Molecular monitoring of microbial diversity in an UASB reactor

Autores
Casserly, Carolina; Erijman, Leonardo
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The dynamics of the microbial community structure of a full-scale upflow anaerobic sludge blanket reactor has been analyzed using culture-independent 16S rRNA-based methods. During the start-up of the process, denaturing gradient gel electrophoresis of bacterial rDNA showed significant changes in the structure of the bacterial community, but only a slight increase in the bacterial diversity. Associated with the shifts in bacterial populations, significant changes in the relative abundance of different methanogenic species are revealed by taxon-specific dot-blot experiments. The ratio of archaeal 16S rRNA to bacterial 16S rRNA, but none of the individual microbial taxons, correlated with the maximum allowable daily organic loading rate. The results argue against the notion that many anaerobic wastewater treatment systems fail due to the lack of adequate inoculum.
Fil: Casserly, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Anaerobic Treatment
Dairy Wastewater
16s Rrna Genes
Dgge
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79727
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Molecular monitoring of microbial diversity in an UASB reactorCasserly, CarolinaErijman, LeonardoAnaerobic TreatmentDairy Wastewater16s Rrna GenesDggehttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The dynamics of the microbial community structure of a full-scale upflow anaerobic sludge blanket reactor has been analyzed using culture-independent 16S rRNA-based methods. During the start-up of the process, denaturing gradient gel electrophoresis of bacterial rDNA showed significant changes in the structure of the bacterial community, but only a slight increase in the bacterial diversity. Associated with the shifts in bacterial populations, significant changes in the relative abundance of different methanogenic species are revealed by taxon-specific dot-blot experiments. The ratio of archaeal 16S rRNA to bacterial 16S rRNA, but none of the individual microbial taxons, correlated with the maximum allowable daily organic loading rate. The results argue against the notion that many anaerobic wastewater treatment systems fail due to the lack of adequate inoculum.Fil: Casserly, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaElsevier2003-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79727Casserly, Carolina; Erijman, Leonardo; Molecular monitoring of microbial diversity in an UASB reactor; Elsevier; International Biodeterioration and Biodegradation; 52; 1; 7-2003; 7-120964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0964-8305(02)00094-Xinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096483050200094Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:07Zoai:ri.conicet.gov.ar:11336/79727instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:07.884CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular monitoring of microbial diversity in an UASB reactor
title Molecular monitoring of microbial diversity in an UASB reactor
spellingShingle Molecular monitoring of microbial diversity in an UASB reactor
Casserly, Carolina
Anaerobic Treatment
Dairy Wastewater
16s Rrna Genes
Dgge
title_short Molecular monitoring of microbial diversity in an UASB reactor
title_full Molecular monitoring of microbial diversity in an UASB reactor
title_fullStr Molecular monitoring of microbial diversity in an UASB reactor
title_full_unstemmed Molecular monitoring of microbial diversity in an UASB reactor
title_sort Molecular monitoring of microbial diversity in an UASB reactor
dc.creator.none.fl_str_mv Casserly, Carolina
Erijman, Leonardo
author Casserly, Carolina
author_facet Casserly, Carolina
Erijman, Leonardo
author_role author
author2 Erijman, Leonardo
author2_role author
dc.subject.none.fl_str_mv Anaerobic Treatment
Dairy Wastewater
16s Rrna Genes
Dgge
topic Anaerobic Treatment
Dairy Wastewater
16s Rrna Genes
Dgge
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The dynamics of the microbial community structure of a full-scale upflow anaerobic sludge blanket reactor has been analyzed using culture-independent 16S rRNA-based methods. During the start-up of the process, denaturing gradient gel electrophoresis of bacterial rDNA showed significant changes in the structure of the bacterial community, but only a slight increase in the bacterial diversity. Associated with the shifts in bacterial populations, significant changes in the relative abundance of different methanogenic species are revealed by taxon-specific dot-blot experiments. The ratio of archaeal 16S rRNA to bacterial 16S rRNA, but none of the individual microbial taxons, correlated with the maximum allowable daily organic loading rate. The results argue against the notion that many anaerobic wastewater treatment systems fail due to the lack of adequate inoculum.
Fil: Casserly, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
description The dynamics of the microbial community structure of a full-scale upflow anaerobic sludge blanket reactor has been analyzed using culture-independent 16S rRNA-based methods. During the start-up of the process, denaturing gradient gel electrophoresis of bacterial rDNA showed significant changes in the structure of the bacterial community, but only a slight increase in the bacterial diversity. Associated with the shifts in bacterial populations, significant changes in the relative abundance of different methanogenic species are revealed by taxon-specific dot-blot experiments. The ratio of archaeal 16S rRNA to bacterial 16S rRNA, but none of the individual microbial taxons, correlated with the maximum allowable daily organic loading rate. The results argue against the notion that many anaerobic wastewater treatment systems fail due to the lack of adequate inoculum.
publishDate 2003
dc.date.none.fl_str_mv 2003-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79727
Casserly, Carolina; Erijman, Leonardo; Molecular monitoring of microbial diversity in an UASB reactor; Elsevier; International Biodeterioration and Biodegradation; 52; 1; 7-2003; 7-12
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79727
identifier_str_mv Casserly, Carolina; Erijman, Leonardo; Molecular monitoring of microbial diversity in an UASB reactor; Elsevier; International Biodeterioration and Biodegradation; 52; 1; 7-2003; 7-12
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0964-8305(02)00094-X
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096483050200094X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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