Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
- Autores
- Reinoso, Elina Beatríz; Bettera, Susana Gertrudis
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak.
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bettera, Susana Gertrudis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina - Materia
-
LABORATORY EXERCISES
MOLECULAR EPIDEMIOLOGY
RAPD-PCR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/71749
Ver los metadatos del registro completo
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Random amplified polymorphic DNA PCR in the teaching of molecular epidemiologyReinoso, Elina BeatrízBettera, Susana GertrudisLABORATORY EXERCISESMOLECULAR EPIDEMIOLOGYRAPD-PCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak.Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bettera, Susana Gertrudis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaJohn Wiley & Sons Inc2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/71749Reinoso, Elina Beatríz; Bettera, Susana Gertrudis; Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 44; 4; 7-2016; 391-3961470-81751539-3429CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.20957info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20957info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:04Zoai:ri.conicet.gov.ar:11336/71749instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:04.405CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology |
title |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology |
spellingShingle |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology Reinoso, Elina Beatríz LABORATORY EXERCISES MOLECULAR EPIDEMIOLOGY RAPD-PCR |
title_short |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology |
title_full |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology |
title_fullStr |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology |
title_full_unstemmed |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology |
title_sort |
Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology |
dc.creator.none.fl_str_mv |
Reinoso, Elina Beatríz Bettera, Susana Gertrudis |
author |
Reinoso, Elina Beatríz |
author_facet |
Reinoso, Elina Beatríz Bettera, Susana Gertrudis |
author_role |
author |
author2 |
Bettera, Susana Gertrudis |
author2_role |
author |
dc.subject.none.fl_str_mv |
LABORATORY EXERCISES MOLECULAR EPIDEMIOLOGY RAPD-PCR |
topic |
LABORATORY EXERCISES MOLECULAR EPIDEMIOLOGY RAPD-PCR |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak. Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bettera, Susana Gertrudis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina |
description |
In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/71749 Reinoso, Elina Beatríz; Bettera, Susana Gertrudis; Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 44; 4; 7-2016; 391-396 1470-8175 1539-3429 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/71749 |
identifier_str_mv |
Reinoso, Elina Beatríz; Bettera, Susana Gertrudis; Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 44; 4; 7-2016; 391-396 1470-8175 1539-3429 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.20957 info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20957 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley & Sons Inc |
publisher.none.fl_str_mv |
John Wiley & Sons Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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