Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone

Autores
Sutton Mcdowall, Melanie L.; Purdey, Malcom; Brown, Hannah; Abell, Andrew D.; Mottershead, David G.; Cetica, Pablo Daniel; Dalvit, Gabriel C.; Goldys, Ewa M.; Gilchrist, Robert B.; Gardner, David K.; Thompson, Jeremy G.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The developmental competence of cumulus oocyte complexes (COCs) can be increased during in vitro oocyte maturation with the addition of exogenous oocyte-secreted factors, such as bone morphogenetic protein 15 (BMP15), in combination with hormones. FSH and BMP15, for example, induce different metabolic profiles within COCs—namely, FSH increases glycolysis while BMP15 stimulates FAD and NAD(P)H accumulation within oocytes, without changing the redox ratio. The aim of this study was to investigate if this BMP15-induced NAD(P)H increase was due to de novo NADPH production. Cattle COCs were cultured with FSH and/or recombinant human BMP15, resulting in a significant decrease in glucose-6-phosphate dehydrogenase activity (P < 0.05). Inhibition of isocitrate dehydrogenase (IDH) during this process decreased NAD(P)H intensity threefold in BMP15-treated oocytes, suggesting that BMP15 stimulates IDH and NADPH production via the tricarboxylic acid cycle. As NADPH is a reducing agent, reduced glutathione (GSH), H2O2, and mitochondrial activity were also measured to assess the general redox status of the oocyte. FSH alone decreased GSH levels whereas the combination of BMP15 and FSH sustained higher levels. Expression of genes encoding glutathione-reducing enzymes were also lower in oocytes cultured in the presence of FSH alone. BMP15 supplementation further promoted mitochondrial localization patterns that are consistent with enhanced developmental competence. Metabolomics revealed significant consumption of glutamine and production of alanine by COCs matured with both FSH and BMP15 compared to the control (P < 0.05). Hence, BMP15 supplementation differentially modulates reductive metabolism and mitochondrial localization within the oocyte. In comparison, FSH-stimulation alone decreases the oocytes’ ability to regulate cellular stress, and therefore utilizes other mechanisms to improve developmental competence.
Fil: Sutton Mcdowall, Melanie L.. University Of Adelaide; Australia. Australian Research Council; Australia
Fil: Purdey, Malcom. Australian Research Council; Australia
Fil: Brown, Hannah. University Of Adelaide; Australia
Fil: Abell, Andrew D.. Australian Research Council; Australia
Fil: Mottershead, David G.. University Of Adelaide; Australia
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Produccion Animal; Argentina
Fil: Dalvit, Gabriel C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Produccion Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Estudios Transdisciplinarios del Agua; Argentina
Fil: Goldys, Ewa M.. Australian Research Council; Australia. Macquarie University; Australia
Fil: Gilchrist, Robert B.. University Of Adelaide; Australia. University Of New South Wales; Australia
Fil: Gardner, David K.. The University Of Melbourne; Australia
Fil: Thompson, Jeremy G.. University Of Adelaide; Australia. Australian Research Council; Australia
Materia
Redox State
Anti-Oxidant State
Bmp15
Fsh
In Vitro Maturation
Oocytes
Bovine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8095

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oai_identifier_str oai:ri.conicet.gov.ar:11336/8095
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormoneSutton Mcdowall, Melanie L.Purdey, MalcomBrown, HannahAbell, Andrew D.Mottershead, David G.Cetica, Pablo DanielDalvit, Gabriel C.Goldys, Ewa M.Gilchrist, Robert B.Gardner, David K.Thompson, Jeremy G.Redox StateAnti-Oxidant StateBmp15FshIn Vitro MaturationOocytesBovinehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The developmental competence of cumulus oocyte complexes (COCs) can be increased during in vitro oocyte maturation with the addition of exogenous oocyte-secreted factors, such as bone morphogenetic protein 15 (BMP15), in combination with hormones. FSH and BMP15, for example, induce different metabolic profiles within COCs—namely, FSH increases glycolysis while BMP15 stimulates FAD and NAD(P)H accumulation within oocytes, without changing the redox ratio. The aim of this study was to investigate if this BMP15-induced NAD(P)H increase was due to de novo NADPH production. Cattle COCs were cultured with FSH and/or recombinant human BMP15, resulting in a significant decrease in glucose-6-phosphate dehydrogenase activity (P < 0.05). Inhibition of isocitrate dehydrogenase (IDH) during this process decreased NAD(P)H intensity threefold in BMP15-treated oocytes, suggesting that BMP15 stimulates IDH and NADPH production via the tricarboxylic acid cycle. As NADPH is a reducing agent, reduced glutathione (GSH), H2O2, and mitochondrial activity were also measured to assess the general redox status of the oocyte. FSH alone decreased GSH levels whereas the combination of BMP15 and FSH sustained higher levels. Expression of genes encoding glutathione-reducing enzymes were also lower in oocytes cultured in the presence of FSH alone. BMP15 supplementation further promoted mitochondrial localization patterns that are consistent with enhanced developmental competence. Metabolomics revealed significant consumption of glutamine and production of alanine by COCs matured with both FSH and BMP15 compared to the control (P < 0.05). Hence, BMP15 supplementation differentially modulates reductive metabolism and mitochondrial localization within the oocyte. In comparison, FSH-stimulation alone decreases the oocytes’ ability to regulate cellular stress, and therefore utilizes other mechanisms to improve developmental competence.Fil: Sutton Mcdowall, Melanie L.. University Of Adelaide; Australia. Australian Research Council; AustraliaFil: Purdey, Malcom. Australian Research Council; AustraliaFil: Brown, Hannah. University Of Adelaide; AustraliaFil: Abell, Andrew D.. Australian Research Council; AustraliaFil: Mottershead, David G.. University Of Adelaide; AustraliaFil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Produccion Animal; ArgentinaFil: Dalvit, Gabriel C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Produccion Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Estudios Transdisciplinarios del Agua; ArgentinaFil: Goldys, Ewa M.. Australian Research Council; Australia. Macquarie University; AustraliaFil: Gilchrist, Robert B.. University Of Adelaide; Australia. University Of New South Wales; AustraliaFil: Gardner, David K.. The University Of Melbourne; AustraliaFil: Thompson, Jeremy G.. University Of Adelaide; Australia. Australian Research Council; AustraliaWiley-liss, Div John Wiley & Sons Inc2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8095Sutton Mcdowall, Melanie L.; Purdey, Malcom; Brown, Hannah; Abell, Andrew D.; Mottershead, David G.; et al.; Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction And Development; 82; 4; 4-2015; 281-2941040-452Xenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mrd.22470/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.22470info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:01Zoai:ri.conicet.gov.ar:11336/8095instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:01.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
title Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
spellingShingle Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
Sutton Mcdowall, Melanie L.
Redox State
Anti-Oxidant State
Bmp15
Fsh
In Vitro Maturation
Oocytes
Bovine
title_short Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
title_full Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
title_fullStr Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
title_full_unstemmed Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
title_sort Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone
dc.creator.none.fl_str_mv Sutton Mcdowall, Melanie L.
Purdey, Malcom
Brown, Hannah
Abell, Andrew D.
Mottershead, David G.
Cetica, Pablo Daniel
Dalvit, Gabriel C.
Goldys, Ewa M.
Gilchrist, Robert B.
Gardner, David K.
Thompson, Jeremy G.
author Sutton Mcdowall, Melanie L.
author_facet Sutton Mcdowall, Melanie L.
Purdey, Malcom
Brown, Hannah
Abell, Andrew D.
Mottershead, David G.
Cetica, Pablo Daniel
Dalvit, Gabriel C.
Goldys, Ewa M.
Gilchrist, Robert B.
Gardner, David K.
Thompson, Jeremy G.
author_role author
author2 Purdey, Malcom
Brown, Hannah
Abell, Andrew D.
Mottershead, David G.
Cetica, Pablo Daniel
Dalvit, Gabriel C.
Goldys, Ewa M.
Gilchrist, Robert B.
Gardner, David K.
Thompson, Jeremy G.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Redox State
Anti-Oxidant State
Bmp15
Fsh
In Vitro Maturation
Oocytes
Bovine
topic Redox State
Anti-Oxidant State
Bmp15
Fsh
In Vitro Maturation
Oocytes
Bovine
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The developmental competence of cumulus oocyte complexes (COCs) can be increased during in vitro oocyte maturation with the addition of exogenous oocyte-secreted factors, such as bone morphogenetic protein 15 (BMP15), in combination with hormones. FSH and BMP15, for example, induce different metabolic profiles within COCs—namely, FSH increases glycolysis while BMP15 stimulates FAD and NAD(P)H accumulation within oocytes, without changing the redox ratio. The aim of this study was to investigate if this BMP15-induced NAD(P)H increase was due to de novo NADPH production. Cattle COCs were cultured with FSH and/or recombinant human BMP15, resulting in a significant decrease in glucose-6-phosphate dehydrogenase activity (P < 0.05). Inhibition of isocitrate dehydrogenase (IDH) during this process decreased NAD(P)H intensity threefold in BMP15-treated oocytes, suggesting that BMP15 stimulates IDH and NADPH production via the tricarboxylic acid cycle. As NADPH is a reducing agent, reduced glutathione (GSH), H2O2, and mitochondrial activity were also measured to assess the general redox status of the oocyte. FSH alone decreased GSH levels whereas the combination of BMP15 and FSH sustained higher levels. Expression of genes encoding glutathione-reducing enzymes were also lower in oocytes cultured in the presence of FSH alone. BMP15 supplementation further promoted mitochondrial localization patterns that are consistent with enhanced developmental competence. Metabolomics revealed significant consumption of glutamine and production of alanine by COCs matured with both FSH and BMP15 compared to the control (P < 0.05). Hence, BMP15 supplementation differentially modulates reductive metabolism and mitochondrial localization within the oocyte. In comparison, FSH-stimulation alone decreases the oocytes’ ability to regulate cellular stress, and therefore utilizes other mechanisms to improve developmental competence.
Fil: Sutton Mcdowall, Melanie L.. University Of Adelaide; Australia. Australian Research Council; Australia
Fil: Purdey, Malcom. Australian Research Council; Australia
Fil: Brown, Hannah. University Of Adelaide; Australia
Fil: Abell, Andrew D.. Australian Research Council; Australia
Fil: Mottershead, David G.. University Of Adelaide; Australia
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Produccion Animal; Argentina
Fil: Dalvit, Gabriel C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Produccion Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Estudios Transdisciplinarios del Agua; Argentina
Fil: Goldys, Ewa M.. Australian Research Council; Australia. Macquarie University; Australia
Fil: Gilchrist, Robert B.. University Of Adelaide; Australia. University Of New South Wales; Australia
Fil: Gardner, David K.. The University Of Melbourne; Australia
Fil: Thompson, Jeremy G.. University Of Adelaide; Australia. Australian Research Council; Australia
description The developmental competence of cumulus oocyte complexes (COCs) can be increased during in vitro oocyte maturation with the addition of exogenous oocyte-secreted factors, such as bone morphogenetic protein 15 (BMP15), in combination with hormones. FSH and BMP15, for example, induce different metabolic profiles within COCs—namely, FSH increases glycolysis while BMP15 stimulates FAD and NAD(P)H accumulation within oocytes, without changing the redox ratio. The aim of this study was to investigate if this BMP15-induced NAD(P)H increase was due to de novo NADPH production. Cattle COCs were cultured with FSH and/or recombinant human BMP15, resulting in a significant decrease in glucose-6-phosphate dehydrogenase activity (P < 0.05). Inhibition of isocitrate dehydrogenase (IDH) during this process decreased NAD(P)H intensity threefold in BMP15-treated oocytes, suggesting that BMP15 stimulates IDH and NADPH production via the tricarboxylic acid cycle. As NADPH is a reducing agent, reduced glutathione (GSH), H2O2, and mitochondrial activity were also measured to assess the general redox status of the oocyte. FSH alone decreased GSH levels whereas the combination of BMP15 and FSH sustained higher levels. Expression of genes encoding glutathione-reducing enzymes were also lower in oocytes cultured in the presence of FSH alone. BMP15 supplementation further promoted mitochondrial localization patterns that are consistent with enhanced developmental competence. Metabolomics revealed significant consumption of glutamine and production of alanine by COCs matured with both FSH and BMP15 compared to the control (P < 0.05). Hence, BMP15 supplementation differentially modulates reductive metabolism and mitochondrial localization within the oocyte. In comparison, FSH-stimulation alone decreases the oocytes’ ability to regulate cellular stress, and therefore utilizes other mechanisms to improve developmental competence.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8095
Sutton Mcdowall, Melanie L.; Purdey, Malcom; Brown, Hannah; Abell, Andrew D.; Mottershead, David G.; et al.; Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction And Development; 82; 4; 4-2015; 281-294
1040-452X
url http://hdl.handle.net/11336/8095
identifier_str_mv Sutton Mcdowall, Melanie L.; Purdey, Malcom; Brown, Hannah; Abell, Andrew D.; Mottershead, David G.; et al.; Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenic protein 15 and follicle stimulating hormone; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction And Development; 82; 4; 4-2015; 281-294
1040-452X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mrd.22470/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.22470
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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