Effect of bile on the β-galactosidase activity of dairy propionibacteria

Autores
Zarate, Gabriela del Valle; Gonzalez, Silvia Nelina; Perez Chaia, Adriana Beatriz; Oliver, Guillermo
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study, the effect of bile on the growth, cell permeabilization and β-galactosidase activity of dairy propionibacteria was determined. Two groups of strains with different behavior in media with oxgall (dehydrated fresh bile) could be observed, and were named bile-tolerant and non bile-tolerant groups. The β-galactosidase activity of bile-tolerant strains increased during the growth in media containing 0.15 and 0.30% oxgall. The incubation of cell suspensions in phosphate buffer with oxgall increased the β-galactosidase activity of all the studied strains. They were permeabilized by bile, permitting more substrate to enter the cells to be hydrolyzed by β-galactosidase. A higher specific activity was observed in cell-free extracts obtained from cultures in media with oxgall, indicating increased enzyme synthesis in the presence of bile. The enzyme activity was also increased by sodium taurocholate and sodium chloride. These results suggest that bile-tolerant strains of propionibacteria can provide meaningful β-galactosidase activity by permeabilization of the cells and increased enzyme synthesis during growth in the gut. The non bile-tolerant strains did not grow in media with bile. They were permeabilized, but their β-galactosidase was inactivated when they were exposed for a long time to bile in the growth media. Thus, their contribution to the β-galactosidase activity in the gut does not appear to be significant.
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Perez Chaia, Adriana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Oliver, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Bile Tolerance
Propionibacteria
Β-Galactosidase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78730
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78730
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of bile on the β-galactosidase activity of dairy propionibacteriaEffet de la bile sur l'activité β-galactosidase de bactéries propioniques laitièresZarate, Gabriela del ValleGonzalez, Silvia NelinaPerez Chaia, Adriana BeatrizOliver, GuillermoBile TolerancePropionibacteriaΒ-Galactosidasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study, the effect of bile on the growth, cell permeabilization and β-galactosidase activity of dairy propionibacteria was determined. Two groups of strains with different behavior in media with oxgall (dehydrated fresh bile) could be observed, and were named bile-tolerant and non bile-tolerant groups. The β-galactosidase activity of bile-tolerant strains increased during the growth in media containing 0.15 and 0.30% oxgall. The incubation of cell suspensions in phosphate buffer with oxgall increased the β-galactosidase activity of all the studied strains. They were permeabilized by bile, permitting more substrate to enter the cells to be hydrolyzed by β-galactosidase. A higher specific activity was observed in cell-free extracts obtained from cultures in media with oxgall, indicating increased enzyme synthesis in the presence of bile. The enzyme activity was also increased by sodium taurocholate and sodium chloride. These results suggest that bile-tolerant strains of propionibacteria can provide meaningful β-galactosidase activity by permeabilization of the cells and increased enzyme synthesis during growth in the gut. The non bile-tolerant strains did not grow in media with bile. They were permeabilized, but their β-galactosidase was inactivated when they were exposed for a long time to bile in the growth media. Thus, their contribution to the β-galactosidase activity in the gut does not appear to be significant.Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Perez Chaia, Adriana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Oliver, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaEDP Sciences2000-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78730Zarate, Gabriela del Valle; Gonzalez, Silvia Nelina; Perez Chaia, Adriana Beatriz; Oliver, Guillermo; Effect of bile on the β-galactosidase activity of dairy propionibacteria; EDP Sciences; Lait; 80; 2; 4-2000; 267-2760023-73021297-9694CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1051/lait:2000125info:eu-repo/semantics/altIdentifier/url/https://lait.dairy-journal.org/articles/lait/abs/2000/02/L0206/L0206.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:56Zoai:ri.conicet.gov.ar:11336/78730instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:57.093CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of bile on the β-galactosidase activity of dairy propionibacteria
Effet de la bile sur l'activité β-galactosidase de bactéries propioniques laitières
title Effect of bile on the β-galactosidase activity of dairy propionibacteria
spellingShingle Effect of bile on the β-galactosidase activity of dairy propionibacteria
Zarate, Gabriela del Valle
Bile Tolerance
Propionibacteria
Β-Galactosidase
title_short Effect of bile on the β-galactosidase activity of dairy propionibacteria
title_full Effect of bile on the β-galactosidase activity of dairy propionibacteria
title_fullStr Effect of bile on the β-galactosidase activity of dairy propionibacteria
title_full_unstemmed Effect of bile on the β-galactosidase activity of dairy propionibacteria
title_sort Effect of bile on the β-galactosidase activity of dairy propionibacteria
dc.creator.none.fl_str_mv Zarate, Gabriela del Valle
Gonzalez, Silvia Nelina
Perez Chaia, Adriana Beatriz
Oliver, Guillermo
author Zarate, Gabriela del Valle
author_facet Zarate, Gabriela del Valle
Gonzalez, Silvia Nelina
Perez Chaia, Adriana Beatriz
Oliver, Guillermo
author_role author
author2 Gonzalez, Silvia Nelina
Perez Chaia, Adriana Beatriz
Oliver, Guillermo
author2_role author
author
author
dc.subject.none.fl_str_mv Bile Tolerance
Propionibacteria
Β-Galactosidase
topic Bile Tolerance
Propionibacteria
Β-Galactosidase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present study, the effect of bile on the growth, cell permeabilization and β-galactosidase activity of dairy propionibacteria was determined. Two groups of strains with different behavior in media with oxgall (dehydrated fresh bile) could be observed, and were named bile-tolerant and non bile-tolerant groups. The β-galactosidase activity of bile-tolerant strains increased during the growth in media containing 0.15 and 0.30% oxgall. The incubation of cell suspensions in phosphate buffer with oxgall increased the β-galactosidase activity of all the studied strains. They were permeabilized by bile, permitting more substrate to enter the cells to be hydrolyzed by β-galactosidase. A higher specific activity was observed in cell-free extracts obtained from cultures in media with oxgall, indicating increased enzyme synthesis in the presence of bile. The enzyme activity was also increased by sodium taurocholate and sodium chloride. These results suggest that bile-tolerant strains of propionibacteria can provide meaningful β-galactosidase activity by permeabilization of the cells and increased enzyme synthesis during growth in the gut. The non bile-tolerant strains did not grow in media with bile. They were permeabilized, but their β-galactosidase was inactivated when they were exposed for a long time to bile in the growth media. Thus, their contribution to the β-galactosidase activity in the gut does not appear to be significant.
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Perez Chaia, Adriana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Oliver, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description In the present study, the effect of bile on the growth, cell permeabilization and β-galactosidase activity of dairy propionibacteria was determined. Two groups of strains with different behavior in media with oxgall (dehydrated fresh bile) could be observed, and were named bile-tolerant and non bile-tolerant groups. The β-galactosidase activity of bile-tolerant strains increased during the growth in media containing 0.15 and 0.30% oxgall. The incubation of cell suspensions in phosphate buffer with oxgall increased the β-galactosidase activity of all the studied strains. They were permeabilized by bile, permitting more substrate to enter the cells to be hydrolyzed by β-galactosidase. A higher specific activity was observed in cell-free extracts obtained from cultures in media with oxgall, indicating increased enzyme synthesis in the presence of bile. The enzyme activity was also increased by sodium taurocholate and sodium chloride. These results suggest that bile-tolerant strains of propionibacteria can provide meaningful β-galactosidase activity by permeabilization of the cells and increased enzyme synthesis during growth in the gut. The non bile-tolerant strains did not grow in media with bile. They were permeabilized, but their β-galactosidase was inactivated when they were exposed for a long time to bile in the growth media. Thus, their contribution to the β-galactosidase activity in the gut does not appear to be significant.
publishDate 2000
dc.date.none.fl_str_mv 2000-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78730
Zarate, Gabriela del Valle; Gonzalez, Silvia Nelina; Perez Chaia, Adriana Beatriz; Oliver, Guillermo; Effect of bile on the β-galactosidase activity of dairy propionibacteria; EDP Sciences; Lait; 80; 2; 4-2000; 267-276
0023-7302
1297-9694
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78730
identifier_str_mv Zarate, Gabriela del Valle; Gonzalez, Silvia Nelina; Perez Chaia, Adriana Beatriz; Oliver, Guillermo; Effect of bile on the β-galactosidase activity of dairy propionibacteria; EDP Sciences; Lait; 80; 2; 4-2000; 267-276
0023-7302
1297-9694
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1051/lait:2000125
info:eu-repo/semantics/altIdentifier/url/https://lait.dairy-journal.org/articles/lait/abs/2000/02/L0206/L0206.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv EDP Sciences
publisher.none.fl_str_mv EDP Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614366806147072
score 13.070432

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