Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process
- Autores
- Moya Diaz, José Abelino; Alvarez, Yanina Daniela; Montenegro, Mauricio Norman; Bayonés, Lucas; Belingheri, Ana Verónica; González-Jamett, Arlek M.; Cárdenas, Ana M.; Marengo, Fernando Diego
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 � 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (τ = 0.53 � 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies.
Fil: Moya Diaz, José Abelino. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Alvarez, Yanina Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Montenegro, Mauricio Norman. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina
Fil: Bayonés, Lucas. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina
Fil: Belingheri, Ana Verónica. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: González-Jamett, Arlek M.. Universidad de Valparaiso; Chile
Fil: Cárdenas, Ana M.. Universidad de Valparaiso; Chile
Fil: Marengo, Fernando Diego. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina - Materia
-
CA2+ CURRENT
DYNAMIN
ENDOCYTOSIS
IMMEDIATELY RELEASABLE POOL
MEMBRANE CAPACITANCE
SECRETION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/49525
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oai:ri.conicet.gov.ar:11336/49525 |
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Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic processMoya Diaz, José AbelinoAlvarez, Yanina DanielaMontenegro, Mauricio NormanBayonés, LucasBelingheri, Ana VerónicaGonzález-Jamett, Arlek M.Cárdenas, Ana M.Marengo, Fernando DiegoCA2+ CURRENTDYNAMINENDOCYTOSISIMMEDIATELY RELEASABLE POOLMEMBRANE CAPACITANCESECRETIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 � 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (τ = 0.53 � 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies.Fil: Moya Diaz, José Abelino. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Alvarez, Yanina Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Montenegro, Mauricio Norman. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; ArgentinaFil: Bayonés, Lucas. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; ArgentinaFil: Belingheri, Ana Verónica. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: González-Jamett, Arlek M.. Universidad de Valparaiso; ChileFil: Cárdenas, Ana M.. Universidad de Valparaiso; ChileFil: Marengo, Fernando Diego. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFrontiers Media S.A.2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49525Moya Diaz, José Abelino; Alvarez, Yanina Daniela; Montenegro, Mauricio Norman; Bayonés, Lucas; Belingheri, Ana Verónica; et al.; Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process; Frontiers Media S.A.; Frontiers in Cellular Neuroscience; 10; JUL; 7-2016; 1-191662-5102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fncel.2016.00184/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2016.00184info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:55Zoai:ri.conicet.gov.ar:11336/49525instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:55.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
title |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
spellingShingle |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process Moya Diaz, José Abelino CA2+ CURRENT DYNAMIN ENDOCYTOSIS IMMEDIATELY RELEASABLE POOL MEMBRANE CAPACITANCE SECRETION |
title_short |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
title_full |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
title_fullStr |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
title_full_unstemmed |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
title_sort |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
dc.creator.none.fl_str_mv |
Moya Diaz, José Abelino Alvarez, Yanina Daniela Montenegro, Mauricio Norman Bayonés, Lucas Belingheri, Ana Verónica González-Jamett, Arlek M. Cárdenas, Ana M. Marengo, Fernando Diego |
author |
Moya Diaz, José Abelino |
author_facet |
Moya Diaz, José Abelino Alvarez, Yanina Daniela Montenegro, Mauricio Norman Bayonés, Lucas Belingheri, Ana Verónica González-Jamett, Arlek M. Cárdenas, Ana M. Marengo, Fernando Diego |
author_role |
author |
author2 |
Alvarez, Yanina Daniela Montenegro, Mauricio Norman Bayonés, Lucas Belingheri, Ana Verónica González-Jamett, Arlek M. Cárdenas, Ana M. Marengo, Fernando Diego |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
CA2+ CURRENT DYNAMIN ENDOCYTOSIS IMMEDIATELY RELEASABLE POOL MEMBRANE CAPACITANCE SECRETION |
topic |
CA2+ CURRENT DYNAMIN ENDOCYTOSIS IMMEDIATELY RELEASABLE POOL MEMBRANE CAPACITANCE SECRETION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 � 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (τ = 0.53 � 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies. Fil: Moya Diaz, José Abelino. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina Fil: Alvarez, Yanina Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina Fil: Montenegro, Mauricio Norman. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina Fil: Bayonés, Lucas. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina Fil: Belingheri, Ana Verónica. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina Fil: González-Jamett, Arlek M.. Universidad de Valparaiso; Chile Fil: Cárdenas, Ana M.. Universidad de Valparaiso; Chile Fil: Marengo, Fernando Diego. Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina |
description |
Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 � 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (τ = 0.53 � 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/49525 Moya Diaz, José Abelino; Alvarez, Yanina Daniela; Montenegro, Mauricio Norman; Bayonés, Lucas; Belingheri, Ana Verónica; et al.; Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process; Frontiers Media S.A.; Frontiers in Cellular Neuroscience; 10; JUL; 7-2016; 1-19 1662-5102 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/49525 |
identifier_str_mv |
Moya Diaz, José Abelino; Alvarez, Yanina Daniela; Montenegro, Mauricio Norman; Bayonés, Lucas; Belingheri, Ana Verónica; et al.; Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process; Frontiers Media S.A.; Frontiers in Cellular Neuroscience; 10; JUL; 7-2016; 1-19 1662-5102 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fncel.2016.00184/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2016.00184 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613839775072256 |
score |
13.070432 |