Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokine...

Autores
Plá, Natalia; Chop, Maia; Nicolao, María Celeste; Cumino, Andrea Carina; Rodríguez Rodrígues, Christian Fernando Ariel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: The laminar layer is an acellular structure rich in glycans that surrounds the metacestode, creating a mechanical and immunological protective barrier, crucial in the Echinococcus?host interface. The aim of this work is to analyze if purified laminar layer (pLL) could trigger maturation and cytokine production in dendritic cells by activating mTOR pathway, a central regulator of cell metabolism and environmental signals. Methods: BMDCs were cultured in complete RPMI supplemented with 100 ng/ml Flt3-L.The germinal layer was removed from pLL by washing with 2M NaCl. Flow cytometry: FITC or PE-conjugated mAbs directed to CD11c, CD40, CD80, CD86, MHC I and MHC II were used. Immunobloting and confocal microscopy: mouse anti-puromycin and rabbit anti-Phospho-mTOR antibodies were used. Gene expression analysis of IL-10, IL-12p35, IL-6, TNF-, IL-23p19, TGF- was performed on a Real Time PCR System Results: The pLL of Echinococcus induce a non-statistically significant, but a trend in the up-regulation of CD86 and MHC II and that expression change was diminished by the use of rapamycin (n=5). Conversely, no changes in the expression of CD40, CD80 or MHC I were registered. Further studies were done to analyze whether pLL was also able to stimulate the production of cytokines by BMDCs. When BMDCs were stimulated with pLL induce the expression of IL-6 and TNF-an=3 *p<0.05, **p<0.01), but not differences compared to control were observed in IL-12, IL-23, IL-10 and TGF-. Finally, we evaluated global protein synthesis and phosphorylation of mTOR using confocal microscopy or western blot. pLL (MFI:_979) showed an increase in translation levels compared to its counterpart without stimulation (MFI:_686, *p<0.05 n=3) and also induce an increase in mTOR phosphorylation levels compared to untreated BMCDs (n=3, p<0.01) Conclusions: These data suggest that Echinococcus pLL are recognized by DCs and induce activation of mTOR pathway favoring cell activation.
Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
Mucins
laminar layer
Echinococcus granulosus
dendritic cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154552
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154552
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokinesPlá, NataliaChop, MaiaNicolao, María CelesteCumino, Andrea CarinaRodríguez Rodrígues, Christian Fernando ArielMucinslaminar layerEchinococcus granulosusdendritic cellshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: The laminar layer is an acellular structure rich in glycans that surrounds the metacestode, creating a mechanical and immunological protective barrier, crucial in the Echinococcus?host interface. The aim of this work is to analyze if purified laminar layer (pLL) could trigger maturation and cytokine production in dendritic cells by activating mTOR pathway, a central regulator of cell metabolism and environmental signals. Methods: BMDCs were cultured in complete RPMI supplemented with 100 ng/ml Flt3-L.The germinal layer was removed from pLL by washing with 2M NaCl. Flow cytometry: FITC or PE-conjugated mAbs directed to CD11c, CD40, CD80, CD86, MHC I and MHC II were used. Immunobloting and confocal microscopy: mouse anti-puromycin and rabbit anti-Phospho-mTOR antibodies were used. Gene expression analysis of IL-10, IL-12p35, IL-6, TNF-, IL-23p19, TGF- was performed on a Real Time PCR System Results: The pLL of Echinococcus induce a non-statistically significant, but a trend in the up-regulation of CD86 and MHC II and that expression change was diminished by the use of rapamycin (n=5). Conversely, no changes in the expression of CD40, CD80 or MHC I were registered. Further studies were done to analyze whether pLL was also able to stimulate the production of cytokines by BMDCs. When BMDCs were stimulated with pLL induce the expression of IL-6 and TNF-an=3 *p<0.05, **p<0.01), but not differences compared to control were observed in IL-12, IL-23, IL-10 and TGF-. Finally, we evaluated global protein synthesis and phosphorylation of mTOR using confocal microscopy or western blot. pLL (MFI:_979) showed an increase in translation levels compared to its counterpart without stimulation (MFI:_686, *p<0.05 n=3) and also induce an increase in mTOR phosphorylation levels compared to untreated BMCDs (n=3, p<0.01) Conclusions: These data suggest that Echinococcus pLL are recognized by DCs and induce activation of mTOR pathway favoring cell activation.Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaLXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154552Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 153-1540025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol80-20/s5/Mv80s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:57Zoai:ri.conicet.gov.ar:11336/154552instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:57.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
title Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
spellingShingle Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
Plá, Natalia
Mucins
laminar layer
Echinococcus granulosus
dendritic cells
title_short Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
title_full Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
title_fullStr Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
title_full_unstemmed Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
title_sort Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
dc.creator.none.fl_str_mv Plá, Natalia
Chop, Maia
Nicolao, María Celeste
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author Plá, Natalia
author_facet Plá, Natalia
Chop, Maia
Nicolao, María Celeste
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author_role author
author2 Chop, Maia
Nicolao, María Celeste
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mucins
laminar layer
Echinococcus granulosus
dendritic cells
topic Mucins
laminar layer
Echinococcus granulosus
dendritic cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: The laminar layer is an acellular structure rich in glycans that surrounds the metacestode, creating a mechanical and immunological protective barrier, crucial in the Echinococcus?host interface. The aim of this work is to analyze if purified laminar layer (pLL) could trigger maturation and cytokine production in dendritic cells by activating mTOR pathway, a central regulator of cell metabolism and environmental signals. Methods: BMDCs were cultured in complete RPMI supplemented with 100 ng/ml Flt3-L.The germinal layer was removed from pLL by washing with 2M NaCl. Flow cytometry: FITC or PE-conjugated mAbs directed to CD11c, CD40, CD80, CD86, MHC I and MHC II were used. Immunobloting and confocal microscopy: mouse anti-puromycin and rabbit anti-Phospho-mTOR antibodies were used. Gene expression analysis of IL-10, IL-12p35, IL-6, TNF-, IL-23p19, TGF- was performed on a Real Time PCR System Results: The pLL of Echinococcus induce a non-statistically significant, but a trend in the up-regulation of CD86 and MHC II and that expression change was diminished by the use of rapamycin (n=5). Conversely, no changes in the expression of CD40, CD80 or MHC I were registered. Further studies were done to analyze whether pLL was also able to stimulate the production of cytokines by BMDCs. When BMDCs were stimulated with pLL induce the expression of IL-6 and TNF-an=3 *p<0.05, **p<0.01), but not differences compared to control were observed in IL-12, IL-23, IL-10 and TGF-. Finally, we evaluated global protein synthesis and phosphorylation of mTOR using confocal microscopy or western blot. pLL (MFI:_979) showed an increase in translation levels compared to its counterpart without stimulation (MFI:_686, *p<0.05 n=3) and also induce an increase in mTOR phosphorylation levels compared to untreated BMCDs (n=3, p<0.01) Conclusions: These data suggest that Echinococcus pLL are recognized by DCs and induce activation of mTOR pathway favoring cell activation.
Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Background: The laminar layer is an acellular structure rich in glycans that surrounds the metacestode, creating a mechanical and immunological protective barrier, crucial in the Echinococcus?host interface. The aim of this work is to analyze if purified laminar layer (pLL) could trigger maturation and cytokine production in dendritic cells by activating mTOR pathway, a central regulator of cell metabolism and environmental signals. Methods: BMDCs were cultured in complete RPMI supplemented with 100 ng/ml Flt3-L.The germinal layer was removed from pLL by washing with 2M NaCl. Flow cytometry: FITC or PE-conjugated mAbs directed to CD11c, CD40, CD80, CD86, MHC I and MHC II were used. Immunobloting and confocal microscopy: mouse anti-puromycin and rabbit anti-Phospho-mTOR antibodies were used. Gene expression analysis of IL-10, IL-12p35, IL-6, TNF-, IL-23p19, TGF- was performed on a Real Time PCR System Results: The pLL of Echinococcus induce a non-statistically significant, but a trend in the up-regulation of CD86 and MHC II and that expression change was diminished by the use of rapamycin (n=5). Conversely, no changes in the expression of CD40, CD80 or MHC I were registered. Further studies were done to analyze whether pLL was also able to stimulate the production of cytokines by BMDCs. When BMDCs were stimulated with pLL induce the expression of IL-6 and TNF-an=3 *p<0.05, **p<0.01), but not differences compared to control were observed in IL-12, IL-23, IL-10 and TGF-. Finally, we evaluated global protein synthesis and phosphorylation of mTOR using confocal microscopy or western blot. pLL (MFI:_979) showed an increase in translation levels compared to its counterpart without stimulation (MFI:_686, *p<0.05 n=3) and also induce an increase in mTOR phosphorylation levels compared to untreated BMCDs (n=3, p<0.01) Conclusions: These data suggest that Echinococcus pLL are recognized by DCs and induce activation of mTOR pathway favoring cell activation.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154552
Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 153-154
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154552
identifier_str_mv Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 153-154
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol80-20/s5/Mv80s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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