Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis

Autores
Perez, Oscar Edgardo; David Birman, Tatyana; Kesselman, Ellina; Levi Tal, Sharon; Lesmes, Uri
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Growing attempts are being made to rationally utilize foods for human health improvement and disease prevention. Milk proteins are well suited for this purpose, since they are widely consumed, offer nutritional benefits and have been shown to be potentially suitable carriers for bioactive ingredients, such as vitamins and nutraceuticals. This work characterizes the interactions between β-lactoglobulin (β-lg) and folic acid (FA) at different load ratio and their functional implications, in terms of colloidal behavior and digestibility. Dynamic light scattering, isothermal titration calorimetery and atomic force microscopy were used to investigate β-lg/FA nano-complexes (mean size < 10 nm) formed at protein:vitamin molar ratio 1:10, whereas three FA molecules were found to be bound to one protein molecule. Colloidal stability tests (3 < pH < 10) revealed that nano-complexes formation improved β-lg dissolution near its isoelectric point and at low pH-values. This was also found to be accompanied by a shift in zeta-potential values at pH = 5 for pure β-lg (0.95 ± 0.09 mV) versus β-lg/FA nano-complexes (−20.13 ± 1.29 mV). SDS-PAGE analysis of digesta, collected from gastric and duodenal in vitro digestion of β-lg and its nano-complexes, revealed no marked alterations in the proteolytic susceptibility of β-lg. The study findings show the interactions of FA and β-lg in the formation of nano-complexes may be harnessed for delivery of FA in clear beverages with minimal effects to the protein's sensitivity to proteolysis.
Fil: Perez, Oscar Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: David Birman, Tatyana. Technion - Israel Institute of Technology; Israel
Fil: Kesselman, Ellina. Technion - Israel Institute of Technology; Israel
Fil: Levi Tal, Sharon. Technion - Israel Institute of Technology; Israel
Fil: Lesmes, Uri. Technion - Israel Institute of Technology; Israel
Materia
Β-Lactoglobulin
Folic Acid
Nano-Complexes
In Vitro Digestion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30645
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30645
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysisPerez, Oscar EdgardoDavid Birman, TatyanaKesselman, EllinaLevi Tal, SharonLesmes, UriΒ-LactoglobulinFolic AcidNano-ComplexesIn Vitro Digestionhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Growing attempts are being made to rationally utilize foods for human health improvement and disease prevention. Milk proteins are well suited for this purpose, since they are widely consumed, offer nutritional benefits and have been shown to be potentially suitable carriers for bioactive ingredients, such as vitamins and nutraceuticals. This work characterizes the interactions between β-lactoglobulin (β-lg) and folic acid (FA) at different load ratio and their functional implications, in terms of colloidal behavior and digestibility. Dynamic light scattering, isothermal titration calorimetery and atomic force microscopy were used to investigate β-lg/FA nano-complexes (mean size < 10 nm) formed at protein:vitamin molar ratio 1:10, whereas three FA molecules were found to be bound to one protein molecule. Colloidal stability tests (3 < pH < 10) revealed that nano-complexes formation improved β-lg dissolution near its isoelectric point and at low pH-values. This was also found to be accompanied by a shift in zeta-potential values at pH = 5 for pure β-lg (0.95 ± 0.09 mV) versus β-lg/FA nano-complexes (−20.13 ± 1.29 mV). SDS-PAGE analysis of digesta, collected from gastric and duodenal in vitro digestion of β-lg and its nano-complexes, revealed no marked alterations in the proteolytic susceptibility of β-lg. The study findings show the interactions of FA and β-lg in the formation of nano-complexes may be harnessed for delivery of FA in clear beverages with minimal effects to the protein's sensitivity to proteolysis.Fil: Perez, Oscar Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: David Birman, Tatyana. Technion - Israel Institute of Technology; IsraelFil: Kesselman, Ellina. Technion - Israel Institute of Technology; IsraelFil: Levi Tal, Sharon. Technion - Israel Institute of Technology; IsraelFil: Lesmes, Uri. Technion - Israel Institute of Technology; IsraelElsevier2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30645Perez, Oscar Edgardo; David Birman, Tatyana ; Kesselman, Ellina; Levi Tal, Sharon; Lesmes, Uri; Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis; Elsevier; Food Hydrocolloids; 38; 11-2013; 40-470268-005XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2013.11.010info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X13003718info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:35Zoai:ri.conicet.gov.ar:11336/30645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:35.444CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
title Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
spellingShingle Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
Perez, Oscar Edgardo
Β-Lactoglobulin
Folic Acid
Nano-Complexes
In Vitro Digestion
title_short Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
title_full Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
title_fullStr Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
title_full_unstemmed Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
title_sort Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis
dc.creator.none.fl_str_mv Perez, Oscar Edgardo
David Birman, Tatyana
Kesselman, Ellina
Levi Tal, Sharon
Lesmes, Uri
author Perez, Oscar Edgardo
author_facet Perez, Oscar Edgardo
David Birman, Tatyana
Kesselman, Ellina
Levi Tal, Sharon
Lesmes, Uri
author_role author
author2 David Birman, Tatyana
Kesselman, Ellina
Levi Tal, Sharon
Lesmes, Uri
author2_role author
author
author
author
dc.subject.none.fl_str_mv Β-Lactoglobulin
Folic Acid
Nano-Complexes
In Vitro Digestion
topic Β-Lactoglobulin
Folic Acid
Nano-Complexes
In Vitro Digestion
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Growing attempts are being made to rationally utilize foods for human health improvement and disease prevention. Milk proteins are well suited for this purpose, since they are widely consumed, offer nutritional benefits and have been shown to be potentially suitable carriers for bioactive ingredients, such as vitamins and nutraceuticals. This work characterizes the interactions between β-lactoglobulin (β-lg) and folic acid (FA) at different load ratio and their functional implications, in terms of colloidal behavior and digestibility. Dynamic light scattering, isothermal titration calorimetery and atomic force microscopy were used to investigate β-lg/FA nano-complexes (mean size < 10 nm) formed at protein:vitamin molar ratio 1:10, whereas three FA molecules were found to be bound to one protein molecule. Colloidal stability tests (3 < pH < 10) revealed that nano-complexes formation improved β-lg dissolution near its isoelectric point and at low pH-values. This was also found to be accompanied by a shift in zeta-potential values at pH = 5 for pure β-lg (0.95 ± 0.09 mV) versus β-lg/FA nano-complexes (−20.13 ± 1.29 mV). SDS-PAGE analysis of digesta, collected from gastric and duodenal in vitro digestion of β-lg and its nano-complexes, revealed no marked alterations in the proteolytic susceptibility of β-lg. The study findings show the interactions of FA and β-lg in the formation of nano-complexes may be harnessed for delivery of FA in clear beverages with minimal effects to the protein's sensitivity to proteolysis.
Fil: Perez, Oscar Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: David Birman, Tatyana. Technion - Israel Institute of Technology; Israel
Fil: Kesselman, Ellina. Technion - Israel Institute of Technology; Israel
Fil: Levi Tal, Sharon. Technion - Israel Institute of Technology; Israel
Fil: Lesmes, Uri. Technion - Israel Institute of Technology; Israel
description Growing attempts are being made to rationally utilize foods for human health improvement and disease prevention. Milk proteins are well suited for this purpose, since they are widely consumed, offer nutritional benefits and have been shown to be potentially suitable carriers for bioactive ingredients, such as vitamins and nutraceuticals. This work characterizes the interactions between β-lactoglobulin (β-lg) and folic acid (FA) at different load ratio and their functional implications, in terms of colloidal behavior and digestibility. Dynamic light scattering, isothermal titration calorimetery and atomic force microscopy were used to investigate β-lg/FA nano-complexes (mean size < 10 nm) formed at protein:vitamin molar ratio 1:10, whereas three FA molecules were found to be bound to one protein molecule. Colloidal stability tests (3 < pH < 10) revealed that nano-complexes formation improved β-lg dissolution near its isoelectric point and at low pH-values. This was also found to be accompanied by a shift in zeta-potential values at pH = 5 for pure β-lg (0.95 ± 0.09 mV) versus β-lg/FA nano-complexes (−20.13 ± 1.29 mV). SDS-PAGE analysis of digesta, collected from gastric and duodenal in vitro digestion of β-lg and its nano-complexes, revealed no marked alterations in the proteolytic susceptibility of β-lg. The study findings show the interactions of FA and β-lg in the formation of nano-complexes may be harnessed for delivery of FA in clear beverages with minimal effects to the protein's sensitivity to proteolysis.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30645
Perez, Oscar Edgardo; David Birman, Tatyana ; Kesselman, Ellina; Levi Tal, Sharon; Lesmes, Uri; Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis; Elsevier; Food Hydrocolloids; 38; 11-2013; 40-47
0268-005X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30645
identifier_str_mv Perez, Oscar Edgardo; David Birman, Tatyana ; Kesselman, Ellina; Levi Tal, Sharon; Lesmes, Uri; Milk protein-vitamin interactions: Formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis; Elsevier; Food Hydrocolloids; 38; 11-2013; 40-47
0268-005X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2013.11.010
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X13003718
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083078156451840
score 13.22299

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