New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides

Autores
Chatgilialoglu, Chryssostomos; Krokidis, Marios G.; Masi, Annalisa; Barata Vallejo, Sebastian; Ferreri, Carla; Terzidis, Michael A.; Szreder, Tomasz; Bobrowski, Krzysztof
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides and ct-DNA in the range of 1 µs to 1 ms by pulse radiolysis using an Intensified Charged Coupled Device (ICCD) camera. Moreover, we applied our published analytical protocol based on an LC-MS/MS system with isotopomeric internal standards to enable accurate and precise measurements of purine lesion formation. In particular, the simultaneous measurement of the four purine 50,8-cyclo-20-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-20-deoxypurine (8-oxo-Pu) was obtained upon reaction of genetic material with HO• radicals generated either by γ-radiolysis or Fenton-type reactions. Our results contributed to the debate in the literature regarding absolute level of lesions, method of HO• radical generation, 50R/50S diastereomeric ratio in cPu, and relative abundance between cPu and 8-oxo-Pu.
Fil: Chatgilialoglu, Chryssostomos. Istituto Per la Sintesi Organica E la Fotoreattivita, Bologna; Italia
Fil: Krokidis, Marios G.. Consiglio Nazionale delle Ricerche; Italia
Fil: Masi, Annalisa. Consiglio Nazionale delle Ricerche; Italia
Fil: Barata Vallejo, Sebastian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; Italia
Fil: Terzidis, Michael A.. Consiglio Nazionale delle Ricerche; Italia
Fil: Szreder, Tomasz. Institute of Nuclear Chemistry and Technology; Polonia
Fil: Bobrowski, Krzysztof. Institute of Nuclear Chemistry and Technology; Polonia
Materia
50,8-CYCLOPURINES
8-OXO-DG
DNA DAMAGE
FENTON REACTION
FREE RADICALS
GAMMA RADIOLYSIS
OLIGONUCLEOTIDES
PULSE RADIOLYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156176
Acceder
id CONICETDig_d52ebe45f5a1f4e63aae4101520e92ff
oai_identifier_str oai:ri.conicet.gov.ar:11336/156176
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotidesChatgilialoglu, ChryssostomosKrokidis, Marios G.Masi, AnnalisaBarata Vallejo, SebastianFerreri, CarlaTerzidis, Michael A.Szreder, TomaszBobrowski, Krzysztof50,8-CYCLOPURINES8-OXO-DGDNA DAMAGEFENTON REACTIONFREE RADICALSGAMMA RADIOLYSISOLIGONUCLEOTIDESPULSE RADIOLYSIShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides and ct-DNA in the range of 1 µs to 1 ms by pulse radiolysis using an Intensified Charged Coupled Device (ICCD) camera. Moreover, we applied our published analytical protocol based on an LC-MS/MS system with isotopomeric internal standards to enable accurate and precise measurements of purine lesion formation. In particular, the simultaneous measurement of the four purine 50,8-cyclo-20-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-20-deoxypurine (8-oxo-Pu) was obtained upon reaction of genetic material with HO• radicals generated either by γ-radiolysis or Fenton-type reactions. Our results contributed to the debate in the literature regarding absolute level of lesions, method of HO• radical generation, 50R/50S diastereomeric ratio in cPu, and relative abundance between cPu and 8-oxo-Pu.Fil: Chatgilialoglu, Chryssostomos. Istituto Per la Sintesi Organica E la Fotoreattivita, Bologna; ItaliaFil: Krokidis, Marios G.. Consiglio Nazionale delle Ricerche; ItaliaFil: Masi, Annalisa. Consiglio Nazionale delle Ricerche; ItaliaFil: Barata Vallejo, Sebastian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; ItaliaFil: Terzidis, Michael A.. Consiglio Nazionale delle Ricerche; ItaliaFil: Szreder, Tomasz. Institute of Nuclear Chemistry and Technology; PoloniaFil: Bobrowski, Krzysztof. Institute of Nuclear Chemistry and Technology; PoloniaMolecular Diversity Preservation International2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156176Chatgilialoglu, Chryssostomos; Krokidis, Marios G.; Masi, Annalisa; Barata Vallejo, Sebastian; Ferreri, Carla; et al.; New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides; Molecular Diversity Preservation International; Molecules; 24; 21; 10-2019; 1-201420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/24/21/3860info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules24213860info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:22Zoai:ri.conicet.gov.ar:11336/156176instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:22.749CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
title New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
spellingShingle New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
Chatgilialoglu, Chryssostomos
50,8-CYCLOPURINES
8-OXO-DG
DNA DAMAGE
FENTON REACTION
FREE RADICALS
GAMMA RADIOLYSIS
OLIGONUCLEOTIDES
PULSE RADIOLYSIS
title_short New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
title_full New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
title_fullStr New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
title_full_unstemmed New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
title_sort New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
dc.creator.none.fl_str_mv Chatgilialoglu, Chryssostomos
Krokidis, Marios G.
Masi, Annalisa
Barata Vallejo, Sebastian
Ferreri, Carla
Terzidis, Michael A.
Szreder, Tomasz
Bobrowski, Krzysztof
author Chatgilialoglu, Chryssostomos
author_facet Chatgilialoglu, Chryssostomos
Krokidis, Marios G.
Masi, Annalisa
Barata Vallejo, Sebastian
Ferreri, Carla
Terzidis, Michael A.
Szreder, Tomasz
Bobrowski, Krzysztof
author_role author
author2 Krokidis, Marios G.
Masi, Annalisa
Barata Vallejo, Sebastian
Ferreri, Carla
Terzidis, Michael A.
Szreder, Tomasz
Bobrowski, Krzysztof
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 50,8-CYCLOPURINES
8-OXO-DG
DNA DAMAGE
FENTON REACTION
FREE RADICALS
GAMMA RADIOLYSIS
OLIGONUCLEOTIDES
PULSE RADIOLYSIS
topic 50,8-CYCLOPURINES
8-OXO-DG
DNA DAMAGE
FENTON REACTION
FREE RADICALS
GAMMA RADIOLYSIS
OLIGONUCLEOTIDES
PULSE RADIOLYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides and ct-DNA in the range of 1 µs to 1 ms by pulse radiolysis using an Intensified Charged Coupled Device (ICCD) camera. Moreover, we applied our published analytical protocol based on an LC-MS/MS system with isotopomeric internal standards to enable accurate and precise measurements of purine lesion formation. In particular, the simultaneous measurement of the four purine 50,8-cyclo-20-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-20-deoxypurine (8-oxo-Pu) was obtained upon reaction of genetic material with HO• radicals generated either by γ-radiolysis or Fenton-type reactions. Our results contributed to the debate in the literature regarding absolute level of lesions, method of HO• radical generation, 50R/50S diastereomeric ratio in cPu, and relative abundance between cPu and 8-oxo-Pu.
Fil: Chatgilialoglu, Chryssostomos. Istituto Per la Sintesi Organica E la Fotoreattivita, Bologna; Italia
Fil: Krokidis, Marios G.. Consiglio Nazionale delle Ricerche; Italia
Fil: Masi, Annalisa. Consiglio Nazionale delle Ricerche; Italia
Fil: Barata Vallejo, Sebastian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; Italia
Fil: Terzidis, Michael A.. Consiglio Nazionale delle Ricerche; Italia
Fil: Szreder, Tomasz. Institute of Nuclear Chemistry and Technology; Polonia
Fil: Bobrowski, Krzysztof. Institute of Nuclear Chemistry and Technology; Polonia
description The reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides and ct-DNA in the range of 1 µs to 1 ms by pulse radiolysis using an Intensified Charged Coupled Device (ICCD) camera. Moreover, we applied our published analytical protocol based on an LC-MS/MS system with isotopomeric internal standards to enable accurate and precise measurements of purine lesion formation. In particular, the simultaneous measurement of the four purine 50,8-cyclo-20-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-20-deoxypurine (8-oxo-Pu) was obtained upon reaction of genetic material with HO• radicals generated either by γ-radiolysis or Fenton-type reactions. Our results contributed to the debate in the literature regarding absolute level of lesions, method of HO• radical generation, 50R/50S diastereomeric ratio in cPu, and relative abundance between cPu and 8-oxo-Pu.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156176
Chatgilialoglu, Chryssostomos; Krokidis, Marios G.; Masi, Annalisa; Barata Vallejo, Sebastian; Ferreri, Carla; et al.; New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides; Molecular Diversity Preservation International; Molecules; 24; 21; 10-2019; 1-20
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156176
identifier_str_mv Chatgilialoglu, Chryssostomos; Krokidis, Marios G.; Masi, Annalisa; Barata Vallejo, Sebastian; Ferreri, Carla; et al.; New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides; Molecular Diversity Preservation International; Molecules; 24; 21; 10-2019; 1-20
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/24/21/3860
info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules24213860
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613448391983104
score 13.069144

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