Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)

Autores: Falvo, Marianel Lucía; Albornoz Medina, Patricia; Rodrigues Juscelino; Lopez Lastra, Claudia Cristina; Juan José Garcia; Fernandes, Éverton; Luz, Christian
Año de publicación: 2018
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Ultraviolet (UV) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. While littleis known about the impact of UV on Metarhizium anisopliae Metchnikoff (Sorokin) (Hypocreales: Clavicipitaceae)conidia in aquatic mosquito-breeding sites, this study determined the effect of UV-B on the viability and virulenceof M. anisopliae sensu lato (s.l.) strain IP 46 in the laboratory against Aedes aegypti (L.) (Diptera: Culicidae) larvae.Conidia were treated in cups under defined water depths (0, 1, 2, and 3 cm) to six different UV-B doses (0, 0.657,1.971, 3.942, 7.884, 11.826, or 15.768 kJ m−2) at 27 ± 2°C. The ability of treated conidia to germinate up to 24 hpostexposure on PDAY + benomyl + chloramphenicol medium at 25 ± 1°C was adversely affected by higher dosesof UV-B radiation regardless of the water depth. Germination, however, did not fall below 70% regardless of thetest conditions. In fact, conidial virulence against second-instar larvae was not affected by either the water depth(F3,84 = 0.3, P = 0.85) or any tested levels of UV-B radiation (F6,21 ≤ 1.2, P ≥ 0.39) including those distinctly higher thanmight be expected for tropical sites. These findings strengthen previous observations that IP 46 has significantpotential for use against A. aegypti larvae, even when exposed to elevated UV-B irradiance levels in the smallbreeding sites that are common for this important vector.
Fil: Falvo, Marianel Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Albornoz Medina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rodrigues Juscelino. Universidade Federal de Goiás; Brasil
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Juan José Garcia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Fernandes, Éverton. Universidade Federal de Goiás; Brasil
Fil: Luz, Christian. Universidade Federal de Goiás; Brasil
Materia: ENTOMOPATHOGENIC FUNGUS
MOSQUITO
WATER LEVEL
UV-B TOLERANCE
GERMINATION
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/104241

Acceder

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network_name_str	CONICET Digital (CONICET)
spelling	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)Falvo, Marianel LucíaAlbornoz Medina, PatriciaRodrigues JuscelinoLopez Lastra, Claudia CristinaJuan José GarciaFernandes, ÉvertonLuz, ChristianENTOMOPATHOGENIC FUNGUSMOSQUITOWATER LEVELUV-B TOLERANCEGERMINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ultraviolet (UV) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. While littleis known about the impact of UV on Metarhizium anisopliae Metchnikoff (Sorokin) (Hypocreales: Clavicipitaceae)conidia in aquatic mosquito-breeding sites, this study determined the effect of UV-B on the viability and virulenceof M. anisopliae sensu lato (s.l.) strain IP 46 in the laboratory against Aedes aegypti (L.) (Diptera: Culicidae) larvae.Conidia were treated in cups under defined water depths (0, 1, 2, and 3 cm) to six different UV-B doses (0, 0.657,1.971, 3.942, 7.884, 11.826, or 15.768 kJ m−2) at 27 ± 2°C. The ability of treated conidia to germinate up to 24 hpostexposure on PDAY + benomyl + chloramphenicol medium at 25 ± 1°C was adversely affected by higher dosesof UV-B radiation regardless of the water depth. Germination, however, did not fall below 70% regardless of thetest conditions. In fact, conidial virulence against second-instar larvae was not affected by either the water depth(F3,84 = 0.3, P = 0.85) or any tested levels of UV-B radiation (F6,21 ≤ 1.2, P ≥ 0.39) including those distinctly higher thanmight be expected for tropical sites. These findings strengthen previous observations that IP 46 has significantpotential for use against A. aegypti larvae, even when exposed to elevated UV-B irradiance levels in the smallbreeding sites that are common for this important vector.Fil: Falvo, Marianel Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Albornoz Medina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Rodrigues Juscelino. Universidade Federal de Goiás; BrasilFil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Juan José Garcia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Fernandes, Éverton. Universidade Federal de Goiás; BrasilFil: Luz, Christian. Universidade Federal de Goiás; BrasilEntomological Society of America2018-05-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104241Falvo, Marianel Lucía; Albornoz Medina, Patricia; Rodrigues Juscelino; Lopez Lastra, Claudia Cristina; Juan José Garcia; et al.; Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae); Entomological Society of America; Journal of Medical Entomology; 55; 10-5-2018; 1330-13330022-2585CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jme/tjy071info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article-abstract/55/5/1330/4994790?redirectedFrom=fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:35Zoai:ri.conicet.gov.ar:11336/104241instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:35.271CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)
title	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)
spellingShingle	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae) Falvo, Marianel Lucía ENTOMOPATHOGENIC FUNGUS MOSQUITO WATER LEVEL UV-B TOLERANCE GERMINATION
title_short	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)
title_full	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)
title_fullStr	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)
title_full_unstemmed	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)
title_sort	Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)
dc.creator.none.fl_str_mv	Falvo, Marianel Lucía Albornoz Medina, Patricia Rodrigues Juscelino Lopez Lastra, Claudia Cristina Juan José Garcia Fernandes, Éverton Luz, Christian
author	Falvo, Marianel Lucía
author_facet	Falvo, Marianel Lucía Albornoz Medina, Patricia Rodrigues Juscelino Lopez Lastra, Claudia Cristina Juan José Garcia Fernandes, Éverton Luz, Christian
author_role	author
author2	Albornoz Medina, Patricia Rodrigues Juscelino Lopez Lastra, Claudia Cristina Juan José Garcia Fernandes, Éverton Luz, Christian
author2_role	author author author author author author
dc.subject.none.fl_str_mv	ENTOMOPATHOGENIC FUNGUS MOSQUITO WATER LEVEL UV-B TOLERANCE GERMINATION
topic	ENTOMOPATHOGENIC FUNGUS MOSQUITO WATER LEVEL UV-B TOLERANCE GERMINATION
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Ultraviolet (UV) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. While littleis known about the impact of UV on Metarhizium anisopliae Metchnikoff (Sorokin) (Hypocreales: Clavicipitaceae)conidia in aquatic mosquito-breeding sites, this study determined the effect of UV-B on the viability and virulenceof M. anisopliae sensu lato (s.l.) strain IP 46 in the laboratory against Aedes aegypti (L.) (Diptera: Culicidae) larvae.Conidia were treated in cups under defined water depths (0, 1, 2, and 3 cm) to six different UV-B doses (0, 0.657,1.971, 3.942, 7.884, 11.826, or 15.768 kJ m−2) at 27 ± 2°C. The ability of treated conidia to germinate up to 24 hpostexposure on PDAY + benomyl + chloramphenicol medium at 25 ± 1°C was adversely affected by higher dosesof UV-B radiation regardless of the water depth. Germination, however, did not fall below 70% regardless of thetest conditions. In fact, conidial virulence against second-instar larvae was not affected by either the water depth(F3,84 = 0.3, P = 0.85) or any tested levels of UV-B radiation (F6,21 ≤ 1.2, P ≥ 0.39) including those distinctly higher thanmight be expected for tropical sites. These findings strengthen previous observations that IP 46 has significantpotential for use against A. aegypti larvae, even when exposed to elevated UV-B irradiance levels in the smallbreeding sites that are common for this important vector. Fil: Falvo, Marianel Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Albornoz Medina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Rodrigues Juscelino. Universidade Federal de Goiás; Brasil Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Juan José Garcia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Fernandes, Éverton. Universidade Federal de Goiás; Brasil Fil: Luz, Christian. Universidade Federal de Goiás; Brasil
description	Ultraviolet (UV) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. While littleis known about the impact of UV on Metarhizium anisopliae Metchnikoff (Sorokin) (Hypocreales: Clavicipitaceae)conidia in aquatic mosquito-breeding sites, this study determined the effect of UV-B on the viability and virulenceof M. anisopliae sensu lato (s.l.) strain IP 46 in the laboratory against Aedes aegypti (L.) (Diptera: Culicidae) larvae.Conidia were treated in cups under defined water depths (0, 1, 2, and 3 cm) to six different UV-B doses (0, 0.657,1.971, 3.942, 7.884, 11.826, or 15.768 kJ m−2) at 27 ± 2°C. The ability of treated conidia to germinate up to 24 hpostexposure on PDAY + benomyl + chloramphenicol medium at 25 ± 1°C was adversely affected by higher dosesof UV-B radiation regardless of the water depth. Germination, however, did not fall below 70% regardless of thetest conditions. In fact, conidial virulence against second-instar larvae was not affected by either the water depth(F3,84 = 0.3, P = 0.85) or any tested levels of UV-B radiation (F6,21 ≤ 1.2, P ≥ 0.39) including those distinctly higher thanmight be expected for tropical sites. These findings strengthen previous observations that IP 46 has significantpotential for use against A. aegypti larvae, even when exposed to elevated UV-B irradiance levels in the smallbreeding sites that are common for this important vector.
publishDate	2018
dc.date.none.fl_str_mv	2018-05-10
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/104241 Falvo, Marianel Lucía; Albornoz Medina, Patricia; Rodrigues Juscelino; Lopez Lastra, Claudia Cristina; Juan José Garcia; et al.; Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae); Entomological Society of America; Journal of Medical Entomology; 55; 10-5-2018; 1330-1333 0022-2585 CONICET Digital CONICET
url	http://hdl.handle.net/11336/104241
identifier_str_mv	Falvo, Marianel Lucía; Albornoz Medina, Patricia; Rodrigues Juscelino; Lopez Lastra, Claudia Cristina; Juan José Garcia; et al.; Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae); Entomological Society of America; Journal of Medical Entomology; 55; 10-5-2018; 1330-1333 0022-2585 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1093/jme/tjy071 info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article-abstract/55/5/1330/4994790?redirectedFrom=fulltext
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Entomological Society of America
publisher.none.fl_str_mv	Entomological Society of America
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	13.13397

Effect of UV-B Irradiation on Water-Suspended Metarhizium anisopliae s.l. (Hypocreales: Clavicipitaceae) Conidia and Their Larvicidal Activity in Aedes aegypti (Diptera: Culicidae)

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