Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina

• Autores: Saramago Peralta, Regina Helena; Velásquez, Jorge Néstor; Souza Cunha, Flavia de; Pantano, María Laura; Sodré, Fernando Campos; Silva, Sidnei da; Astudillo, Osvaldo Germán; Peralta, José Mauro; Carnevale, Silvana
• Año de publicación: 2016
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Memorias do instituto Oswaldo Cruz 2016; 111(1):30-36
• Materia: Adolescente; Adulto; Anciano; Argentina; Brasil; Niño; Preescolar; Cryptosporidiosis; Cryptosporidium; ADN Protozoario; ADN Ribosómico; Femenino; Variación Genética; Genotipo; Humanos; Lactante; Masculino; Persona de Mediana Edad; Polimorfismo de Longitud del Fragmento de Restricción; Análisis de Secuencia de ADN; Adulto Joven
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Saramago Peralta, Regina Helena. Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia; Brasil.
Fil: Velásquez, Jorge Néstor. Hospital Municipal de Infecciosas Dr Francisco Javier Muñiz; Argentina.
Fil: Souza Cunha, Flavia de. Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia; Brasil.
Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Campos Sodré, Fernando. Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia; Brasil.
Fil: Silva, Sidnei da. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia; Brasil.
Fil: Astudillo, Osvaldo Germán. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Peralta, José Mauro. Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof Paulo de Góes; Brasil.
Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
The identification and characterisation of Cryptosporidium genotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity of Cryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays for Cryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.