New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)

Autores
Gutierrez, Alejandra Concepción; Machado, J. A. R.; Hubner Campos, R.; Pennisi, M. A.; Rodrigues, J.; Lopez Lastra, Claudia Cristina; Garcia, Juan Jose; Fernandes, É. K. K.; Luz, C.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To study the marked resistance of Periplaneta americana to entomopathogenic Metarhizium anisopliae. Methods and Results: The low susceptibility of 4th instar nymphs applied topically with conidia seemed to be related to an active removal of conidia by the cockroach and to a disabled or retarded germination and subsequent development of conidia on the cuticle (up to 80% germination in the next 7 days after application). Inhibitions or delays of germination were related to the composition of the epicuticular fatty acids (30·1% w/w oleic, 28·3% w/w linoleic, 24·5% w/w palmitic and 11·7% w/w stearic acid) reported here. Propagules invading the nymphs through the cuticle took at least 3 days to reach the haemocoel, and no propagules were found after day 8 post-treatment. Strain IP 46 infected >50% of nymphs treated with doses ≥2 × 104 hyphal bodies (HB) nymph−1 and reduced the survival of nymphs ≤50%. Most nymphs (>70%) survived after injection of 6 × 103 and 2 × 103 HB nymph−1. Conclusions: Findings emphasize a distinct resistance of nymphs of the American cockroach to infections by M. anisopliae. Significance and Impact of the Study: Our findings provide support for the development of biological control of this synanthropic cockroach pest.
Fil: Gutierrez, Alejandra Concepción. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Machado, J. A. R.. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Hubner Campos, R.. Universidade Federal de Goiás; Brasil
Fil: Pennisi, M. A.. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Rodrigues, J.. Universidade Federal de Goiás; Brasil
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Garcia, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Fernandes, É. K. K.. Universidade Federal de Goiás; Brasil
Fil: Luz, C.. Universidade Federal de Goiás; Brasil
Materia
Behaviour
Cockroach
Cuticular Fatty Acids
Entomopathogenic Fungus
Haemolymph
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49076
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49076
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)Gutierrez, Alejandra ConcepciónMachado, J. A. R.Hubner Campos, R.Pennisi, M. A.Rodrigues, J.Lopez Lastra, Claudia CristinaGarcia, Juan JoseFernandes, É. K. K.Luz, C.BehaviourCockroachCuticular Fatty AcidsEntomopathogenic FungusHaemolymphhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: To study the marked resistance of Periplaneta americana to entomopathogenic Metarhizium anisopliae. Methods and Results: The low susceptibility of 4th instar nymphs applied topically with conidia seemed to be related to an active removal of conidia by the cockroach and to a disabled or retarded germination and subsequent development of conidia on the cuticle (up to 80% germination in the next 7 days after application). Inhibitions or delays of germination were related to the composition of the epicuticular fatty acids (30·1% w/w oleic, 28·3% w/w linoleic, 24·5% w/w palmitic and 11·7% w/w stearic acid) reported here. Propagules invading the nymphs through the cuticle took at least 3 days to reach the haemocoel, and no propagules were found after day 8 post-treatment. Strain IP 46 infected >50% of nymphs treated with doses ≥2 × 104 hyphal bodies (HB) nymph−1 and reduced the survival of nymphs ≤50%. Most nymphs (>70%) survived after injection of 6 × 103 and 2 × 103 HB nymph−1. Conclusions: Findings emphasize a distinct resistance of nymphs of the American cockroach to infections by M. anisopliae. Significance and Impact of the Study: Our findings provide support for the development of biological control of this synanthropic cockroach pest.Fil: Gutierrez, Alejandra Concepción. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Machado, J. A. R.. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Hubner Campos, R.. Universidade Federal de Goiás; BrasilFil: Pennisi, M. A.. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Rodrigues, J.. Universidade Federal de Goiás; BrasilFil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Garcia, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Fernandes, É. K. K.. Universidade Federal de Goiás; BrasilFil: Luz, C.. Universidade Federal de Goiás; BrasilWiley Blackwell Publishing, Inc2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49076Gutierrez, Alejandra Concepción; Machado, J. A. R.; Hubner Campos, R.; Pennisi, M. A.; Rodrigues, J.; et al.; New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales); Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 121; 5; 11-2016; 1373-13831364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jam.13254info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13254info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:42Zoai:ri.conicet.gov.ar:11336/49076instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:43.257CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
title New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
spellingShingle New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
Gutierrez, Alejandra Concepción
Behaviour
Cockroach
Cuticular Fatty Acids
Entomopathogenic Fungus
Haemolymph
title_short New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
title_full New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
title_fullStr New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
title_full_unstemmed New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
title_sort New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales)
dc.creator.none.fl_str_mv Gutierrez, Alejandra Concepción
Machado, J. A. R.
Hubner Campos, R.
Pennisi, M. A.
Rodrigues, J.
Lopez Lastra, Claudia Cristina
Garcia, Juan Jose
Fernandes, É. K. K.
Luz, C.
author Gutierrez, Alejandra Concepción
author_facet Gutierrez, Alejandra Concepción
Machado, J. A. R.
Hubner Campos, R.
Pennisi, M. A.
Rodrigues, J.
Lopez Lastra, Claudia Cristina
Garcia, Juan Jose
Fernandes, É. K. K.
Luz, C.
author_role author
author2 Machado, J. A. R.
Hubner Campos, R.
Pennisi, M. A.
Rodrigues, J.
Lopez Lastra, Claudia Cristina
Garcia, Juan Jose
Fernandes, É. K. K.
Luz, C.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Behaviour
Cockroach
Cuticular Fatty Acids
Entomopathogenic Fungus
Haemolymph
topic Behaviour
Cockroach
Cuticular Fatty Acids
Entomopathogenic Fungus
Haemolymph
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: To study the marked resistance of Periplaneta americana to entomopathogenic Metarhizium anisopliae. Methods and Results: The low susceptibility of 4th instar nymphs applied topically with conidia seemed to be related to an active removal of conidia by the cockroach and to a disabled or retarded germination and subsequent development of conidia on the cuticle (up to 80% germination in the next 7 days after application). Inhibitions or delays of germination were related to the composition of the epicuticular fatty acids (30·1% w/w oleic, 28·3% w/w linoleic, 24·5% w/w palmitic and 11·7% w/w stearic acid) reported here. Propagules invading the nymphs through the cuticle took at least 3 days to reach the haemocoel, and no propagules were found after day 8 post-treatment. Strain IP 46 infected >50% of nymphs treated with doses ≥2 × 104 hyphal bodies (HB) nymph−1 and reduced the survival of nymphs ≤50%. Most nymphs (>70%) survived after injection of 6 × 103 and 2 × 103 HB nymph−1. Conclusions: Findings emphasize a distinct resistance of nymphs of the American cockroach to infections by M. anisopliae. Significance and Impact of the Study: Our findings provide support for the development of biological control of this synanthropic cockroach pest.
Fil: Gutierrez, Alejandra Concepción. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Machado, J. A. R.. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Hubner Campos, R.. Universidade Federal de Goiás; Brasil
Fil: Pennisi, M. A.. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Rodrigues, J.. Universidade Federal de Goiás; Brasil
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Garcia, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Fernandes, É. K. K.. Universidade Federal de Goiás; Brasil
Fil: Luz, C.. Universidade Federal de Goiás; Brasil
description Aims: To study the marked resistance of Periplaneta americana to entomopathogenic Metarhizium anisopliae. Methods and Results: The low susceptibility of 4th instar nymphs applied topically with conidia seemed to be related to an active removal of conidia by the cockroach and to a disabled or retarded germination and subsequent development of conidia on the cuticle (up to 80% germination in the next 7 days after application). Inhibitions or delays of germination were related to the composition of the epicuticular fatty acids (30·1% w/w oleic, 28·3% w/w linoleic, 24·5% w/w palmitic and 11·7% w/w stearic acid) reported here. Propagules invading the nymphs through the cuticle took at least 3 days to reach the haemocoel, and no propagules were found after day 8 post-treatment. Strain IP 46 infected >50% of nymphs treated with doses ≥2 × 104 hyphal bodies (HB) nymph−1 and reduced the survival of nymphs ≤50%. Most nymphs (>70%) survived after injection of 6 × 103 and 2 × 103 HB nymph−1. Conclusions: Findings emphasize a distinct resistance of nymphs of the American cockroach to infections by M. anisopliae. Significance and Impact of the Study: Our findings provide support for the development of biological control of this synanthropic cockroach pest.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49076
Gutierrez, Alejandra Concepción; Machado, J. A. R.; Hubner Campos, R.; Pennisi, M. A.; Rodrigues, J.; et al.; New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales); Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 121; 5; 11-2016; 1373-1383
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49076
identifier_str_mv Gutierrez, Alejandra Concepción; Machado, J. A. R.; Hubner Campos, R.; Pennisi, M. A.; Rodrigues, J.; et al.; New insights into the infection of the American cockroach Periplaneta americana nymphs with Metarhizium anisopliae s.l. (Ascomycota: Hypocreales); Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 121; 5; 11-2016; 1373-1383
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13254
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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