Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples

Autores
Marin Barroso, Evelyn del Valle; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Pereira, Sirley Vanesa
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The gluten-free diet is a unique, effective treatment for different conditions related to gluten consumption. Therefore, it is crucial the availability of new methodologies for the sensitive and specific determination of gluten content in food samples. Herein, a screen printed electrode modified with carbon nanofibers coupled to a paper immunoaffinity platform was reported for the determination of gliadin in foods samples. The paper microzone covalently functionalized with specific anti-gliadin antibodies was placed on the modified electrode surface for the electrochemical determination of gliadin. The surface of the electrode modified with carbon nanofibers was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), which showed the improved sensitivity of the modified surface. The developed device was evaluated using different flour samples obtaining a favorable response. The calculated limit of detection for the device in analyzed samples was 0.005 mg kg -1 and for the enzyme-linked immunosorbent assay was 1.5 mg kg -1 . The coefficient of variation (CV) for the determination of 20 μg kg -1 of gliadin was 4.11%. The disposable electrochemical sensor developed, represents an easy-to-use and low-cost strategy for the determination of gliadin in food samples.
Fil: Marin Barroso, Evelyn del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Materia
GLIADIN
IMMUNOSENSOR
ELECTROCHEMISTRY
CARBON NANOFIBER
FOOD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105171
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/105171
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samplesMarin Barroso, Evelyn del ValleMessina, Germán AlejandroBertolino, Franco AdriánRaba, JulioPereira, Sirley VanesaGLIADINIMMUNOSENSORELECTROCHEMISTRYCARBON NANOFIBERFOODhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The gluten-free diet is a unique, effective treatment for different conditions related to gluten consumption. Therefore, it is crucial the availability of new methodologies for the sensitive and specific determination of gluten content in food samples. Herein, a screen printed electrode modified with carbon nanofibers coupled to a paper immunoaffinity platform was reported for the determination of gliadin in foods samples. The paper microzone covalently functionalized with specific anti-gliadin antibodies was placed on the modified electrode surface for the electrochemical determination of gliadin. The surface of the electrode modified with carbon nanofibers was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), which showed the improved sensitivity of the modified surface. The developed device was evaluated using different flour samples obtaining a favorable response. The calculated limit of detection for the device in analyzed samples was 0.005 mg kg -1 and for the enzyme-linked immunosorbent assay was 1.5 mg kg -1 . The coefficient of variation (CV) for the determination of 20 μg kg -1 of gliadin was 4.11%. The disposable electrochemical sensor developed, represents an easy-to-use and low-cost strategy for the determination of gliadin in food samples.Fil: Marin Barroso, Evelyn del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaFil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaFil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; ArgentinaRoyal Society of Chemistry2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105171Marin Barroso, Evelyn del Valle; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Pereira, Sirley Vanesa; Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples; Royal Society of Chemistry; Analytical Methods; 11; 16; 4-2019; 2170-21781759-9660CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2019/AY/C9AY00255Cinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C9AY00255Cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:44Zoai:ri.conicet.gov.ar:11336/105171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:44.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
title Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
spellingShingle Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
Marin Barroso, Evelyn del Valle
GLIADIN
IMMUNOSENSOR
ELECTROCHEMISTRY
CARBON NANOFIBER
FOOD
title_short Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
title_full Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
title_fullStr Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
title_full_unstemmed Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
title_sort Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
dc.creator.none.fl_str_mv Marin Barroso, Evelyn del Valle
Messina, Germán Alejandro
Bertolino, Franco Adrián
Raba, Julio
Pereira, Sirley Vanesa
author Marin Barroso, Evelyn del Valle
author_facet Marin Barroso, Evelyn del Valle
Messina, Germán Alejandro
Bertolino, Franco Adrián
Raba, Julio
Pereira, Sirley Vanesa
author_role author
author2 Messina, Germán Alejandro
Bertolino, Franco Adrián
Raba, Julio
Pereira, Sirley Vanesa
author2_role author
author
author
author
dc.subject.none.fl_str_mv GLIADIN
IMMUNOSENSOR
ELECTROCHEMISTRY
CARBON NANOFIBER
FOOD
topic GLIADIN
IMMUNOSENSOR
ELECTROCHEMISTRY
CARBON NANOFIBER
FOOD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The gluten-free diet is a unique, effective treatment for different conditions related to gluten consumption. Therefore, it is crucial the availability of new methodologies for the sensitive and specific determination of gluten content in food samples. Herein, a screen printed electrode modified with carbon nanofibers coupled to a paper immunoaffinity platform was reported for the determination of gliadin in foods samples. The paper microzone covalently functionalized with specific anti-gliadin antibodies was placed on the modified electrode surface for the electrochemical determination of gliadin. The surface of the electrode modified with carbon nanofibers was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), which showed the improved sensitivity of the modified surface. The developed device was evaluated using different flour samples obtaining a favorable response. The calculated limit of detection for the device in analyzed samples was 0.005 mg kg -1 and for the enzyme-linked immunosorbent assay was 1.5 mg kg -1 . The coefficient of variation (CV) for the determination of 20 μg kg -1 of gliadin was 4.11%. The disposable electrochemical sensor developed, represents an easy-to-use and low-cost strategy for the determination of gliadin in food samples.
Fil: Marin Barroso, Evelyn del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
description The gluten-free diet is a unique, effective treatment for different conditions related to gluten consumption. Therefore, it is crucial the availability of new methodologies for the sensitive and specific determination of gluten content in food samples. Herein, a screen printed electrode modified with carbon nanofibers coupled to a paper immunoaffinity platform was reported for the determination of gliadin in foods samples. The paper microzone covalently functionalized with specific anti-gliadin antibodies was placed on the modified electrode surface for the electrochemical determination of gliadin. The surface of the electrode modified with carbon nanofibers was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), which showed the improved sensitivity of the modified surface. The developed device was evaluated using different flour samples obtaining a favorable response. The calculated limit of detection for the device in analyzed samples was 0.005 mg kg -1 and for the enzyme-linked immunosorbent assay was 1.5 mg kg -1 . The coefficient of variation (CV) for the determination of 20 μg kg -1 of gliadin was 4.11%. The disposable electrochemical sensor developed, represents an easy-to-use and low-cost strategy for the determination of gliadin in food samples.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105171
Marin Barroso, Evelyn del Valle; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Pereira, Sirley Vanesa; Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples; Royal Society of Chemistry; Analytical Methods; 11; 16; 4-2019; 2170-2178
1759-9660
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105171
identifier_str_mv Marin Barroso, Evelyn del Valle; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Pereira, Sirley Vanesa; Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples; Royal Society of Chemistry; Analytical Methods; 11; 16; 4-2019; 2170-2178
1759-9660
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2019/AY/C9AY00255C
info:eu-repo/semantics/altIdentifier/doi/10.1039/C9AY00255C
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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