The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action

Autores
Colo, Georgina Pamela; Rubio, María Fernanda; Nojek Barbieri, Ignacio Martin; Werbajh, Santiago Enrique; Echeverria, Pablo Christian; Alvarado, Cecilia Viviana; Nahmod, Victor Elias; Galigniana, Mario Daniel; Costas, Monica Alejandra
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The p160 nuclear receptor coactivators represent a family of molecules, which are recruited by steroid nuclear receptors as well as other transcription factors that are over-expressed in several tumors. We investigated the role of one member of this family on the sensitivity of cells to apoptosis. We observed that over-expression of the RAC3 p160 coactivator inhibits hydrogen peroxide-induced cell death in HEK293 cells. The mechanism involves the activation of anti-apoptotic pathways mediated through enhanced NF-kB activity, inhibition of caspase-9 activation, diminished AIF nuclear localization and the change in the activation pattern of several kinases, including the increase in both AKT and p38 kinases activities, and inhibition of ERK2. Moreover, RAC3 has been found associated to a protein complex containing AIF, Hsp90 and dynein, suggesting a role for the coactivator in the cytoplasmatic-nuclear-transport of these proteins associated to cytoskeleton. These results demonstrate that there are several molecular pathways that could be affected by their over-expression, including those not restricted to steroid regulation or the nuclear action of coactivators, which results in diminished sensitivity to apoptosis. Furthermore, this could represent one mechanism by which coactivators contribute to tumor development.
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Rubio, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Nojek Barbieri, Ignacio Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Werbajh, Santiago Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Echeverria, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Alvarado, Cecilia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Nahmod, Victor Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Galigniana, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Materia
APOPTOSIS
RAC3
COACTIVADORES
NF-KB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104934
Acceder
id CONICETDig_d611affe3c368c4bfb2881a08fee6564
oai_identifier_str oai:ri.conicet.gov.ar:11336/104934
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic actionColo, Georgina PamelaRubio, María FernandaNojek Barbieri, Ignacio MartinWerbajh, Santiago EnriqueEcheverria, Pablo ChristianAlvarado, Cecilia VivianaNahmod, Victor EliasGaligniana, Mario DanielCostas, Monica AlejandraAPOPTOSISRAC3COACTIVADORESNF-KBhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3The p160 nuclear receptor coactivators represent a family of molecules, which are recruited by steroid nuclear receptors as well as other transcription factors that are over-expressed in several tumors. We investigated the role of one member of this family on the sensitivity of cells to apoptosis. We observed that over-expression of the RAC3 p160 coactivator inhibits hydrogen peroxide-induced cell death in HEK293 cells. The mechanism involves the activation of anti-apoptotic pathways mediated through enhanced NF-kB activity, inhibition of caspase-9 activation, diminished AIF nuclear localization and the change in the activation pattern of several kinases, including the increase in both AKT and p38 kinases activities, and inhibition of ERK2. Moreover, RAC3 has been found associated to a protein complex containing AIF, Hsp90 and dynein, suggesting a role for the coactivator in the cytoplasmatic-nuclear-transport of these proteins associated to cytoskeleton. These results demonstrate that there are several molecular pathways that could be affected by their over-expression, including those not restricted to steroid regulation or the nuclear action of coactivators, which results in diminished sensitivity to apoptosis. Furthermore, this could represent one mechanism by which coactivators contribute to tumor development.Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Rubio, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Nojek Barbieri, Ignacio Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Werbajh, Santiago Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Echeverria, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Alvarado, Cecilia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Nahmod, Victor Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Galigniana, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaNature Publishing Group2007-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104934Colo, Georgina Pamela; Rubio, María Fernanda; Nojek Barbieri, Ignacio Martin; Werbajh, Santiago Enrique; Echeverria, Pablo Christian; et al.; The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action; Nature Publishing Group; Oncogene; 27; 17; 10-2007; 2430-24440950-9232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/1210900info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.onc.1210900info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:45Zoai:ri.conicet.gov.ar:11336/104934instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:45.821CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
title The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
spellingShingle The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
Colo, Georgina Pamela
APOPTOSIS
RAC3
COACTIVADORES
NF-KB
title_short The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
title_full The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
title_fullStr The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
title_full_unstemmed The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
title_sort The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action
dc.creator.none.fl_str_mv Colo, Georgina Pamela
Rubio, María Fernanda
Nojek Barbieri, Ignacio Martin
Werbajh, Santiago Enrique
Echeverria, Pablo Christian
Alvarado, Cecilia Viviana
Nahmod, Victor Elias
Galigniana, Mario Daniel
Costas, Monica Alejandra
author Colo, Georgina Pamela
author_facet Colo, Georgina Pamela
Rubio, María Fernanda
Nojek Barbieri, Ignacio Martin
Werbajh, Santiago Enrique
Echeverria, Pablo Christian
Alvarado, Cecilia Viviana
Nahmod, Victor Elias
Galigniana, Mario Daniel
Costas, Monica Alejandra
author_role author
author2 Rubio, María Fernanda
Nojek Barbieri, Ignacio Martin
Werbajh, Santiago Enrique
Echeverria, Pablo Christian
Alvarado, Cecilia Viviana
Nahmod, Victor Elias
Galigniana, Mario Daniel
Costas, Monica Alejandra
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
RAC3
COACTIVADORES
NF-KB
topic APOPTOSIS
RAC3
COACTIVADORES
NF-KB
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The p160 nuclear receptor coactivators represent a family of molecules, which are recruited by steroid nuclear receptors as well as other transcription factors that are over-expressed in several tumors. We investigated the role of one member of this family on the sensitivity of cells to apoptosis. We observed that over-expression of the RAC3 p160 coactivator inhibits hydrogen peroxide-induced cell death in HEK293 cells. The mechanism involves the activation of anti-apoptotic pathways mediated through enhanced NF-kB activity, inhibition of caspase-9 activation, diminished AIF nuclear localization and the change in the activation pattern of several kinases, including the increase in both AKT and p38 kinases activities, and inhibition of ERK2. Moreover, RAC3 has been found associated to a protein complex containing AIF, Hsp90 and dynein, suggesting a role for the coactivator in the cytoplasmatic-nuclear-transport of these proteins associated to cytoskeleton. These results demonstrate that there are several molecular pathways that could be affected by their over-expression, including those not restricted to steroid regulation or the nuclear action of coactivators, which results in diminished sensitivity to apoptosis. Furthermore, this could represent one mechanism by which coactivators contribute to tumor development.
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Rubio, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Nojek Barbieri, Ignacio Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Werbajh, Santiago Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Echeverria, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Alvarado, Cecilia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Nahmod, Victor Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Galigniana, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
description The p160 nuclear receptor coactivators represent a family of molecules, which are recruited by steroid nuclear receptors as well as other transcription factors that are over-expressed in several tumors. We investigated the role of one member of this family on the sensitivity of cells to apoptosis. We observed that over-expression of the RAC3 p160 coactivator inhibits hydrogen peroxide-induced cell death in HEK293 cells. The mechanism involves the activation of anti-apoptotic pathways mediated through enhanced NF-kB activity, inhibition of caspase-9 activation, diminished AIF nuclear localization and the change in the activation pattern of several kinases, including the increase in both AKT and p38 kinases activities, and inhibition of ERK2. Moreover, RAC3 has been found associated to a protein complex containing AIF, Hsp90 and dynein, suggesting a role for the coactivator in the cytoplasmatic-nuclear-transport of these proteins associated to cytoskeleton. These results demonstrate that there are several molecular pathways that could be affected by their over-expression, including those not restricted to steroid regulation or the nuclear action of coactivators, which results in diminished sensitivity to apoptosis. Furthermore, this could represent one mechanism by which coactivators contribute to tumor development.
publishDate 2007
dc.date.none.fl_str_mv 2007-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104934
Colo, Georgina Pamela; Rubio, María Fernanda; Nojek Barbieri, Ignacio Martin; Werbajh, Santiago Enrique; Echeverria, Pablo Christian; et al.; The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action; Nature Publishing Group; Oncogene; 27; 17; 10-2007; 2430-2444
0950-9232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104934
identifier_str_mv Colo, Georgina Pamela; Rubio, María Fernanda; Nojek Barbieri, Ignacio Martin; Werbajh, Santiago Enrique; Echeverria, Pablo Christian; et al.; The p160 nuclear receptor co-activator RAC3 exerts an anti-apoptotic role through a cytoplasmatic action; Nature Publishing Group; Oncogene; 27; 17; 10-2007; 2430-2444
0950-9232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/1210900
info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.onc.1210900
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269539375513600
score 13.13397

Publicaciones similares