Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina

Autores
Peralta, Regina Helena Saramago; Velásquez, Jorge Néstor; Cunha, Flavia de Souza; Pantano, María Laura; Sodré, Fernando Campos; Silva, Sidnei da; Astudillo, Osvaldo Germán; Peralta, José Mauro; Carnevale, Silvana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The identification and characterisation of Cryptosporidiumgenotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity ofCryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays forCryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.
Fil: Peralta, Regina Helena Saramago. Universidade Federal Fluminense; Brasil
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cunha, Flavia de Souza. Universidade Federal Fluminense; Brasil
Fil: Pantano, María Laura. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Sodré, Fernando Campos. Universidade Federal Fluminense; Brasil
Fil: Silva, Sidnei da. Fundación Oswaldo Cruz; Brasil
Fil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Peralta, José Mauro. Universidade Federal do Rio de Janeiro; Brasil
Fil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cryptosporidium
Genotypes
Gp60
Pcr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42464
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42464
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and ArgentinaPeralta, Regina Helena SaramagoVelásquez, Jorge NéstorCunha, Flavia de SouzaPantano, María LauraSodré, Fernando CamposSilva, Sidnei daAstudillo, Osvaldo GermánPeralta, José MauroCarnevale, SilvanaCryptosporidiumGenotypesGp60Pcrhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The identification and characterisation of Cryptosporidiumgenotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity ofCryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays forCryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.Fil: Peralta, Regina Helena Saramago. Universidade Federal Fluminense; BrasilFil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cunha, Flavia de Souza. Universidade Federal Fluminense; BrasilFil: Pantano, María Laura. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Sodré, Fernando Campos. Universidade Federal Fluminense; BrasilFil: Silva, Sidnei da. Fundación Oswaldo Cruz; BrasilFil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Peralta, José Mauro. Universidade Federal do Rio de Janeiro; BrasilFil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFundação Oswaldo Cruz2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42464Peralta, Regina Helena Saramago; Velásquez, Jorge Néstor; Cunha, Flavia de Souza; Pantano, María Laura; Sodré, Fernando Campos; et al.; Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 111; 1; 1-2016; 30-360074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/xpyt2jinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727433/info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760150303info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:59:22Zoai:ri.conicet.gov.ar:11336/42464instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:59:22.507CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
title Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
spellingShingle Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
Peralta, Regina Helena Saramago
Cryptosporidium
Genotypes
Gp60
Pcr
title_short Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
title_full Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
title_fullStr Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
title_full_unstemmed Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
title_sort Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
dc.creator.none.fl_str_mv Peralta, Regina Helena Saramago
Velásquez, Jorge Néstor
Cunha, Flavia de Souza
Pantano, María Laura
Sodré, Fernando Campos
Silva, Sidnei da
Astudillo, Osvaldo Germán
Peralta, José Mauro
Carnevale, Silvana
author Peralta, Regina Helena Saramago
author_facet Peralta, Regina Helena Saramago
Velásquez, Jorge Néstor
Cunha, Flavia de Souza
Pantano, María Laura
Sodré, Fernando Campos
Silva, Sidnei da
Astudillo, Osvaldo Germán
Peralta, José Mauro
Carnevale, Silvana
author_role author
author2 Velásquez, Jorge Néstor
Cunha, Flavia de Souza
Pantano, María Laura
Sodré, Fernando Campos
Silva, Sidnei da
Astudillo, Osvaldo Germán
Peralta, José Mauro
Carnevale, Silvana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cryptosporidium
Genotypes
Gp60
Pcr
topic Cryptosporidium
Genotypes
Gp60
Pcr
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The identification and characterisation of Cryptosporidiumgenotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity ofCryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays forCryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.
Fil: Peralta, Regina Helena Saramago. Universidade Federal Fluminense; Brasil
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cunha, Flavia de Souza. Universidade Federal Fluminense; Brasil
Fil: Pantano, María Laura. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Sodré, Fernando Campos. Universidade Federal Fluminense; Brasil
Fil: Silva, Sidnei da. Fundación Oswaldo Cruz; Brasil
Fil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Peralta, José Mauro. Universidade Federal do Rio de Janeiro; Brasil
Fil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The identification and characterisation of Cryptosporidiumgenotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity ofCryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays forCryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42464
Peralta, Regina Helena Saramago; Velásquez, Jorge Néstor; Cunha, Flavia de Souza; Pantano, María Laura; Sodré, Fernando Campos; et al.; Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 111; 1; 1-2016; 30-36
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42464
identifier_str_mv Peralta, Regina Helena Saramago; Velásquez, Jorge Néstor; Cunha, Flavia de Souza; Pantano, María Laura; Sodré, Fernando Campos; et al.; Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 111; 1; 1-2016; 30-36
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727433/
info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760150303
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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