domain containing 15 (Kctd15) regulates NC formation by affecting Wnt signaling and the activity of transcription factor AP-2. In order to advance understanding of the function of Kctd15 during NC development, we performed DNA microarray assays in explants injected with Wnt3a and Chordin, and identified genes that are affected by Kctd15 overexpression. Among the many genes identified, we chose Duf domain containing protein 1 (ddcp1), Platelet-Derived Growth Factor Receptor a (pdgfra), Complement factor properdin (cfp), Zinc Finger SWIM-Type Containing 5 (zswim5), and complement component 3 (C3) to examine their expression by whole mount in situ hybridization. Our work points to a possible role for Kctd15 in the regulation of NC formation and other steps in embryonic development.
Fil: Bun Wong, Thomas Chi. The Chinese University of Hong Kong; China
Fil: Rebbert, Martha. National Institutes of Health; Estados Unidos
Fil: Wang, Chengdong. The Chinese University of Hong Kong; China
Fil: Chen, Xiongfong. National Institutes of Health; Estados Unidos
Fil: Heffer, Alison. National Institutes of Health; Estados Unidos
Fil: Zarelli, Valeria Eugenia Paola. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Dawid, Igor B.. National Institutes of Health; Estados Unidos
Fil: Zhao, Hui. The Chinese University of Hong Kong; China. Chinese Academy of Sciences; República de China
Materia
DNA MICROARRAY
GENE REGULATION
KCTD15
NEURAL CREST
TRANSCRIPTION FACTOR AP-2
XENOPUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48923
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48923
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crestBun Wong, Thomas ChiRebbert, MarthaWang, ChengdongChen, XiongfongHeffer, AlisonZarelli, Valeria Eugenia PaolaDawid, Igor B.Zhao, HuiDNA MICROARRAYGENE REGULATIONKCTD15NEURAL CRESTTRANSCRIPTION FACTOR AP-2XENOPUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neural crest (NC) development is controlled precisely by a regulatory network with multiple signaling pathways and the involvement of many genes. The integration and coordination of these factors are still incompletely understood. Overexpression of Wnt3a and the BMP antagonist Chordin in animal cap cells from Xenopus blastulae induces a large number of NC specific genes. We previously suggested that Potassium Channel Tetramerization Domain containing 15 (Kctd15) regulates NC formation by affecting Wnt signaling and the activity of transcription factor AP-2. In order to advance understanding of the function of Kctd15 during NC development, we performed DNA microarray assays in explants injected with Wnt3a and Chordin, and identified genes that are affected by Kctd15 overexpression. Among the many genes identified, we chose Duf domain containing protein 1 (ddcp1), Platelet-Derived Growth Factor Receptor a (pdgfra), Complement factor properdin (cfp), Zinc Finger SWIM-Type Containing 5 (zswim5), and complement component 3 (C3) to examine their expression by whole mount in situ hybridization. Our work points to a possible role for Kctd15 in the regulation of NC formation and other steps in embryonic development.Fil: Bun Wong, Thomas Chi. The Chinese University of Hong Kong; ChinaFil: Rebbert, Martha. National Institutes of Health; Estados UnidosFil: Wang, Chengdong. The Chinese University of Hong Kong; ChinaFil: Chen, Xiongfong. National Institutes of Health; Estados UnidosFil: Heffer, Alison. National Institutes of Health; Estados UnidosFil: Zarelli, Valeria Eugenia Paola. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Dawid, Igor B.. National Institutes of Health; Estados UnidosFil: Zhao, Hui. The Chinese University of Hong Kong; China. Chinese Academy of Sciences; República de ChinaU B C Press2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48923Bun Wong, Thomas Chi; Rebbert, Martha; Wang, Chengdong; Chen, Xiongfong; Heffer, Alison; et al.; Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest; U B C Press; International Journal Of Developmental Biology; 60; 4-6; 4-2016; 159-1660214-6282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ijdb.ehu.es/web/paper.php?doi=160058idinfo:eu-repo/semantics/altIdentifier/doi/10.1387/ijdb.160058idinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:36:10Zoai:ri.conicet.gov.ar:11336/48923instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:36:11.04CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
title Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
spellingShingle Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
Bun Wong, Thomas Chi
DNA MICROARRAY
GENE REGULATION
KCTD15
NEURAL CREST
TRANSCRIPTION FACTOR AP-2
XENOPUS
title_short Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
title_full Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
title_fullStr Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
title_full_unstemmed Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
title_sort Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest
dc.creator.none.fl_str_mv Bun Wong, Thomas Chi
Rebbert, Martha
Wang, Chengdong
Chen, Xiongfong
Heffer, Alison
Zarelli, Valeria Eugenia Paola
Dawid, Igor B.
Zhao, Hui
author Bun Wong, Thomas Chi
author_facet Bun Wong, Thomas Chi
Rebbert, Martha
Wang, Chengdong
Chen, Xiongfong
Heffer, Alison
Zarelli, Valeria Eugenia Paola
Dawid, Igor B.
Zhao, Hui
author_role author
author2 Rebbert, Martha
Wang, Chengdong
Chen, Xiongfong
Heffer, Alison
Zarelli, Valeria Eugenia Paola
Dawid, Igor B.
Zhao, Hui
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DNA MICROARRAY
GENE REGULATION
KCTD15
NEURAL CREST
TRANSCRIPTION FACTOR AP-2
XENOPUS
topic DNA MICROARRAY
GENE REGULATION
KCTD15
NEURAL CREST
TRANSCRIPTION FACTOR AP-2
XENOPUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neural crest (NC) development is controlled precisely by a regulatory network with multiple signaling pathways and the involvement of many genes. The integration and coordination of these factors are still incompletely understood. Overexpression of Wnt3a and the BMP antagonist Chordin in animal cap cells from Xenopus blastulae induces a large number of NC specific genes. We previously suggested that Potassium Channel Tetramerization Domain containing 15 (Kctd15) regulates NC formation by affecting Wnt signaling and the activity of transcription factor AP-2. In order to advance understanding of the function of Kctd15 during NC development, we performed DNA microarray assays in explants injected with Wnt3a and Chordin, and identified genes that are affected by Kctd15 overexpression. Among the many genes identified, we chose Duf domain containing protein 1 (ddcp1), Platelet-Derived Growth Factor Receptor a (pdgfra), Complement factor properdin (cfp), Zinc Finger SWIM-Type Containing 5 (zswim5), and complement component 3 (C3) to examine their expression by whole mount in situ hybridization. Our work points to a possible role for Kctd15 in the regulation of NC formation and other steps in embryonic development.
Fil: Bun Wong, Thomas Chi. The Chinese University of Hong Kong; China
Fil: Rebbert, Martha. National Institutes of Health; Estados Unidos
Fil: Wang, Chengdong. The Chinese University of Hong Kong; China
Fil: Chen, Xiongfong. National Institutes of Health; Estados Unidos
Fil: Heffer, Alison. National Institutes of Health; Estados Unidos
Fil: Zarelli, Valeria Eugenia Paola. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Dawid, Igor B.. National Institutes of Health; Estados Unidos
Fil: Zhao, Hui. The Chinese University of Hong Kong; China. Chinese Academy of Sciences; República de China
description Neural crest (NC) development is controlled precisely by a regulatory network with multiple signaling pathways and the involvement of many genes. The integration and coordination of these factors are still incompletely understood. Overexpression of Wnt3a and the BMP antagonist Chordin in animal cap cells from Xenopus blastulae induces a large number of NC specific genes. We previously suggested that Potassium Channel Tetramerization Domain containing 15 (Kctd15) regulates NC formation by affecting Wnt signaling and the activity of transcription factor AP-2. In order to advance understanding of the function of Kctd15 during NC development, we performed DNA microarray assays in explants injected with Wnt3a and Chordin, and identified genes that are affected by Kctd15 overexpression. Among the many genes identified, we chose Duf domain containing protein 1 (ddcp1), Platelet-Derived Growth Factor Receptor a (pdgfra), Complement factor properdin (cfp), Zinc Finger SWIM-Type Containing 5 (zswim5), and complement component 3 (C3) to examine their expression by whole mount in situ hybridization. Our work points to a possible role for Kctd15 in the regulation of NC formation and other steps in embryonic development.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48923
Bun Wong, Thomas Chi; Rebbert, Martha; Wang, Chengdong; Chen, Xiongfong; Heffer, Alison; et al.; Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest; U B C Press; International Journal Of Developmental Biology; 60; 4-6; 4-2016; 159-166
0214-6282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48923
identifier_str_mv Bun Wong, Thomas Chi; Rebbert, Martha; Wang, Chengdong; Chen, Xiongfong; Heffer, Alison; et al.; Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest; U B C Press; International Journal Of Developmental Biology; 60; 4-6; 4-2016; 159-166
0214-6282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ijdb.ehu.es/web/paper.php?doi=160058id
info:eu-repo/semantics/altIdentifier/doi/10.1387/ijdb.160058id
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv U B C Press
publisher.none.fl_str_mv U B C Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.223955

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