In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)

Autores
Abud, Julián Elías; Pagotto, Romina María del Luján; Galliani, Valentina; Teglia, Carla Mariela; Culzoni, Maria Julia; Bollati Fogolín, M.; Zenclussen, Maria Laura; Rodriguez, Horacio Adolfo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Benzophenone-3 (BP3), a common ingredient in personal care products (PCPs) due to its well-established effectiveness in blocking UV radiation, is considered an endocrine-disrupting chemical (EDC) mainly based on in vitro estrogenic and antiandrogenic effects. Sunscreen products are among the most widelyused PCPs-containing BP3 applied to the skin, resulting in signicant human exposure to BP3 primarily through a dermal application. In the present work, we have tested the action of BP3 using an in vitro model of anchoring and implantation of murine blastocysts as well as by analyzing the migration abilityof the human trophoblast cell line Swan 71. BP3 concentrations used in our experiments were: a) 2 μg/L, based on the predicted no-effect concentration for aquatic environments; b) 20 μg/L, similar to the levels of BP3 detected in serum and amniotic uid of pregnant mice exposed to BP3 from gestation day 0 to gd6 by dermal exposure; and c) BP3-200 μg/L, based on plasma concentrations reached by BP3 after a whole body topical application of sunscreens in humans. We found that BP3 negatively affected critical parameters of both in vitro implantation and early embryo development and reduced trophoblast migration through the androgen receptor (AR) pathway. Based on these ndings, it can be concluded that BP3 can affect early in vitro embryo implantation and trophoblast migration capacity.
Fil: Abud, Julián Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Pagotto, Romina María del Luján. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Teglia, Carla Mariela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bollati Fogolín, M.. Instituto Pasteur de Montevideo; Uruguay
Fil: Zenclussen, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Materia
BP3
BENZOPHENONE-3
IMPLANTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/253476
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)Abud, Julián ElíasPagotto, Romina María del LujánGalliani, ValentinaTeglia, Carla MarielaCulzoni, Maria JuliaBollati Fogolín, M.Zenclussen, Maria LauraRodriguez, Horacio AdolfoBP3BENZOPHENONE-3IMPLANTATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Benzophenone-3 (BP3), a common ingredient in personal care products (PCPs) due to its well-established effectiveness in blocking UV radiation, is considered an endocrine-disrupting chemical (EDC) mainly based on in vitro estrogenic and antiandrogenic effects. Sunscreen products are among the most widelyused PCPs-containing BP3 applied to the skin, resulting in signicant human exposure to BP3 primarily through a dermal application. In the present work, we have tested the action of BP3 using an in vitro model of anchoring and implantation of murine blastocysts as well as by analyzing the migration abilityof the human trophoblast cell line Swan 71. BP3 concentrations used in our experiments were: a) 2 μg/L, based on the predicted no-effect concentration for aquatic environments; b) 20 μg/L, similar to the levels of BP3 detected in serum and amniotic uid of pregnant mice exposed to BP3 from gestation day 0 to gd6 by dermal exposure; and c) BP3-200 μg/L, based on plasma concentrations reached by BP3 after a whole body topical application of sunscreens in humans. We found that BP3 negatively affected critical parameters of both in vitro implantation and early embryo development and reduced trophoblast migration through the androgen receptor (AR) pathway. Based on these ndings, it can be concluded that BP3 can affect early in vitro embryo implantation and trophoblast migration capacity.Fil: Abud, Julián Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Pagotto, Romina María del Luján. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Teglia, Carla Mariela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; ArgentinaFil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bollati Fogolín, M.. Instituto Pasteur de Montevideo; UruguayFil: Zenclussen, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaSpringer2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/253476Abud, Julián Elías; Pagotto, Romina María del Luján; Galliani, Valentina; Teglia, Carla Mariela; Culzoni, Maria Julia; et al.; In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3); Springer; Research Square; 8-2023; 1-242693-5015CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-3210668/v1info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-3210668/v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:59Zoai:ri.conicet.gov.ar:11336/253476instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:59.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
title In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
spellingShingle In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
Abud, Julián Elías
BP3
BENZOPHENONE-3
IMPLANTATION
title_short In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
title_full In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
title_fullStr In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
title_full_unstemmed In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
title_sort In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3)
dc.creator.none.fl_str_mv Abud, Julián Elías
Pagotto, Romina María del Luján
Galliani, Valentina
Teglia, Carla Mariela
Culzoni, Maria Julia
Bollati Fogolín, M.
Zenclussen, Maria Laura
Rodriguez, Horacio Adolfo
author Abud, Julián Elías
author_facet Abud, Julián Elías
Pagotto, Romina María del Luján
Galliani, Valentina
Teglia, Carla Mariela
Culzoni, Maria Julia
Bollati Fogolín, M.
Zenclussen, Maria Laura
Rodriguez, Horacio Adolfo
author_role author
author2 Pagotto, Romina María del Luján
Galliani, Valentina
Teglia, Carla Mariela
Culzoni, Maria Julia
Bollati Fogolín, M.
Zenclussen, Maria Laura
Rodriguez, Horacio Adolfo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BP3
BENZOPHENONE-3
IMPLANTATION
topic BP3
BENZOPHENONE-3
IMPLANTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Benzophenone-3 (BP3), a common ingredient in personal care products (PCPs) due to its well-established effectiveness in blocking UV radiation, is considered an endocrine-disrupting chemical (EDC) mainly based on in vitro estrogenic and antiandrogenic effects. Sunscreen products are among the most widelyused PCPs-containing BP3 applied to the skin, resulting in signicant human exposure to BP3 primarily through a dermal application. In the present work, we have tested the action of BP3 using an in vitro model of anchoring and implantation of murine blastocysts as well as by analyzing the migration abilityof the human trophoblast cell line Swan 71. BP3 concentrations used in our experiments were: a) 2 μg/L, based on the predicted no-effect concentration for aquatic environments; b) 20 μg/L, similar to the levels of BP3 detected in serum and amniotic uid of pregnant mice exposed to BP3 from gestation day 0 to gd6 by dermal exposure; and c) BP3-200 μg/L, based on plasma concentrations reached by BP3 after a whole body topical application of sunscreens in humans. We found that BP3 negatively affected critical parameters of both in vitro implantation and early embryo development and reduced trophoblast migration through the androgen receptor (AR) pathway. Based on these ndings, it can be concluded that BP3 can affect early in vitro embryo implantation and trophoblast migration capacity.
Fil: Abud, Julián Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Pagotto, Romina María del Luján. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Teglia, Carla Mariela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bollati Fogolín, M.. Instituto Pasteur de Montevideo; Uruguay
Fil: Zenclussen, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
description Benzophenone-3 (BP3), a common ingredient in personal care products (PCPs) due to its well-established effectiveness in blocking UV radiation, is considered an endocrine-disrupting chemical (EDC) mainly based on in vitro estrogenic and antiandrogenic effects. Sunscreen products are among the most widelyused PCPs-containing BP3 applied to the skin, resulting in signicant human exposure to BP3 primarily through a dermal application. In the present work, we have tested the action of BP3 using an in vitro model of anchoring and implantation of murine blastocysts as well as by analyzing the migration abilityof the human trophoblast cell line Swan 71. BP3 concentrations used in our experiments were: a) 2 μg/L, based on the predicted no-effect concentration for aquatic environments; b) 20 μg/L, similar to the levels of BP3 detected in serum and amniotic uid of pregnant mice exposed to BP3 from gestation day 0 to gd6 by dermal exposure; and c) BP3-200 μg/L, based on plasma concentrations reached by BP3 after a whole body topical application of sunscreens in humans. We found that BP3 negatively affected critical parameters of both in vitro implantation and early embryo development and reduced trophoblast migration through the androgen receptor (AR) pathway. Based on these ndings, it can be concluded that BP3 can affect early in vitro embryo implantation and trophoblast migration capacity.
publishDate 2023
dc.date.none.fl_str_mv 2023-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/253476
Abud, Julián Elías; Pagotto, Romina María del Luján; Galliani, Valentina; Teglia, Carla Mariela; Culzoni, Maria Julia; et al.; In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3); Springer; Research Square; 8-2023; 1-24
2693-5015
CONICET Digital
CONICET
url http://hdl.handle.net/11336/253476
identifier_str_mv Abud, Julián Elías; Pagotto, Romina María del Luján; Galliani, Valentina; Teglia, Carla Mariela; Culzoni, Maria Julia; et al.; In vitro blastocyst implantation and trophoblast migration are disrupted by the UV filter benzophenone-3 (BP3); Springer; Research Square; 8-2023; 1-24
2693-5015
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-3210668/v1
info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-3210668/v1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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