Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals

Autores
Durando, Milena de Lourdes; Cocito, Laura; Rodriguez, Horacio Adolfo; Varayoud, Jorgelina Guadalupe; Ramos, Jorge G.; Luque, Enrique Hugo; Muñoz de Toro, Mónica
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Caiman latirostris is one of the reptilian species that exhibits temperature-dependent sex determination (TSD). In addition, male-to-female sex reversal after in ovo estrogen/xenoestrogen exposure was demonstrated, called as hormone-dependent sex determination (HSD). The amh, sox9 and sf-1 genes are involved in sex determination, sex differentiation, and steroidogenesis. The aims of this study were: a) to establish the expression patterns of amh, sox9 and sf-1 mRNA in the gonad-adrenal-mesonephros (GAM) complexes of neonatal TSD-male and TSD-female caimans, b) to compare the expression of these genes between TSD-females versus HSD-females (born from E2-exposed eggs incubated at the male-producing temperature) and c) to evaluate whether these genes are affected by in ovo exposure to 17b-estradiol (E2) and two endocrine disrupting chemicals (bisphenol A (BPA) or endosulfan (END)). The mRNA expression of amh, sox9 and sf-1 in GAM complexes from TSD-males and TSD-females, and from HSD-females were quantitatively compared by RT-PCR. In neonatal caimans, we demonstrated a sexually dimorphic pattern of amh and sox9 mRNA expression, with a higher expression in TSD-male than in TSD-female GAM. sf-1 mRNA did not differ between TSD-males and TSD-females. Between females, HSD-females exhibited a higher expression of sox9 than TSD-females. Increased mRNA expression of sex-determining genes was observed in the GAM of neonatal males, in ovo exposed to END. No changes were observed after BPA exposure, and E2 decreased the sox9 but increased the sf-1 mRNA expression. These results provide a new insight into the mechanisms of gonadal histo-functional alterations in caimans exposed to contaminated environments.
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Cocito, Laura. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Rodriguez, Horacio Adolfo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Varayoud, Jorgelina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Ramos, Jorge G. . Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Muñoz de Toro, Mónica. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Materia
Caiman Latirostris
Development
Endocrinology
Sox
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6404
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicalsDurando, Milena de LourdesCocito, LauraRodriguez, Horacio AdolfoVarayoud, Jorgelina GuadalupeRamos, Jorge G. Luque, Enrique HugoMuñoz de Toro, MónicaCaiman LatirostrisDevelopmentEndocrinologySoxhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Caiman latirostris is one of the reptilian species that exhibits temperature-dependent sex determination (TSD). In addition, male-to-female sex reversal after in ovo estrogen/xenoestrogen exposure was demonstrated, called as hormone-dependent sex determination (HSD). The amh, sox9 and sf-1 genes are involved in sex determination, sex differentiation, and steroidogenesis. The aims of this study were: a) to establish the expression patterns of amh, sox9 and sf-1 mRNA in the gonad-adrenal-mesonephros (GAM) complexes of neonatal TSD-male and TSD-female caimans, b) to compare the expression of these genes between TSD-females versus HSD-females (born from E2-exposed eggs incubated at the male-producing temperature) and c) to evaluate whether these genes are affected by in ovo exposure to 17b-estradiol (E2) and two endocrine disrupting chemicals (bisphenol A (BPA) or endosulfan (END)). The mRNA expression of amh, sox9 and sf-1 in GAM complexes from TSD-males and TSD-females, and from HSD-females were quantitatively compared by RT-PCR. In neonatal caimans, we demonstrated a sexually dimorphic pattern of amh and sox9 mRNA expression, with a higher expression in TSD-male than in TSD-female GAM. sf-1 mRNA did not differ between TSD-males and TSD-females. Between females, HSD-females exhibited a higher expression of sox9 than TSD-females. Increased mRNA expression of sex-determining genes was observed in the GAM of neonatal males, in ovo exposed to END. No changes were observed after BPA exposure, and E2 decreased the sox9 but increased the sf-1 mRNA expression. These results provide a new insight into the mechanisms of gonadal histo-functional alterations in caimans exposed to contaminated environments.Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Cocito, Laura. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Rodriguez, Horacio Adolfo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Varayoud, Jorgelina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Ramos, Jorge G. . Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Muñoz de Toro, Mónica. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaElsevier2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6404Durando, Milena de Lourdes; Cocito, Laura; Rodriguez, Horacio Adolfo; Varayoud, Jorgelina Guadalupe; Ramos, Jorge G. ; et al.; Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals; Elsevier; General and Comparative Endocrinology; 191; 6-2013; 31-380016-6480enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0016648013002359info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2013.05.013info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:36Zoai:ri.conicet.gov.ar:11336/6404instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:36.559CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
title Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
spellingShingle Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
Durando, Milena de Lourdes
Caiman Latirostris
Development
Endocrinology
Sox
title_short Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
title_full Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
title_fullStr Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
title_full_unstemmed Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
title_sort Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals
dc.creator.none.fl_str_mv Durando, Milena de Lourdes
Cocito, Laura
Rodriguez, Horacio Adolfo
Varayoud, Jorgelina Guadalupe
Ramos, Jorge G.
Luque, Enrique Hugo
Muñoz de Toro, Mónica
author Durando, Milena de Lourdes
author_facet Durando, Milena de Lourdes
Cocito, Laura
Rodriguez, Horacio Adolfo
Varayoud, Jorgelina Guadalupe
Ramos, Jorge G.
Luque, Enrique Hugo
Muñoz de Toro, Mónica
author_role author
author2 Cocito, Laura
Rodriguez, Horacio Adolfo
Varayoud, Jorgelina Guadalupe
Ramos, Jorge G.
Luque, Enrique Hugo
Muñoz de Toro, Mónica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Caiman Latirostris
Development
Endocrinology
Sox
topic Caiman Latirostris
Development
Endocrinology
Sox
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Caiman latirostris is one of the reptilian species that exhibits temperature-dependent sex determination (TSD). In addition, male-to-female sex reversal after in ovo estrogen/xenoestrogen exposure was demonstrated, called as hormone-dependent sex determination (HSD). The amh, sox9 and sf-1 genes are involved in sex determination, sex differentiation, and steroidogenesis. The aims of this study were: a) to establish the expression patterns of amh, sox9 and sf-1 mRNA in the gonad-adrenal-mesonephros (GAM) complexes of neonatal TSD-male and TSD-female caimans, b) to compare the expression of these genes between TSD-females versus HSD-females (born from E2-exposed eggs incubated at the male-producing temperature) and c) to evaluate whether these genes are affected by in ovo exposure to 17b-estradiol (E2) and two endocrine disrupting chemicals (bisphenol A (BPA) or endosulfan (END)). The mRNA expression of amh, sox9 and sf-1 in GAM complexes from TSD-males and TSD-females, and from HSD-females were quantitatively compared by RT-PCR. In neonatal caimans, we demonstrated a sexually dimorphic pattern of amh and sox9 mRNA expression, with a higher expression in TSD-male than in TSD-female GAM. sf-1 mRNA did not differ between TSD-males and TSD-females. Between females, HSD-females exhibited a higher expression of sox9 than TSD-females. Increased mRNA expression of sex-determining genes was observed in the GAM of neonatal males, in ovo exposed to END. No changes were observed after BPA exposure, and E2 decreased the sox9 but increased the sf-1 mRNA expression. These results provide a new insight into the mechanisms of gonadal histo-functional alterations in caimans exposed to contaminated environments.
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Cocito, Laura. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Rodriguez, Horacio Adolfo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Varayoud, Jorgelina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Ramos, Jorge G. . Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Muñoz de Toro, Mónica. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
description Caiman latirostris is one of the reptilian species that exhibits temperature-dependent sex determination (TSD). In addition, male-to-female sex reversal after in ovo estrogen/xenoestrogen exposure was demonstrated, called as hormone-dependent sex determination (HSD). The amh, sox9 and sf-1 genes are involved in sex determination, sex differentiation, and steroidogenesis. The aims of this study were: a) to establish the expression patterns of amh, sox9 and sf-1 mRNA in the gonad-adrenal-mesonephros (GAM) complexes of neonatal TSD-male and TSD-female caimans, b) to compare the expression of these genes between TSD-females versus HSD-females (born from E2-exposed eggs incubated at the male-producing temperature) and c) to evaluate whether these genes are affected by in ovo exposure to 17b-estradiol (E2) and two endocrine disrupting chemicals (bisphenol A (BPA) or endosulfan (END)). The mRNA expression of amh, sox9 and sf-1 in GAM complexes from TSD-males and TSD-females, and from HSD-females were quantitatively compared by RT-PCR. In neonatal caimans, we demonstrated a sexually dimorphic pattern of amh and sox9 mRNA expression, with a higher expression in TSD-male than in TSD-female GAM. sf-1 mRNA did not differ between TSD-males and TSD-females. Between females, HSD-females exhibited a higher expression of sox9 than TSD-females. Increased mRNA expression of sex-determining genes was observed in the GAM of neonatal males, in ovo exposed to END. No changes were observed after BPA exposure, and E2 decreased the sox9 but increased the sf-1 mRNA expression. These results provide a new insight into the mechanisms of gonadal histo-functional alterations in caimans exposed to contaminated environments.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6404
Durando, Milena de Lourdes; Cocito, Laura; Rodriguez, Horacio Adolfo; Varayoud, Jorgelina Guadalupe; Ramos, Jorge G. ; et al.; Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals; Elsevier; General and Comparative Endocrinology; 191; 6-2013; 31-38
0016-6480
url http://hdl.handle.net/11336/6404
identifier_str_mv Durando, Milena de Lourdes; Cocito, Laura; Rodriguez, Horacio Adolfo; Varayoud, Jorgelina Guadalupe; Ramos, Jorge G. ; et al.; Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals; Elsevier; General and Comparative Endocrinology; 191; 6-2013; 31-38
0016-6480
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0016648013002359
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2013.05.013
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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