Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal

Autores
Mazzei, Luciana Jorgelina; García, Isabel Mercedes; Manucha, Walter Ariel Fernando
Año de publicación
2010
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wilms tumor gene 1 (wt-1), a key regulator of mesenchymal-epithelial transformation, is  downregulated during congenital obstructive nephropathy, leading to apoptosis. There is a functional interaction  between WT-1 and inducible nitric oxide synthase (iNOS). In this regard, we reported that after neonatal  unilateral ureteral obstruction, rosuvastatin prevents apoptosis through an increase in nitric oxide bioavailability,  which in turn is linked to higher Hsp70 expression. Hence, the goal of this study was to determine whether a  nitric oxide/Hsp70 interaction is involved in changes in WT-1 mRNA expression after ureteral obstruction.  Neonatal rats submitted to experimental ureteral obstruction were treated with either vehicle or rosuvastatin  for 14 days. Decreased nitric oxide and iNOS/Hsp70 expression associated with WT-1 low expression was  shown in obstructed kidneys. Apoptosis was induced and it was associated with an increased Bax/BcL2 ratio.  Conversely, iNOS/Hsp70 upregulation and an increased WT-1 mRNA expression, without an apoptotic response,  were observed in the cortex of obstructed kidneys of rosuvastatin-treated rats. Nitric oxide also modulated  Hsp70 and WT-1 mRNA expression in MDCK cells. Finally, in vivo experiments with nitric oxide  modulators support our hypothesis that WT-1 mRNA expression is associated with nitric oxide level. Results  suggest that rosuvastatin may modulate WT-1 mRNA expression through renal nitric oxide bioavailability,  preventing neonatal obstruction-induced apoptosis associated with Hsp70 interaction.
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina
Fil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Manucha, Walter Ariel Fernando. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
Obstructive Nephropathy
Apoptosis
Statins
Kidney Development
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81258
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/81258
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatalMazzei, Luciana JorgelinaGarcía, Isabel MercedesManucha, Walter Ariel FernandoObstructive NephropathyApoptosisStatinsKidney Developmenthttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Wilms tumor gene 1 (wt-1), a key regulator of mesenchymal-epithelial transformation, is  downregulated during congenital obstructive nephropathy, leading to apoptosis. There is a functional interaction  between WT-1 and inducible nitric oxide synthase (iNOS). In this regard, we reported that after neonatal  unilateral ureteral obstruction, rosuvastatin prevents apoptosis through an increase in nitric oxide bioavailability,  which in turn is linked to higher Hsp70 expression. Hence, the goal of this study was to determine whether a  nitric oxide/Hsp70 interaction is involved in changes in WT-1 mRNA expression after ureteral obstruction.  Neonatal rats submitted to experimental ureteral obstruction were treated with either vehicle or rosuvastatin  for 14 days. Decreased nitric oxide and iNOS/Hsp70 expression associated with WT-1 low expression was  shown in obstructed kidneys. Apoptosis was induced and it was associated with an increased Bax/BcL2 ratio.  Conversely, iNOS/Hsp70 upregulation and an increased WT-1 mRNA expression, without an apoptotic response,  were observed in the cortex of obstructed kidneys of rosuvastatin-treated rats. Nitric oxide also modulated  Hsp70 and WT-1 mRNA expression in MDCK cells. Finally, in vivo experiments with nitric oxide  modulators support our hypothesis that WT-1 mRNA expression is associated with nitric oxide level. Results  suggest that rosuvastatin may modulate WT-1 mRNA expression through renal nitric oxide bioavailability,  preventing neonatal obstruction-induced apoptosis associated with Hsp70 interaction.Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; ArgentinaFil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Manucha, Walter Ariel Fernando. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaGrupo Bio SRL2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81258Mazzei, Luciana Jorgelina; García, Isabel Mercedes; Manucha, Walter Ariel Fernando; Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal; Grupo Bio SRL; Revista Bioanálisis; 6; 6-2010; 20-261669-8703CONICET DigitalCONICETspainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:47Zoai:ri.conicet.gov.ar:11336/81258instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:47.31CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
title Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
spellingShingle Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
Mazzei, Luciana Jorgelina
Obstructive Nephropathy
Apoptosis
Statins
Kidney Development
title_short Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
title_full Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
title_fullStr Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
title_full_unstemmed Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
title_sort Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal
dc.creator.none.fl_str_mv Mazzei, Luciana Jorgelina
García, Isabel Mercedes
Manucha, Walter Ariel Fernando
author Mazzei, Luciana Jorgelina
author_facet Mazzei, Luciana Jorgelina
García, Isabel Mercedes
Manucha, Walter Ariel Fernando
author_role author
author2 García, Isabel Mercedes
Manucha, Walter Ariel Fernando
author2_role author
author
dc.subject.none.fl_str_mv Obstructive Nephropathy
Apoptosis
Statins
Kidney Development
topic Obstructive Nephropathy
Apoptosis
Statins
Kidney Development
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Wilms tumor gene 1 (wt-1), a key regulator of mesenchymal-epithelial transformation, is  downregulated during congenital obstructive nephropathy, leading to apoptosis. There is a functional interaction  between WT-1 and inducible nitric oxide synthase (iNOS). In this regard, we reported that after neonatal  unilateral ureteral obstruction, rosuvastatin prevents apoptosis through an increase in nitric oxide bioavailability,  which in turn is linked to higher Hsp70 expression. Hence, the goal of this study was to determine whether a  nitric oxide/Hsp70 interaction is involved in changes in WT-1 mRNA expression after ureteral obstruction.  Neonatal rats submitted to experimental ureteral obstruction were treated with either vehicle or rosuvastatin  for 14 days. Decreased nitric oxide and iNOS/Hsp70 expression associated with WT-1 low expression was  shown in obstructed kidneys. Apoptosis was induced and it was associated with an increased Bax/BcL2 ratio.  Conversely, iNOS/Hsp70 upregulation and an increased WT-1 mRNA expression, without an apoptotic response,  were observed in the cortex of obstructed kidneys of rosuvastatin-treated rats. Nitric oxide also modulated  Hsp70 and WT-1 mRNA expression in MDCK cells. Finally, in vivo experiments with nitric oxide  modulators support our hypothesis that WT-1 mRNA expression is associated with nitric oxide level. Results  suggest that rosuvastatin may modulate WT-1 mRNA expression through renal nitric oxide bioavailability,  preventing neonatal obstruction-induced apoptosis associated with Hsp70 interaction.
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina
Fil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Manucha, Walter Ariel Fernando. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Wilms tumor gene 1 (wt-1), a key regulator of mesenchymal-epithelial transformation, is  downregulated during congenital obstructive nephropathy, leading to apoptosis. There is a functional interaction  between WT-1 and inducible nitric oxide synthase (iNOS). In this regard, we reported that after neonatal  unilateral ureteral obstruction, rosuvastatin prevents apoptosis through an increase in nitric oxide bioavailability,  which in turn is linked to higher Hsp70 expression. Hence, the goal of this study was to determine whether a  nitric oxide/Hsp70 interaction is involved in changes in WT-1 mRNA expression after ureteral obstruction.  Neonatal rats submitted to experimental ureteral obstruction were treated with either vehicle or rosuvastatin  for 14 days. Decreased nitric oxide and iNOS/Hsp70 expression associated with WT-1 low expression was  shown in obstructed kidneys. Apoptosis was induced and it was associated with an increased Bax/BcL2 ratio.  Conversely, iNOS/Hsp70 upregulation and an increased WT-1 mRNA expression, without an apoptotic response,  were observed in the cortex of obstructed kidneys of rosuvastatin-treated rats. Nitric oxide also modulated  Hsp70 and WT-1 mRNA expression in MDCK cells. Finally, in vivo experiments with nitric oxide  modulators support our hypothesis that WT-1 mRNA expression is associated with nitric oxide level. Results  suggest that rosuvastatin may modulate WT-1 mRNA expression through renal nitric oxide bioavailability,  preventing neonatal obstruction-induced apoptosis associated with Hsp70 interaction.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81258
Mazzei, Luciana Jorgelina; García, Isabel Mercedes; Manucha, Walter Ariel Fernando; Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal; Grupo Bio SRL; Revista Bioanálisis; 6; 6-2010; 20-26
1669-8703
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81258
identifier_str_mv Mazzei, Luciana Jorgelina; García, Isabel Mercedes; Manucha, Walter Ariel Fernando; Moduladores de fibrosis y apoptosis asociados a la disponibilidad de on: Efecto de rosuvastanina en nefropatia obstructiva neonatal; Grupo Bio SRL; Revista Bioanálisis; 6; 6-2010; 20-26
1669-8703
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Grupo Bio SRL
publisher.none.fl_str_mv Grupo Bio SRL
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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