Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals

Autores
Durando, Milena de Lourdes; Canessini Guillermina; Cocito, Laura Leilén; Galoppo, Germán Hugo; Zayas, Marcelo Alejandro; Luque, Enrique Hugo; Muñoz de Toro, Monica Milagros
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Studies regarding the effects of endocrine-disrupting chemicals (EDCs) on the reproductivefunctions of wild animals have raised increasing concern. Thus, here we evaluated theconsequences of in ovo exposure to endosulfan (END) and bisphenol A (BPA) in testes fromneonatal to juvenile (Juv) caimans (Caiman latirostris). Caiman eggs were collected from areaswith low to moderate anthropogenic intervention and incubated at male-producing temperature.At stage 20 of embryonic development (previous to gonad sex determination), eggs were exposedto either END (20 ppm) or BPA (1.4 ppm) and male gonad histomorphology examined in 10-day-old, 90-day-old, and Juv caimans. The relative seminiferous tubular area (RTA) was measuredin testes and the proliferation index and the expression of estrogen receptor alpha (ERa) werequantified in intratubular cells. Regardless of the treatment, all eggs resulted in male hatchlings.The testes of EDC-exposed caimans presented tortuous seminiferous tubules with empty tubularlumens. The RTA of 10-day-old caimans exposed to BPA was decreased. The percentage of cellsexpressing ERa was not different after in ovo treatment with EDCs (compared to the Controlgroup), although caimans exposed to END showed a different ERa distribution pattern. Theproliferation index was lower in 90-day-old caimans exposed to END, and higher in Juv caimansexposed to BPA. In ovo exposure to END or BPA modified sensitive parameters of C. latirostris malegonads. The alterations described here might compromise not only the sexual maturation but alsothe reproductive performance of adult caimans.
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Canessini Guillermina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Cocito, Laura Leilén. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Galoppo, Germán Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Zayas, Marcelo Alejandro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Materia
BISPHENOL A
ENDOSULFAN
TESTES
REPTILES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/110278
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/110278
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicalsDurando, Milena de LourdesCanessini GuillerminaCocito, Laura LeilénGaloppo, Germán HugoZayas, Marcelo AlejandroLuque, Enrique HugoMuñoz de Toro, Monica MilagrosBISPHENOL AENDOSULFANTESTESREPTILEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Studies regarding the effects of endocrine-disrupting chemicals (EDCs) on the reproductivefunctions of wild animals have raised increasing concern. Thus, here we evaluated theconsequences of in ovo exposure to endosulfan (END) and bisphenol A (BPA) in testes fromneonatal to juvenile (Juv) caimans (Caiman latirostris). Caiman eggs were collected from areaswith low to moderate anthropogenic intervention and incubated at male-producing temperature.At stage 20 of embryonic development (previous to gonad sex determination), eggs were exposedto either END (20 ppm) or BPA (1.4 ppm) and male gonad histomorphology examined in 10-day-old, 90-day-old, and Juv caimans. The relative seminiferous tubular area (RTA) was measuredin testes and the proliferation index and the expression of estrogen receptor alpha (ERa) werequantified in intratubular cells. Regardless of the treatment, all eggs resulted in male hatchlings.The testes of EDC-exposed caimans presented tortuous seminiferous tubules with empty tubularlumens. The RTA of 10-day-old caimans exposed to BPA was decreased. The percentage of cellsexpressing ERa was not different after in ovo treatment with EDCs (compared to the Controlgroup), although caimans exposed to END showed a different ERa distribution pattern. Theproliferation index was lower in 90-day-old caimans exposed to END, and higher in Juv caimansexposed to BPA. In ovo exposure to END or BPA modified sensitive parameters of C. latirostris malegonads. The alterations described here might compromise not only the sexual maturation but alsothe reproductive performance of adult caimans.Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaFil: Canessini Guillermina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaFil: Cocito, Laura Leilén. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaFil: Galoppo, Germán Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaFil: Zayas, Marcelo Alejandro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaFil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaFil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaVeterinary and Human Toxicology2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110278Durando, Milena de Lourdes; Canessini Guillermina; Cocito, Laura Leilén; Galoppo, Germán Hugo; Zayas, Marcelo Alejandro; et al.; Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals; Veterinary and Human Toxicology; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 325; 1-2016; 84-961932-5223CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1999info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:35:29Zoai:ri.conicet.gov.ar:11336/110278instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:35:29.831CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
title Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
spellingShingle Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
Durando, Milena de Lourdes
BISPHENOL A
ENDOSULFAN
TESTES
REPTILES
title_short Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
title_full Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
title_fullStr Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
title_full_unstemmed Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
title_sort Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals
dc.creator.none.fl_str_mv Durando, Milena de Lourdes
Canessini Guillermina
Cocito, Laura Leilén
Galoppo, Germán Hugo
Zayas, Marcelo Alejandro
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author Durando, Milena de Lourdes
author_facet Durando, Milena de Lourdes
Canessini Guillermina
Cocito, Laura Leilén
Galoppo, Germán Hugo
Zayas, Marcelo Alejandro
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author_role author
author2 Canessini Guillermina
Cocito, Laura Leilén
Galoppo, Germán Hugo
Zayas, Marcelo Alejandro
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BISPHENOL A
ENDOSULFAN
TESTES
REPTILES
topic BISPHENOL A
ENDOSULFAN
TESTES
REPTILES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Studies regarding the effects of endocrine-disrupting chemicals (EDCs) on the reproductivefunctions of wild animals have raised increasing concern. Thus, here we evaluated theconsequences of in ovo exposure to endosulfan (END) and bisphenol A (BPA) in testes fromneonatal to juvenile (Juv) caimans (Caiman latirostris). Caiman eggs were collected from areaswith low to moderate anthropogenic intervention and incubated at male-producing temperature.At stage 20 of embryonic development (previous to gonad sex determination), eggs were exposedto either END (20 ppm) or BPA (1.4 ppm) and male gonad histomorphology examined in 10-day-old, 90-day-old, and Juv caimans. The relative seminiferous tubular area (RTA) was measuredin testes and the proliferation index and the expression of estrogen receptor alpha (ERa) werequantified in intratubular cells. Regardless of the treatment, all eggs resulted in male hatchlings.The testes of EDC-exposed caimans presented tortuous seminiferous tubules with empty tubularlumens. The RTA of 10-day-old caimans exposed to BPA was decreased. The percentage of cellsexpressing ERa was not different after in ovo treatment with EDCs (compared to the Controlgroup), although caimans exposed to END showed a different ERa distribution pattern. Theproliferation index was lower in 90-day-old caimans exposed to END, and higher in Juv caimansexposed to BPA. In ovo exposure to END or BPA modified sensitive parameters of C. latirostris malegonads. The alterations described here might compromise not only the sexual maturation but alsothe reproductive performance of adult caimans.
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Canessini Guillermina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Cocito, Laura Leilén. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Galoppo, Germán Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Zayas, Marcelo Alejandro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
description Studies regarding the effects of endocrine-disrupting chemicals (EDCs) on the reproductivefunctions of wild animals have raised increasing concern. Thus, here we evaluated theconsequences of in ovo exposure to endosulfan (END) and bisphenol A (BPA) in testes fromneonatal to juvenile (Juv) caimans (Caiman latirostris). Caiman eggs were collected from areaswith low to moderate anthropogenic intervention and incubated at male-producing temperature.At stage 20 of embryonic development (previous to gonad sex determination), eggs were exposedto either END (20 ppm) or BPA (1.4 ppm) and male gonad histomorphology examined in 10-day-old, 90-day-old, and Juv caimans. The relative seminiferous tubular area (RTA) was measuredin testes and the proliferation index and the expression of estrogen receptor alpha (ERa) werequantified in intratubular cells. Regardless of the treatment, all eggs resulted in male hatchlings.The testes of EDC-exposed caimans presented tortuous seminiferous tubules with empty tubularlumens. The RTA of 10-day-old caimans exposed to BPA was decreased. The percentage of cellsexpressing ERa was not different after in ovo treatment with EDCs (compared to the Controlgroup), although caimans exposed to END showed a different ERa distribution pattern. Theproliferation index was lower in 90-day-old caimans exposed to END, and higher in Juv caimansexposed to BPA. In ovo exposure to END or BPA modified sensitive parameters of C. latirostris malegonads. The alterations described here might compromise not only the sexual maturation but alsothe reproductive performance of adult caimans.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/110278
Durando, Milena de Lourdes; Canessini Guillermina; Cocito, Laura Leilén; Galoppo, Germán Hugo; Zayas, Marcelo Alejandro; et al.; Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals; Veterinary and Human Toxicology; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 325; 1-2016; 84-96
1932-5223
CONICET Digital
CONICET
url http://hdl.handle.net/11336/110278
identifier_str_mv Durando, Milena de Lourdes; Canessini Guillermina; Cocito, Laura Leilén; Galoppo, Germán Hugo; Zayas, Marcelo Alejandro; et al.; Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals; Veterinary and Human Toxicology; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 325; 1-2016; 84-96
1932-5223
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1999
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Veterinary and Human Toxicology
publisher.none.fl_str_mv Veterinary and Human Toxicology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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