Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation

Autores
Pizarro, María Dolores; Mediavilla, Maria Gabriela; Berardi, Florencia; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; Mamprin, María Eugenia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: This work focuses on ammonia metabolism of Liver Microorgans (LMOs) after cold preservation in a normothermic reoxygenation system (NRS). We have previously reported the development of a novel preservation solution, Bes-Gluconate-PEG 35 kDa (BG35) that showed the same efficacy as ViaSpan® to protect LMOs against cold preservation injury. The objective of this work was to study mRNA levels and activities of two key Urea Cycle enzymes, Carbamyl Phosphate Synthetase I (CPSI) and Ornithine Transcar-bamylase (OTC), after preservation of LMOs in BG35 and ViaSpan® and the ability of these tissue slices to detoxify an ammonia overload in a NRS model. Material and methods: After 48 h of cold storage (0°C in BG35 or ViaSpan®) LMOs were rewarmed in KHR containing an ammonium chloride overload (1 mM). We determined ammonium detoxification capacity (ADC), urea synthesis and enzyme activities and relative mRNA levels for CPSI and OTC. Results: At the end of reoxygenation LMOs cold preserved in BG35 have ADC and urea synthesis similar to controls. ViaSpan® group demonstrated a lower capacity to detoxify ammonia and to synthesize urea than fresh LMOs during the whole reoxygenation period which correlated with the lower mRNA levels and activities for CPSI and OTC observed for this group. Conclusion: We demonstrate that our preservation conditions (48 hours, BG35 solution, anoxia, 0oC) did not affect ammonia metabolism of cold preserved LMOs maintaining the physiological and biochemical liver functions tested, which allows their future use as biological component of a BAL system.
Fil: Pizarro, María Dolores. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina
Fil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Berardi, Florencia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina
Fil: Tiribelli, Claudio. Universidad de Trieste. Centro Studi Fegato; Italia
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina
Fil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Cs.fisiologicas. Area Farmacologia; Argentina
Materia
Ammonia Metabolism
Liver Microorgans
Cold Storage
Bg35
Preservation Solutions
Liver Preservation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8812
Acceder
id CONICETDig_d67ff41b8b1f8280b30740e9b27003d9
oai_identifier_str oai:ri.conicet.gov.ar:11336/8812
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenationPizarro, María DoloresMediavilla, Maria GabrielaBerardi, FlorenciaTiribelli, ClaudioRodriguez, Joaquin ValentinMamprin, María EugeniaAmmonia MetabolismLiver MicroorgansCold StorageBg35Preservation SolutionsLiver Preservationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Introduction: This work focuses on ammonia metabolism of Liver Microorgans (LMOs) after cold preservation in a normothermic reoxygenation system (NRS). We have previously reported the development of a novel preservation solution, Bes-Gluconate-PEG 35 kDa (BG35) that showed the same efficacy as ViaSpan® to protect LMOs against cold preservation injury. The objective of this work was to study mRNA levels and activities of two key Urea Cycle enzymes, Carbamyl Phosphate Synthetase I (CPSI) and Ornithine Transcar-bamylase (OTC), after preservation of LMOs in BG35 and ViaSpan® and the ability of these tissue slices to detoxify an ammonia overload in a NRS model. Material and methods: After 48 h of cold storage (0°C in BG35 or ViaSpan®) LMOs were rewarmed in KHR containing an ammonium chloride overload (1 mM). We determined ammonium detoxification capacity (ADC), urea synthesis and enzyme activities and relative mRNA levels for CPSI and OTC. Results: At the end of reoxygenation LMOs cold preserved in BG35 have ADC and urea synthesis similar to controls. ViaSpan® group demonstrated a lower capacity to detoxify ammonia and to synthesize urea than fresh LMOs during the whole reoxygenation period which correlated with the lower mRNA levels and activities for CPSI and OTC observed for this group. Conclusion: We demonstrate that our preservation conditions (48 hours, BG35 solution, anoxia, 0oC) did not affect ammonia metabolism of cold preserved LMOs maintaining the physiological and biochemical liver functions tested, which allows their future use as biological component of a BAL system.Fil: Pizarro, María Dolores. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; ArgentinaFil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Berardi, Florencia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; ArgentinaFil: Tiribelli, Claudio. Universidad de Trieste. Centro Studi Fegato; ItaliaFil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; ArgentinaFil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Cs.fisiologicas. Area Farmacologia; ArgentinaMexican Assoc Hepatology2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8812Pizarro, María Dolores; Mediavilla, Maria Gabriela; Berardi, Florencia; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation; Mexican Assoc Hepatology; Annals Of Hepatology; 13; 2; 3-2014; 256-2641665-2681enginfo:eu-repo/semantics/altIdentifier/url/https://www.elsevier.es/en-revista-annals-hepatology-16-articulo-cold-storage-liver-microorgans-in-S1665268119308890info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:29:23Zoai:ri.conicet.gov.ar:11336/8812instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:29:24.298CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
title Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
spellingShingle Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
Pizarro, María Dolores
Ammonia Metabolism
Liver Microorgans
Cold Storage
Bg35
Preservation Solutions
Liver Preservation
title_short Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
title_full Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
title_fullStr Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
title_full_unstemmed Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
title_sort Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation
dc.creator.none.fl_str_mv Pizarro, María Dolores
Mediavilla, Maria Gabriela
Berardi, Florencia
Tiribelli, Claudio
Rodriguez, Joaquin Valentin
Mamprin, María Eugenia
author Pizarro, María Dolores
author_facet Pizarro, María Dolores
Mediavilla, Maria Gabriela
Berardi, Florencia
Tiribelli, Claudio
Rodriguez, Joaquin Valentin
Mamprin, María Eugenia
author_role author
author2 Mediavilla, Maria Gabriela
Berardi, Florencia
Tiribelli, Claudio
Rodriguez, Joaquin Valentin
Mamprin, María Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ammonia Metabolism
Liver Microorgans
Cold Storage
Bg35
Preservation Solutions
Liver Preservation
topic Ammonia Metabolism
Liver Microorgans
Cold Storage
Bg35
Preservation Solutions
Liver Preservation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: This work focuses on ammonia metabolism of Liver Microorgans (LMOs) after cold preservation in a normothermic reoxygenation system (NRS). We have previously reported the development of a novel preservation solution, Bes-Gluconate-PEG 35 kDa (BG35) that showed the same efficacy as ViaSpan® to protect LMOs against cold preservation injury. The objective of this work was to study mRNA levels and activities of two key Urea Cycle enzymes, Carbamyl Phosphate Synthetase I (CPSI) and Ornithine Transcar-bamylase (OTC), after preservation of LMOs in BG35 and ViaSpan® and the ability of these tissue slices to detoxify an ammonia overload in a NRS model. Material and methods: After 48 h of cold storage (0°C in BG35 or ViaSpan®) LMOs were rewarmed in KHR containing an ammonium chloride overload (1 mM). We determined ammonium detoxification capacity (ADC), urea synthesis and enzyme activities and relative mRNA levels for CPSI and OTC. Results: At the end of reoxygenation LMOs cold preserved in BG35 have ADC and urea synthesis similar to controls. ViaSpan® group demonstrated a lower capacity to detoxify ammonia and to synthesize urea than fresh LMOs during the whole reoxygenation period which correlated with the lower mRNA levels and activities for CPSI and OTC observed for this group. Conclusion: We demonstrate that our preservation conditions (48 hours, BG35 solution, anoxia, 0oC) did not affect ammonia metabolism of cold preserved LMOs maintaining the physiological and biochemical liver functions tested, which allows their future use as biological component of a BAL system.
Fil: Pizarro, María Dolores. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina
Fil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Berardi, Florencia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina
Fil: Tiribelli, Claudio. Universidad de Trieste. Centro Studi Fegato; Italia
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliâ­nica y Aplicada; Argentina
Fil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Cs.fisiologicas. Area Farmacologia; Argentina
description Introduction: This work focuses on ammonia metabolism of Liver Microorgans (LMOs) after cold preservation in a normothermic reoxygenation system (NRS). We have previously reported the development of a novel preservation solution, Bes-Gluconate-PEG 35 kDa (BG35) that showed the same efficacy as ViaSpan® to protect LMOs against cold preservation injury. The objective of this work was to study mRNA levels and activities of two key Urea Cycle enzymes, Carbamyl Phosphate Synthetase I (CPSI) and Ornithine Transcar-bamylase (OTC), after preservation of LMOs in BG35 and ViaSpan® and the ability of these tissue slices to detoxify an ammonia overload in a NRS model. Material and methods: After 48 h of cold storage (0°C in BG35 or ViaSpan®) LMOs were rewarmed in KHR containing an ammonium chloride overload (1 mM). We determined ammonium detoxification capacity (ADC), urea synthesis and enzyme activities and relative mRNA levels for CPSI and OTC. Results: At the end of reoxygenation LMOs cold preserved in BG35 have ADC and urea synthesis similar to controls. ViaSpan® group demonstrated a lower capacity to detoxify ammonia and to synthesize urea than fresh LMOs during the whole reoxygenation period which correlated with the lower mRNA levels and activities for CPSI and OTC observed for this group. Conclusion: We demonstrate that our preservation conditions (48 hours, BG35 solution, anoxia, 0oC) did not affect ammonia metabolism of cold preserved LMOs maintaining the physiological and biochemical liver functions tested, which allows their future use as biological component of a BAL system.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8812
Pizarro, María Dolores; Mediavilla, Maria Gabriela; Berardi, Florencia; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation; Mexican Assoc Hepatology; Annals Of Hepatology; 13; 2; 3-2014; 256-264
1665-2681
url http://hdl.handle.net/11336/8812
identifier_str_mv Pizarro, María Dolores; Mediavilla, Maria Gabriela; Berardi, Florencia; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation; Mexican Assoc Hepatology; Annals Of Hepatology; 13; 2; 3-2014; 256-264
1665-2681
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.elsevier.es/en-revista-annals-hepatology-16-articulo-cold-storage-liver-microorgans-in-S1665268119308890
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mexican Assoc Hepatology
publisher.none.fl_str_mv Mexican Assoc Hepatology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082764831457280
score 13.22299

Publicaciones similares