A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans

Autores
Caimi, Karina Cynthia; Varni, Vanina Delia; Meléndez, Yamil Nazareno; Koval, Ariel; Brihuega, Bibiana; Ruybal, Paula
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is an emerging infectious disease that has been identified as both a human and animal health problem worldwide. Regular outbreaks associated with specific risk factors have been reported in Argentina. However, there are no available data concerning the genetic population level for this pathogen. Therefore, the aim of this work was to describe the genetic diversity of Leptospira interrogans through the application of two molecular typing strategies: variable number of tandem repeats (VNTR) and multilocus sequence typing (MLST). For this purpose, seven reference strains and 18 non-epidemiologically related isolates from diverse hosts and Argentinean regions were analysed. Among them, nine genotypes and seven sequence types (STs), including three unreported STs, were described using VNTR and MLST, respectively. eBURST analysis demonstrated that ST37 was the most frequent and founder genotype of a clonal complex (CCs) containing STN1 and STN3, suggesting the importance of studying the serovars belonging to this CC in Argentina. The data from maximum parsimony analysis, which combined both techniques, achieved intra-serovar discrimination, surmounted microscopic agglutination test discrepancies and increased the discriminatory power of each technique applied separately. This study is the first to combine both strategies for L. interrogans typing to generate a more comprehensive molecular genotyping of isolates from Argentina in a global context.
Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Varni, Vanina Delia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Meléndez, Yamil Nazareno. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Koval, Ariel. Biogénesis Bagó; Argentina
Fil: Brihuega, Bibiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
MULTILOCUS SEQUENCE TYPING
VARIABLE NUMBER TANDEM REPEATS
LEPTOSPIRA INTERROGANS
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196596
Acceder
id CONICETDig_d58ffe9db5ada354ead7fe6d35c1582b
oai_identifier_str oai:ri.conicet.gov.ar:11336/196596
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogansCaimi, Karina CynthiaVarni, Vanina DeliaMeléndez, Yamil NazarenoKoval, ArielBrihuega, BibianaRuybal, PaulaMULTILOCUS SEQUENCE TYPINGVARIABLE NUMBER TANDEM REPEATSLEPTOSPIRA INTERROGANSARGENTINAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Leptospirosis is an emerging infectious disease that has been identified as both a human and animal health problem worldwide. Regular outbreaks associated with specific risk factors have been reported in Argentina. However, there are no available data concerning the genetic population level for this pathogen. Therefore, the aim of this work was to describe the genetic diversity of Leptospira interrogans through the application of two molecular typing strategies: variable number of tandem repeats (VNTR) and multilocus sequence typing (MLST). For this purpose, seven reference strains and 18 non-epidemiologically related isolates from diverse hosts and Argentinean regions were analysed. Among them, nine genotypes and seven sequence types (STs), including three unreported STs, were described using VNTR and MLST, respectively. eBURST analysis demonstrated that ST37 was the most frequent and founder genotype of a clonal complex (CCs) containing STN1 and STN3, suggesting the importance of studying the serovars belonging to this CC in Argentina. The data from maximum parsimony analysis, which combined both techniques, achieved intra-serovar discrimination, surmounted microscopic agglutination test discrepancies and increased the discriminatory power of each technique applied separately. This study is the first to combine both strategies for L. interrogans typing to generate a more comprehensive molecular genotyping of isolates from Argentina in a global context.Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Varni, Vanina Delia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Meléndez, Yamil Nazareno. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Koval, Ariel. Biogénesis Bagó; ArgentinaFil: Brihuega, Bibiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFundação Oswaldo Cruz2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196596Caimi, Karina Cynthia; Varni, Vanina Delia; Meléndez, Yamil Nazareno; Koval, Ariel; Brihuega, Bibiana; et al.; A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 107; 5; 6-2012; 644-6510074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/mioc/a/jPKH4GVQQ6qzHTjCZWnCxLJ/?lang=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:52Zoai:ri.conicet.gov.ar:11336/196596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:53.247CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
title A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
spellingShingle A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
Caimi, Karina Cynthia
MULTILOCUS SEQUENCE TYPING
VARIABLE NUMBER TANDEM REPEATS
LEPTOSPIRA INTERROGANS
ARGENTINA
title_short A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
title_full A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
title_fullStr A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
title_full_unstemmed A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
title_sort A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans
dc.creator.none.fl_str_mv Caimi, Karina Cynthia
Varni, Vanina Delia
Meléndez, Yamil Nazareno
Koval, Ariel
Brihuega, Bibiana
Ruybal, Paula
author Caimi, Karina Cynthia
author_facet Caimi, Karina Cynthia
Varni, Vanina Delia
Meléndez, Yamil Nazareno
Koval, Ariel
Brihuega, Bibiana
Ruybal, Paula
author_role author
author2 Varni, Vanina Delia
Meléndez, Yamil Nazareno
Koval, Ariel
Brihuega, Bibiana
Ruybal, Paula
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MULTILOCUS SEQUENCE TYPING
VARIABLE NUMBER TANDEM REPEATS
LEPTOSPIRA INTERROGANS
ARGENTINA
topic MULTILOCUS SEQUENCE TYPING
VARIABLE NUMBER TANDEM REPEATS
LEPTOSPIRA INTERROGANS
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Leptospirosis is an emerging infectious disease that has been identified as both a human and animal health problem worldwide. Regular outbreaks associated with specific risk factors have been reported in Argentina. However, there are no available data concerning the genetic population level for this pathogen. Therefore, the aim of this work was to describe the genetic diversity of Leptospira interrogans through the application of two molecular typing strategies: variable number of tandem repeats (VNTR) and multilocus sequence typing (MLST). For this purpose, seven reference strains and 18 non-epidemiologically related isolates from diverse hosts and Argentinean regions were analysed. Among them, nine genotypes and seven sequence types (STs), including three unreported STs, were described using VNTR and MLST, respectively. eBURST analysis demonstrated that ST37 was the most frequent and founder genotype of a clonal complex (CCs) containing STN1 and STN3, suggesting the importance of studying the serovars belonging to this CC in Argentina. The data from maximum parsimony analysis, which combined both techniques, achieved intra-serovar discrimination, surmounted microscopic agglutination test discrepancies and increased the discriminatory power of each technique applied separately. This study is the first to combine both strategies for L. interrogans typing to generate a more comprehensive molecular genotyping of isolates from Argentina in a global context.
Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Varni, Vanina Delia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Meléndez, Yamil Nazareno. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Koval, Ariel. Biogénesis Bagó; Argentina
Fil: Brihuega, Bibiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Leptospirosis is an emerging infectious disease that has been identified as both a human and animal health problem worldwide. Regular outbreaks associated with specific risk factors have been reported in Argentina. However, there are no available data concerning the genetic population level for this pathogen. Therefore, the aim of this work was to describe the genetic diversity of Leptospira interrogans through the application of two molecular typing strategies: variable number of tandem repeats (VNTR) and multilocus sequence typing (MLST). For this purpose, seven reference strains and 18 non-epidemiologically related isolates from diverse hosts and Argentinean regions were analysed. Among them, nine genotypes and seven sequence types (STs), including three unreported STs, were described using VNTR and MLST, respectively. eBURST analysis demonstrated that ST37 was the most frequent and founder genotype of a clonal complex (CCs) containing STN1 and STN3, suggesting the importance of studying the serovars belonging to this CC in Argentina. The data from maximum parsimony analysis, which combined both techniques, achieved intra-serovar discrimination, surmounted microscopic agglutination test discrepancies and increased the discriminatory power of each technique applied separately. This study is the first to combine both strategies for L. interrogans typing to generate a more comprehensive molecular genotyping of isolates from Argentina in a global context.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196596
Caimi, Karina Cynthia; Varni, Vanina Delia; Meléndez, Yamil Nazareno; Koval, Ariel; Brihuega, Bibiana; et al.; A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 107; 5; 6-2012; 644-651
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196596
identifier_str_mv Caimi, Karina Cynthia; Varni, Vanina Delia; Meléndez, Yamil Nazareno; Koval, Ariel; Brihuega, Bibiana; et al.; A combined approach of VNTR and MLST analysis: Improvement of molecular typing of argentinean isolates of Leptospira interrogans; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 107; 5; 6-2012; 644-651
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/mioc/a/jPKH4GVQQ6qzHTjCZWnCxLJ/?lang=en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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