Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes

Autores
Rodriguez, Ana María del Valle; Derita, Marcos Gabriel; Borkosky, Susana Alicia; Socolsky, Cecilia; Bardon, Alicia del Valle; Hernández, M.A.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The morphology and distribution of glandular trichomes in fronds at different developmental stages were investigated in Notholaena sulphurea, and the main components of the secreted material (farina) were identified. In addition antifungal and molluscicidal activities of farinose exudate were tested. Trichomes were examined using conventional light and scanning electron microscopy (SEM). The histochemical studies were conducted using the following tests on hand-sections: Toluidine Blue, Calcofluor White, Ruthenium Red, Phloroglucinol/HCl, NADI reagent, Sudan black B, Sudan IV, Nile Blue, Neutral Red, ferric chloride, Vainillin/H 2 SO 4 , Vainillin/HCl, alumimium chloride and Benedict reagent. Antifungal activity was evaluated by broth microdilution techniques and molluscicidal activity by Adenusi, 2008. One type of glandular trichomes was found in both the adaxial and the abaxial surfaces. Young fronds were densely covered with trichomes; however, the density decreased progressively with frond maturity. Secretions flowed by diffusion through cuticle micropores, and no rupturring mechanism to release secretions was observed. Histochemical reactions revealed that the main components of the materials secreted by trichomes include polysaccharides, lipids, terpenes, phenols and flavonoids. The farina showed antifungal and molluscicidal activity. Our results strongly support an ecological role of farina as herbivore deterrent and resistance to fungi. Since N. sulphurea is a xeric adapted fern, flavonoids present in farinous secretions may also function as stress protection against harsh environmental conditions.
Fil: Rodriguez, Ana María del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Derita, Marcos Gabriel. Universidad Nacional de Rosario; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Borkosky, Susana Alicia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Socolsky, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Hernández, M.A.. Fundación Miguel Lillo; Argentina
Materia
ANTIFUNGAL
FARINA
HISTOCHEMISTRY
MOLLUSCICIDAL
MORPHOLOGY
TRICHOMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91111
Acceder
id CONICETDig_daac3896e0cc515a7a42142d87545d36
oai_identifier_str oai:ri.conicet.gov.ar:11336/91111
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomesRodriguez, Ana María del ValleDerita, Marcos GabrielBorkosky, Susana AliciaSocolsky, CeciliaBardon, Alicia del ValleHernández, M.A.ANTIFUNGALFARINAHISTOCHEMISTRYMOLLUSCICIDALMORPHOLOGYTRICHOMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The morphology and distribution of glandular trichomes in fronds at different developmental stages were investigated in Notholaena sulphurea, and the main components of the secreted material (farina) were identified. In addition antifungal and molluscicidal activities of farinose exudate were tested. Trichomes were examined using conventional light and scanning electron microscopy (SEM). The histochemical studies were conducted using the following tests on hand-sections: Toluidine Blue, Calcofluor White, Ruthenium Red, Phloroglucinol/HCl, NADI reagent, Sudan black B, Sudan IV, Nile Blue, Neutral Red, ferric chloride, Vainillin/H 2 SO 4 , Vainillin/HCl, alumimium chloride and Benedict reagent. Antifungal activity was evaluated by broth microdilution techniques and molluscicidal activity by Adenusi, 2008. One type of glandular trichomes was found in both the adaxial and the abaxial surfaces. Young fronds were densely covered with trichomes; however, the density decreased progressively with frond maturity. Secretions flowed by diffusion through cuticle micropores, and no rupturring mechanism to release secretions was observed. Histochemical reactions revealed that the main components of the materials secreted by trichomes include polysaccharides, lipids, terpenes, phenols and flavonoids. The farina showed antifungal and molluscicidal activity. Our results strongly support an ecological role of farina as herbivore deterrent and resistance to fungi. Since N. sulphurea is a xeric adapted fern, flavonoids present in farinous secretions may also function as stress protection against harsh environmental conditions.Fil: Rodriguez, Ana María del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Derita, Marcos Gabriel. Universidad Nacional de Rosario; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Borkosky, Susana Alicia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Socolsky, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Hernández, M.A.. Fundación Miguel Lillo; ArgentinaElsevier Gmbh2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91111Rodriguez, Ana María del Valle; Derita, Marcos Gabriel; Borkosky, Susana Alicia; Socolsky, Cecilia; Bardon, Alicia del Valle; et al.; Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes; Elsevier Gmbh; Flora; 240; 3-2018; 144-1510367-2530CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.flora.2018.01.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:54:04Zoai:ri.conicet.gov.ar:11336/91111instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:54:04.706CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
title Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
spellingShingle Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
Rodriguez, Ana María del Valle
ANTIFUNGAL
FARINA
HISTOCHEMISTRY
MOLLUSCICIDAL
MORPHOLOGY
TRICHOMES
title_short Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
title_full Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
title_fullStr Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
title_full_unstemmed Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
title_sort Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes
dc.creator.none.fl_str_mv Rodriguez, Ana María del Valle
Derita, Marcos Gabriel
Borkosky, Susana Alicia
Socolsky, Cecilia
Bardon, Alicia del Valle
Hernández, M.A.
author Rodriguez, Ana María del Valle
author_facet Rodriguez, Ana María del Valle
Derita, Marcos Gabriel
Borkosky, Susana Alicia
Socolsky, Cecilia
Bardon, Alicia del Valle
Hernández, M.A.
author_role author
author2 Derita, Marcos Gabriel
Borkosky, Susana Alicia
Socolsky, Cecilia
Bardon, Alicia del Valle
Hernández, M.A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTIFUNGAL
FARINA
HISTOCHEMISTRY
MOLLUSCICIDAL
MORPHOLOGY
TRICHOMES
topic ANTIFUNGAL
FARINA
HISTOCHEMISTRY
MOLLUSCICIDAL
MORPHOLOGY
TRICHOMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The morphology and distribution of glandular trichomes in fronds at different developmental stages were investigated in Notholaena sulphurea, and the main components of the secreted material (farina) were identified. In addition antifungal and molluscicidal activities of farinose exudate were tested. Trichomes were examined using conventional light and scanning electron microscopy (SEM). The histochemical studies were conducted using the following tests on hand-sections: Toluidine Blue, Calcofluor White, Ruthenium Red, Phloroglucinol/HCl, NADI reagent, Sudan black B, Sudan IV, Nile Blue, Neutral Red, ferric chloride, Vainillin/H 2 SO 4 , Vainillin/HCl, alumimium chloride and Benedict reagent. Antifungal activity was evaluated by broth microdilution techniques and molluscicidal activity by Adenusi, 2008. One type of glandular trichomes was found in both the adaxial and the abaxial surfaces. Young fronds were densely covered with trichomes; however, the density decreased progressively with frond maturity. Secretions flowed by diffusion through cuticle micropores, and no rupturring mechanism to release secretions was observed. Histochemical reactions revealed that the main components of the materials secreted by trichomes include polysaccharides, lipids, terpenes, phenols and flavonoids. The farina showed antifungal and molluscicidal activity. Our results strongly support an ecological role of farina as herbivore deterrent and resistance to fungi. Since N. sulphurea is a xeric adapted fern, flavonoids present in farinous secretions may also function as stress protection against harsh environmental conditions.
Fil: Rodriguez, Ana María del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Derita, Marcos Gabriel. Universidad Nacional de Rosario; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Borkosky, Susana Alicia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Socolsky, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Hernández, M.A.. Fundación Miguel Lillo; Argentina
description The morphology and distribution of glandular trichomes in fronds at different developmental stages were investigated in Notholaena sulphurea, and the main components of the secreted material (farina) were identified. In addition antifungal and molluscicidal activities of farinose exudate were tested. Trichomes were examined using conventional light and scanning electron microscopy (SEM). The histochemical studies were conducted using the following tests on hand-sections: Toluidine Blue, Calcofluor White, Ruthenium Red, Phloroglucinol/HCl, NADI reagent, Sudan black B, Sudan IV, Nile Blue, Neutral Red, ferric chloride, Vainillin/H 2 SO 4 , Vainillin/HCl, alumimium chloride and Benedict reagent. Antifungal activity was evaluated by broth microdilution techniques and molluscicidal activity by Adenusi, 2008. One type of glandular trichomes was found in both the adaxial and the abaxial surfaces. Young fronds were densely covered with trichomes; however, the density decreased progressively with frond maturity. Secretions flowed by diffusion through cuticle micropores, and no rupturring mechanism to release secretions was observed. Histochemical reactions revealed that the main components of the materials secreted by trichomes include polysaccharides, lipids, terpenes, phenols and flavonoids. The farina showed antifungal and molluscicidal activity. Our results strongly support an ecological role of farina as herbivore deterrent and resistance to fungi. Since N. sulphurea is a xeric adapted fern, flavonoids present in farinous secretions may also function as stress protection against harsh environmental conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91111
Rodriguez, Ana María del Valle; Derita, Marcos Gabriel; Borkosky, Susana Alicia; Socolsky, Cecilia; Bardon, Alicia del Valle; et al.; Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes; Elsevier Gmbh; Flora; 240; 3-2018; 144-151
0367-2530
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91111
identifier_str_mv Rodriguez, Ana María del Valle; Derita, Marcos Gabriel; Borkosky, Susana Alicia; Socolsky, Cecilia; Bardon, Alicia del Valle; et al.; Bioactive farina of Notholaena sulphurea (Pteridaceae): Morphology and histochemistry of glandular trichomes; Elsevier Gmbh; Flora; 240; 3-2018; 144-151
0367-2530
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.flora.2018.01.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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