Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine

Autores
Araya, Roberto; Andino Pavlovsky, Victoria; Yuste, Rafael; Etchenique, Roberto Argentino
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.
Fil: Araya, Roberto. Columbia University; Estados Unidos
Fil: Andino Pavlovsky, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Yuste, Rafael. Columbia University; Estados Unidos
Fil: Etchenique, Roberto Argentino. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Materia
Dopamine
Ruthenium
Two Photon
Caged Compound
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8018
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8018
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged DopamineAraya, RobertoAndino Pavlovsky, VictoriaYuste, RafaelEtchenique, Roberto ArgentinoDopamineRutheniumTwo PhotonCaged Compoundhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.Fil: Araya, Roberto. Columbia University; Estados UnidosFil: Andino Pavlovsky, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaFil: Yuste, Rafael. Columbia University; Estados UnidosFil: Etchenique, Roberto Argentino. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaAmerican Chemical Society2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8018Araya, Roberto; Andino Pavlovsky, Victoria; Yuste, Rafael; Etchenique, Roberto Argentino; Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine; American Chemical Society; ACS Chemical Neuroscience; 4; 8; 5-2013; 1163-11671948-7193enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1021/cn4000692info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/cn4000692info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:18:33Zoai:ri.conicet.gov.ar:11336/8018instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:18:34.167CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
title Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
spellingShingle Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
Araya, Roberto
Dopamine
Ruthenium
Two Photon
Caged Compound
title_short Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
title_full Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
title_fullStr Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
title_full_unstemmed Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
title_sort Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
dc.creator.none.fl_str_mv Araya, Roberto
Andino Pavlovsky, Victoria
Yuste, Rafael
Etchenique, Roberto Argentino
author Araya, Roberto
author_facet Araya, Roberto
Andino Pavlovsky, Victoria
Yuste, Rafael
Etchenique, Roberto Argentino
author_role author
author2 Andino Pavlovsky, Victoria
Yuste, Rafael
Etchenique, Roberto Argentino
author2_role author
author
author
dc.subject.none.fl_str_mv Dopamine
Ruthenium
Two Photon
Caged Compound
topic Dopamine
Ruthenium
Two Photon
Caged Compound
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.
Fil: Araya, Roberto. Columbia University; Estados Unidos
Fil: Andino Pavlovsky, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Yuste, Rafael. Columbia University; Estados Unidos
Fil: Etchenique, Roberto Argentino. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de Los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
description We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8018
Araya, Roberto; Andino Pavlovsky, Victoria; Yuste, Rafael; Etchenique, Roberto Argentino; Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine; American Chemical Society; ACS Chemical Neuroscience; 4; 8; 5-2013; 1163-1167
1948-7193
url http://hdl.handle.net/11336/8018
identifier_str_mv Araya, Roberto; Andino Pavlovsky, Victoria; Yuste, Rafael; Etchenique, Roberto Argentino; Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine; American Chemical Society; ACS Chemical Neuroscience; 4; 8; 5-2013; 1163-1167
1948-7193
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1021/cn4000692
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/cn4000692
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.124843

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