Potential involvement of C3 complement factor in amphibian fertilization
- Autores
- Llanos, Ricardo Javier; Mansilla Whitacre, Cecilia; Miceli, Dora Cristina
- Año de publicación
- 2000
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We have assessed the potential involvement of C3, the third complement factor, and its receptor in Bufo arenarum fertilization. We show that a polyclonal antibody against a B. arenarum C3-like factor (C3Ba) reacts specifically with components of the extracellular matrix (ECM) of coelomic eggs and the cell membrane of uterine eggs. Interestingly, we have identified a 163 kD protein immunoreactive with a monoclonal antibody against the CD11b α chain of the human C3 receptor on the cell membrane of the animal pole of uterine eggs, the site of entry of the sperm, but not in coelomic eggs (CR3Ba). Treatment of coelomic eggs with a pars recta oviductal-like protease, trypsin, induced the translocation of C3Ba from the ECM to the cell membrane. Furthermore, inhibition of CR3Ba by trypan blue, as well as inhibition of C3Ba by anti-C3Ba on uterine eggs impaired fertilization, whereas identical treatment on sperm cells did not alter percentage fertilization. Our results suggest, (A) that changes in the localization of C3Ba from the ECM to the cell membrane may be triggered by trypsin-like proteases during passage of eggs through the oviduct; and (B) that C3Ba/CR3Ba may be involved in B. arenarum fertilization.
Fil: Llanos, Ricardo Javier. Universidad Nacional de Tucumán; Argentina
Fil: Mansilla Whitacre, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Miceli, Dora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina - Materia
-
BUFO ARENARUM
COMPLEMENT
FERTILIZATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/97246
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Potential involvement of C3 complement factor in amphibian fertilizationLlanos, Ricardo JavierMansilla Whitacre, CeciliaMiceli, Dora CristinaBUFO ARENARUMCOMPLEMENTFERTILIZATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have assessed the potential involvement of C3, the third complement factor, and its receptor in Bufo arenarum fertilization. We show that a polyclonal antibody against a B. arenarum C3-like factor (C3Ba) reacts specifically with components of the extracellular matrix (ECM) of coelomic eggs and the cell membrane of uterine eggs. Interestingly, we have identified a 163 kD protein immunoreactive with a monoclonal antibody against the CD11b α chain of the human C3 receptor on the cell membrane of the animal pole of uterine eggs, the site of entry of the sperm, but not in coelomic eggs (CR3Ba). Treatment of coelomic eggs with a pars recta oviductal-like protease, trypsin, induced the translocation of C3Ba from the ECM to the cell membrane. Furthermore, inhibition of CR3Ba by trypan blue, as well as inhibition of C3Ba by anti-C3Ba on uterine eggs impaired fertilization, whereas identical treatment on sperm cells did not alter percentage fertilization. Our results suggest, (A) that changes in the localization of C3Ba from the ECM to the cell membrane may be triggered by trypsin-like proteases during passage of eggs through the oviduct; and (B) that C3Ba/CR3Ba may be involved in B. arenarum fertilization.Fil: Llanos, Ricardo Javier. Universidad Nacional de Tucumán; ArgentinaFil: Mansilla Whitacre, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Miceli, Dora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier Science Inc2000-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97246Llanos, Ricardo Javier; Mansilla Whitacre, Cecilia; Miceli, Dora Cristina; Potential involvement of C3 complement factor in amphibian fertilization; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 127; 1; 9-2000; 29-381095-6433CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S1095-6433(00)00228-2info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1095643300002282info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:44Zoai:ri.conicet.gov.ar:11336/97246instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:44.36CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Potential involvement of C3 complement factor in amphibian fertilization |
title |
Potential involvement of C3 complement factor in amphibian fertilization |
spellingShingle |
Potential involvement of C3 complement factor in amphibian fertilization Llanos, Ricardo Javier BUFO ARENARUM COMPLEMENT FERTILIZATION |
title_short |
Potential involvement of C3 complement factor in amphibian fertilization |
title_full |
Potential involvement of C3 complement factor in amphibian fertilization |
title_fullStr |
Potential involvement of C3 complement factor in amphibian fertilization |
title_full_unstemmed |
Potential involvement of C3 complement factor in amphibian fertilization |
title_sort |
Potential involvement of C3 complement factor in amphibian fertilization |
dc.creator.none.fl_str_mv |
Llanos, Ricardo Javier Mansilla Whitacre, Cecilia Miceli, Dora Cristina |
author |
Llanos, Ricardo Javier |
author_facet |
Llanos, Ricardo Javier Mansilla Whitacre, Cecilia Miceli, Dora Cristina |
author_role |
author |
author2 |
Mansilla Whitacre, Cecilia Miceli, Dora Cristina |
author2_role |
author author |
dc.subject.none.fl_str_mv |
BUFO ARENARUM COMPLEMENT FERTILIZATION |
topic |
BUFO ARENARUM COMPLEMENT FERTILIZATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We have assessed the potential involvement of C3, the third complement factor, and its receptor in Bufo arenarum fertilization. We show that a polyclonal antibody against a B. arenarum C3-like factor (C3Ba) reacts specifically with components of the extracellular matrix (ECM) of coelomic eggs and the cell membrane of uterine eggs. Interestingly, we have identified a 163 kD protein immunoreactive with a monoclonal antibody against the CD11b α chain of the human C3 receptor on the cell membrane of the animal pole of uterine eggs, the site of entry of the sperm, but not in coelomic eggs (CR3Ba). Treatment of coelomic eggs with a pars recta oviductal-like protease, trypsin, induced the translocation of C3Ba from the ECM to the cell membrane. Furthermore, inhibition of CR3Ba by trypan blue, as well as inhibition of C3Ba by anti-C3Ba on uterine eggs impaired fertilization, whereas identical treatment on sperm cells did not alter percentage fertilization. Our results suggest, (A) that changes in the localization of C3Ba from the ECM to the cell membrane may be triggered by trypsin-like proteases during passage of eggs through the oviduct; and (B) that C3Ba/CR3Ba may be involved in B. arenarum fertilization. Fil: Llanos, Ricardo Javier. Universidad Nacional de Tucumán; Argentina Fil: Mansilla Whitacre, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Miceli, Dora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina |
description |
We have assessed the potential involvement of C3, the third complement factor, and its receptor in Bufo arenarum fertilization. We show that a polyclonal antibody against a B. arenarum C3-like factor (C3Ba) reacts specifically with components of the extracellular matrix (ECM) of coelomic eggs and the cell membrane of uterine eggs. Interestingly, we have identified a 163 kD protein immunoreactive with a monoclonal antibody against the CD11b α chain of the human C3 receptor on the cell membrane of the animal pole of uterine eggs, the site of entry of the sperm, but not in coelomic eggs (CR3Ba). Treatment of coelomic eggs with a pars recta oviductal-like protease, trypsin, induced the translocation of C3Ba from the ECM to the cell membrane. Furthermore, inhibition of CR3Ba by trypan blue, as well as inhibition of C3Ba by anti-C3Ba on uterine eggs impaired fertilization, whereas identical treatment on sperm cells did not alter percentage fertilization. Our results suggest, (A) that changes in the localization of C3Ba from the ECM to the cell membrane may be triggered by trypsin-like proteases during passage of eggs through the oviduct; and (B) that C3Ba/CR3Ba may be involved in B. arenarum fertilization. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/97246 Llanos, Ricardo Javier; Mansilla Whitacre, Cecilia; Miceli, Dora Cristina; Potential involvement of C3 complement factor in amphibian fertilization; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 127; 1; 9-2000; 29-38 1095-6433 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/97246 |
identifier_str_mv |
Llanos, Ricardo Javier; Mansilla Whitacre, Cecilia; Miceli, Dora Cristina; Potential involvement of C3 complement factor in amphibian fertilization; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 127; 1; 9-2000; 29-38 1095-6433 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/S1095-6433(00)00228-2 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1095643300002282 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Inc |
publisher.none.fl_str_mv |
Elsevier Science Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614497547845632 |
score |
13.070432 |