SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis...

Autores
Trupkin, Santiago Ariel; Auge, Gabriela Alejandra; Zhu, Jian-Kang; Sanchez, Rodolfo Augusto; Botto, Javier Francisco
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Premise of research. Plants cope with complex environments throughout their life cycles. The perception and integration of information acquired from different environmental parameters are key to responding appropriately and securing plant survival. In Arabidopsis thaliana, SALT OVERLY SENSITIVE 2 (SOS2) and SOS3, its protein-interacting partner, have been described as central players in salt-stress responses, while another SOS2-interacting partner, ABSCISIC ACID (ABA)–INSENSITIVE 2 (ABI2), is involved in ABA signaling. SOS2 was also suggested to be involved in the photocontrol of seed germination, although its function in photomorphogenesis is not fully understood. Here we studied the role played by SOS2, SOS3, and ABI2 in light-dependent responses in Arabidopsis mutants. We assessed whether SOS2, SOS3, and ABI2 modulate light- and hormone-regulated seed germination and seedling deetiolation. Methodology. We examined physiological responses in wild-type and SOS2, SOS3, and ABI2 knockout mutants of A. thaliana exposed to red light and explored whether these proteins modulate germination (by changing hormone sensitivity) and seedling deetiolation. In addition, we analyzed the presence of cis-regulatory elements (CREs) in their promoters and expression profiles from public microarrays to confirm their function in photomorphogenesis. Pivotal results. Germination experiments demonstrate that SOS2, SOS3, and ABI2 are positive regulators of germination induced by red light. Differences in germination between the wild type and mutants are explained by changes in gibberellin sensitivity. Analysis of hypocotyl growth inhibition and cotyledon opening suggest that these components are also required for full seedling deetiolation under red light. Furthermore, identification of CREs in gene promoters and expression analysis matched the proposed role for these genes in both processes. Conclusions. SOS2, SOS3, and ABI2 are known components of salt signaling, and here we demonstrate that they are also positive elements of light signaling by regulating seedling deetiolation and altering gibberellic acid sensitivity during germination. These results suggest that they act as integrators of different signaling pathways.
Fil: Trupkin, Santiago Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Auge, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Zhu, Jian-Kang. Purdue University; Estados Unidos. Chinese Academy of Sciences; China
Fil: Sanchez, Rodolfo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Materia
ARABIDOPSIS THALIANA
LIGHT SIGNALING
SALT SIGNALING
SALT OVERLY SENSITIVE (SOS) PATHWAY
CROSS TALK
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20929
Acceder
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network_name_str CONICET Digital (CONICET)
spelling SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in ArabidopsisTrupkin, Santiago ArielAuge, Gabriela AlejandraZhu, Jian-KangSanchez, Rodolfo AugustoBotto, Javier FranciscoARABIDOPSIS THALIANALIGHT SIGNALINGSALT SIGNALINGSALT OVERLY SENSITIVE (SOS) PATHWAYCROSS TALKhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Premise of research. Plants cope with complex environments throughout their life cycles. The perception and integration of information acquired from different environmental parameters are key to responding appropriately and securing plant survival. In Arabidopsis thaliana, SALT OVERLY SENSITIVE 2 (SOS2) and SOS3, its protein-interacting partner, have been described as central players in salt-stress responses, while another SOS2-interacting partner, ABSCISIC ACID (ABA)–INSENSITIVE 2 (ABI2), is involved in ABA signaling. SOS2 was also suggested to be involved in the photocontrol of seed germination, although its function in photomorphogenesis is not fully understood. Here we studied the role played by SOS2, SOS3, and ABI2 in light-dependent responses in Arabidopsis mutants. We assessed whether SOS2, SOS3, and ABI2 modulate light- and hormone-regulated seed germination and seedling deetiolation. Methodology. We examined physiological responses in wild-type and SOS2, SOS3, and ABI2 knockout mutants of A. thaliana exposed to red light and explored whether these proteins modulate germination (by changing hormone sensitivity) and seedling deetiolation. In addition, we analyzed the presence of cis-regulatory elements (CREs) in their promoters and expression profiles from public microarrays to confirm their function in photomorphogenesis. Pivotal results. Germination experiments demonstrate that SOS2, SOS3, and ABI2 are positive regulators of germination induced by red light. Differences in germination between the wild type and mutants are explained by changes in gibberellin sensitivity. Analysis of hypocotyl growth inhibition and cotyledon opening suggest that these components are also required for full seedling deetiolation under red light. Furthermore, identification of CREs in gene promoters and expression analysis matched the proposed role for these genes in both processes. Conclusions. SOS2, SOS3, and ABI2 are known components of salt signaling, and here we demonstrate that they are also positive elements of light signaling by regulating seedling deetiolation and altering gibberellic acid sensitivity during germination. These results suggest that they act as integrators of different signaling pathways.Fil: Trupkin, Santiago Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Auge, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Zhu, Jian-Kang. Purdue University; Estados Unidos. Chinese Academy of Sciences; ChinaFil: Sanchez, Rodolfo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaUniversity of Chicago Press2017-05info:eu-repo/date/embargoEnd/2018-05-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20929Trupkin, Santiago Ariel; Auge, Gabriela Alejandra; Zhu, Jian-Kang; Sanchez, Rodolfo Augusto; Botto, Javier Francisco; SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis; University of Chicago Press; International Journal of Plant Sciences; 178; 6; 5-2017; 485-4931058-58931058-5893CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.journals.uchicago.edu/doi/10.1086/692097info:eu-repo/semantics/altIdentifier/doi/10.1086/692097info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:07Zoai:ri.conicet.gov.ar:11336/20929instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:07.891CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
title SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
spellingShingle SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
Trupkin, Santiago Ariel
ARABIDOPSIS THALIANA
LIGHT SIGNALING
SALT SIGNALING
SALT OVERLY SENSITIVE (SOS) PATHWAY
CROSS TALK
title_short SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
title_full SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
title_fullStr SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
title_full_unstemmed SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
title_sort SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis
dc.creator.none.fl_str_mv Trupkin, Santiago Ariel
Auge, Gabriela Alejandra
Zhu, Jian-Kang
Sanchez, Rodolfo Augusto
Botto, Javier Francisco
author Trupkin, Santiago Ariel
author_facet Trupkin, Santiago Ariel
Auge, Gabriela Alejandra
Zhu, Jian-Kang
Sanchez, Rodolfo Augusto
Botto, Javier Francisco
author_role author
author2 Auge, Gabriela Alejandra
Zhu, Jian-Kang
Sanchez, Rodolfo Augusto
Botto, Javier Francisco
author2_role author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS THALIANA
LIGHT SIGNALING
SALT SIGNALING
SALT OVERLY SENSITIVE (SOS) PATHWAY
CROSS TALK
topic ARABIDOPSIS THALIANA
LIGHT SIGNALING
SALT SIGNALING
SALT OVERLY SENSITIVE (SOS) PATHWAY
CROSS TALK
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Premise of research. Plants cope with complex environments throughout their life cycles. The perception and integration of information acquired from different environmental parameters are key to responding appropriately and securing plant survival. In Arabidopsis thaliana, SALT OVERLY SENSITIVE 2 (SOS2) and SOS3, its protein-interacting partner, have been described as central players in salt-stress responses, while another SOS2-interacting partner, ABSCISIC ACID (ABA)–INSENSITIVE 2 (ABI2), is involved in ABA signaling. SOS2 was also suggested to be involved in the photocontrol of seed germination, although its function in photomorphogenesis is not fully understood. Here we studied the role played by SOS2, SOS3, and ABI2 in light-dependent responses in Arabidopsis mutants. We assessed whether SOS2, SOS3, and ABI2 modulate light- and hormone-regulated seed germination and seedling deetiolation. Methodology. We examined physiological responses in wild-type and SOS2, SOS3, and ABI2 knockout mutants of A. thaliana exposed to red light and explored whether these proteins modulate germination (by changing hormone sensitivity) and seedling deetiolation. In addition, we analyzed the presence of cis-regulatory elements (CREs) in their promoters and expression profiles from public microarrays to confirm their function in photomorphogenesis. Pivotal results. Germination experiments demonstrate that SOS2, SOS3, and ABI2 are positive regulators of germination induced by red light. Differences in germination between the wild type and mutants are explained by changes in gibberellin sensitivity. Analysis of hypocotyl growth inhibition and cotyledon opening suggest that these components are also required for full seedling deetiolation under red light. Furthermore, identification of CREs in gene promoters and expression analysis matched the proposed role for these genes in both processes. Conclusions. SOS2, SOS3, and ABI2 are known components of salt signaling, and here we demonstrate that they are also positive elements of light signaling by regulating seedling deetiolation and altering gibberellic acid sensitivity during germination. These results suggest that they act as integrators of different signaling pathways.
Fil: Trupkin, Santiago Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Auge, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Zhu, Jian-Kang. Purdue University; Estados Unidos. Chinese Academy of Sciences; China
Fil: Sanchez, Rodolfo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
description Premise of research. Plants cope with complex environments throughout their life cycles. The perception and integration of information acquired from different environmental parameters are key to responding appropriately and securing plant survival. In Arabidopsis thaliana, SALT OVERLY SENSITIVE 2 (SOS2) and SOS3, its protein-interacting partner, have been described as central players in salt-stress responses, while another SOS2-interacting partner, ABSCISIC ACID (ABA)–INSENSITIVE 2 (ABI2), is involved in ABA signaling. SOS2 was also suggested to be involved in the photocontrol of seed germination, although its function in photomorphogenesis is not fully understood. Here we studied the role played by SOS2, SOS3, and ABI2 in light-dependent responses in Arabidopsis mutants. We assessed whether SOS2, SOS3, and ABI2 modulate light- and hormone-regulated seed germination and seedling deetiolation. Methodology. We examined physiological responses in wild-type and SOS2, SOS3, and ABI2 knockout mutants of A. thaliana exposed to red light and explored whether these proteins modulate germination (by changing hormone sensitivity) and seedling deetiolation. In addition, we analyzed the presence of cis-regulatory elements (CREs) in their promoters and expression profiles from public microarrays to confirm their function in photomorphogenesis. Pivotal results. Germination experiments demonstrate that SOS2, SOS3, and ABI2 are positive regulators of germination induced by red light. Differences in germination between the wild type and mutants are explained by changes in gibberellin sensitivity. Analysis of hypocotyl growth inhibition and cotyledon opening suggest that these components are also required for full seedling deetiolation under red light. Furthermore, identification of CREs in gene promoters and expression analysis matched the proposed role for these genes in both processes. Conclusions. SOS2, SOS3, and ABI2 are known components of salt signaling, and here we demonstrate that they are also positive elements of light signaling by regulating seedling deetiolation and altering gibberellic acid sensitivity during germination. These results suggest that they act as integrators of different signaling pathways.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
info:eu-repo/date/embargoEnd/2018-05-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20929
Trupkin, Santiago Ariel; Auge, Gabriela Alejandra; Zhu, Jian-Kang; Sanchez, Rodolfo Augusto; Botto, Javier Francisco; SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis; University of Chicago Press; International Journal of Plant Sciences; 178; 6; 5-2017; 485-493
1058-5893
1058-5893
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20929
identifier_str_mv Trupkin, Santiago Ariel; Auge, Gabriela Alejandra; Zhu, Jian-Kang; Sanchez, Rodolfo Augusto; Botto, Javier Francisco; SALT OVERLY SENSITIVE 2 (SOS2) and Interacting Partners SOS3 and ABSCISIC ACID–INSENSITIVE 2 (ABI2) Promote Red-Light-Dependent Germination and Seedling Deetiolation in Arabidopsis; University of Chicago Press; International Journal of Plant Sciences; 178; 6; 5-2017; 485-493
1058-5893
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1086/692097
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
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eu_rights_str_mv embargoedAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Chicago Press
publisher.none.fl_str_mv University of Chicago Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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