Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat

Autores
Nievas, Hernán Dario; Aurnague, Camila; Helman, María Elisa; Iza, Raúl Emilio; Costa, Magdalena; Mounsay, Oliver; Avison, Matthew; Galli, Lucía; Moredo, Fabiana Alicia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The occurrence of Escherichia coli resistant to high-priority critically important antimicrobials(HPCIA) in the food chain is a growing concern for food safety and public health. This study aimedto evaluate whether HPCIA-resistant E. coli isolated from pork and chicken meat at retail markets inLa Plata, Buenos Aires, Argentina, exhibit source-associated genomic differentiation through wholegenome sequencing. The isolates displayed a polyclonal population structure, encompassingmultiple phylogenetic groups and sequence types. Virulence gene profiles were highly diverse, withchicken-derived isolates harbouring a substantially higher number of virulence genes than porkisolates. Notably, one pork isolate carried a complete set of virulence genes characteristic ofdiarrheagenic E. coli. single Nucleotide Polymorphism-based phylogenetic analysis revealed severalclosely related subclusters, including strains recovered from both pork and chicken meat from thesame retail markets, suggesting recent clonal sharing or cross-contamination at the point of sale.These findings highlight the circulation of genetically diverse HPCIA-resistant E. coli in retail meat,underscoring the potential public health risk and the importance of monitoring resistance andvirulence determinants throughout the food production chain.
Fil: Nievas, Hernán Dario. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Aurnague, Camila. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Helman, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Iza, Raúl Emilio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Mounsay, Oliver. University Of Bristol;
Fil: Avison, Matthew. University Of Bristol;
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Materia
ExPEC
virulence factor genes
chicken and pork meat
ESBL
CTX-M-2
CTX-M-55
ST101
ST117
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/286961
Acceder
id CONICETDig_d75d66980711091c41cd8d0b05a378dd
oai_identifier_str oai:ri.conicet.gov.ar:11336/286961
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meatNievas, Hernán DarioAurnague, CamilaHelman, María ElisaIza, Raúl EmilioCosta, MagdalenaMounsay, OliverAvison, MatthewGalli, LucíaMoredo, Fabiana AliciaExPECvirulence factor geneschicken and pork meatESBLCTX-M-2CTX-M-55ST101ST117https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The occurrence of Escherichia coli resistant to high-priority critically important antimicrobials(HPCIA) in the food chain is a growing concern for food safety and public health. This study aimedto evaluate whether HPCIA-resistant E. coli isolated from pork and chicken meat at retail markets inLa Plata, Buenos Aires, Argentina, exhibit source-associated genomic differentiation through wholegenome sequencing. The isolates displayed a polyclonal population structure, encompassingmultiple phylogenetic groups and sequence types. Virulence gene profiles were highly diverse, withchicken-derived isolates harbouring a substantially higher number of virulence genes than porkisolates. Notably, one pork isolate carried a complete set of virulence genes characteristic ofdiarrheagenic E. coli. single Nucleotide Polymorphism-based phylogenetic analysis revealed severalclosely related subclusters, including strains recovered from both pork and chicken meat from thesame retail markets, suggesting recent clonal sharing or cross-contamination at the point of sale.These findings highlight the circulation of genetically diverse HPCIA-resistant E. coli in retail meat,underscoring the potential public health risk and the importance of monitoring resistance andvirulence determinants throughout the food production chain.Fil: Nievas, Hernán Dario. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Aurnague, Camila. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Helman, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Iza, Raúl Emilio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Mounsay, Oliver. University Of Bristol;Fil: Avison, Matthew. University Of Bristol;Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaMDPI2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/286961Nievas, Hernán Dario; Aurnague, Camila; Helman, María Elisa; Iza, Raúl Emilio; Costa, Magdalena; et al.; Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat; MDPI; Pathogens; 4-2026; 1-182076-0817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens15040438info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-17T09:39:00Zoai:ri.conicet.gov.ar:11336/286961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-17 09:39:00.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
title Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
spellingShingle Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
Nievas, Hernán Dario
ExPEC
virulence factor genes
chicken and pork meat
ESBL
CTX-M-2
CTX-M-55
ST101
ST117
title_short Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
title_full Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
title_fullStr Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
title_full_unstemmed Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
title_sort Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat
dc.creator.none.fl_str_mv Nievas, Hernán Dario
Aurnague, Camila
Helman, María Elisa
Iza, Raúl Emilio
Costa, Magdalena
Mounsay, Oliver
Avison, Matthew
Galli, Lucía
Moredo, Fabiana Alicia
author Nievas, Hernán Dario
author_facet Nievas, Hernán Dario
Aurnague, Camila
Helman, María Elisa
Iza, Raúl Emilio
Costa, Magdalena
Mounsay, Oliver
Avison, Matthew
Galli, Lucía
Moredo, Fabiana Alicia
author_role author
author2 Aurnague, Camila
Helman, María Elisa
Iza, Raúl Emilio
Costa, Magdalena
Mounsay, Oliver
Avison, Matthew
Galli, Lucía
Moredo, Fabiana Alicia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ExPEC
virulence factor genes
chicken and pork meat
ESBL
CTX-M-2
CTX-M-55
ST101
ST117
topic ExPEC
virulence factor genes
chicken and pork meat
ESBL
CTX-M-2
CTX-M-55
ST101
ST117
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The occurrence of Escherichia coli resistant to high-priority critically important antimicrobials(HPCIA) in the food chain is a growing concern for food safety and public health. This study aimedto evaluate whether HPCIA-resistant E. coli isolated from pork and chicken meat at retail markets inLa Plata, Buenos Aires, Argentina, exhibit source-associated genomic differentiation through wholegenome sequencing. The isolates displayed a polyclonal population structure, encompassingmultiple phylogenetic groups and sequence types. Virulence gene profiles were highly diverse, withchicken-derived isolates harbouring a substantially higher number of virulence genes than porkisolates. Notably, one pork isolate carried a complete set of virulence genes characteristic ofdiarrheagenic E. coli. single Nucleotide Polymorphism-based phylogenetic analysis revealed severalclosely related subclusters, including strains recovered from both pork and chicken meat from thesame retail markets, suggesting recent clonal sharing or cross-contamination at the point of sale.These findings highlight the circulation of genetically diverse HPCIA-resistant E. coli in retail meat,underscoring the potential public health risk and the importance of monitoring resistance andvirulence determinants throughout the food production chain.
Fil: Nievas, Hernán Dario. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Aurnague, Camila. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Helman, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Iza, Raúl Emilio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Mounsay, Oliver. University Of Bristol;
Fil: Avison, Matthew. University Of Bristol;
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
description The occurrence of Escherichia coli resistant to high-priority critically important antimicrobials(HPCIA) in the food chain is a growing concern for food safety and public health. This study aimedto evaluate whether HPCIA-resistant E. coli isolated from pork and chicken meat at retail markets inLa Plata, Buenos Aires, Argentina, exhibit source-associated genomic differentiation through wholegenome sequencing. The isolates displayed a polyclonal population structure, encompassingmultiple phylogenetic groups and sequence types. Virulence gene profiles were highly diverse, withchicken-derived isolates harbouring a substantially higher number of virulence genes than porkisolates. Notably, one pork isolate carried a complete set of virulence genes characteristic ofdiarrheagenic E. coli. single Nucleotide Polymorphism-based phylogenetic analysis revealed severalclosely related subclusters, including strains recovered from both pork and chicken meat from thesame retail markets, suggesting recent clonal sharing or cross-contamination at the point of sale.These findings highlight the circulation of genetically diverse HPCIA-resistant E. coli in retail meat,underscoring the potential public health risk and the importance of monitoring resistance andvirulence determinants throughout the food production chain.
publishDate 2026
dc.date.none.fl_str_mv 2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/286961
Nievas, Hernán Dario; Aurnague, Camila; Helman, María Elisa; Iza, Raúl Emilio; Costa, Magdalena; et al.; Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat; MDPI; Pathogens; 4-2026; 1-18
2076-0817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/286961
identifier_str_mv Nievas, Hernán Dario; Aurnague, Camila; Helman, María Elisa; Iza, Raúl Emilio; Costa, Magdalena; et al.; Genomic diversity and virulence potential of high-priority critically important antimicrobial-resistant Escherichia coli from pork and chicken retail meat; MDPI; Pathogens; 4-2026; 1-18
2076-0817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens15040438
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1868338923847024640
score 13.040872

Publicaciones similares