Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice

Autores
Brugnoni, Lorena Inés; Lozano, Jorge Enrique; Cubitto, María Amelia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we evaluated the effectiveness of sodium hypochlorite and a commercial quaternary ammonium compound against four wild yeast strains in their different growth forms: planktonic, sessile and forming microcolonies. These yeasts had been isolated from apple juice processing plant, and appropriate environmental conditions were employed throughout the work. For planktonic cells, 4 log reduction was obtained using the lowest concentration of sodium hypochlorite (200 ppm) on all tested strains. However, quaternary ammonium chloride was effective only on Zygosaccharomyces sp. in all in-use concentrations tested, and only the stronger in-use concentration (1:30) was effective on Rhodotorula mucilaginosa. Because of the above results, the disinfection on sessile yeast cells was performed only with sodium hypochlorite solutions. In cells attached in batch conditions, the highest concentration of sodium hypochlorite (500 ppm) was necessary to get 4 log reduction. In order to obtain a 3 log reduction on Zygosaccharomyces sp. microcolonies formed in laminar flow conditions, the combined action of 0.5% w/v NaOH and 500 ppm sodium hypochlorite was required. We concluded, thus, that cells forming microcolonies under laminar flow conditions were less susceptible to disinfectants than those suspended or attached in batch conditions. These results also indicated that attachment and colonization of autochthonous yeasts must be properly identified to evaluate the effectiveness of disinfectants used in apple juice processing plants.
Fil: Brugnoni, Lorena Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozano, Jorge Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Cubitto, María Amelia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Materia
Yeast
Biofilm
Cleaning
Disinfectant
Stainless Steel
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55938
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juiceBrugnoni, Lorena InésLozano, Jorge EnriqueCubitto, María AmeliaYeastBiofilmCleaningDisinfectantStainless Steelhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2In this work, we evaluated the effectiveness of sodium hypochlorite and a commercial quaternary ammonium compound against four wild yeast strains in their different growth forms: planktonic, sessile and forming microcolonies. These yeasts had been isolated from apple juice processing plant, and appropriate environmental conditions were employed throughout the work. For planktonic cells, 4 log reduction was obtained using the lowest concentration of sodium hypochlorite (200 ppm) on all tested strains. However, quaternary ammonium chloride was effective only on Zygosaccharomyces sp. in all in-use concentrations tested, and only the stronger in-use concentration (1:30) was effective on Rhodotorula mucilaginosa. Because of the above results, the disinfection on sessile yeast cells was performed only with sodium hypochlorite solutions. In cells attached in batch conditions, the highest concentration of sodium hypochlorite (500 ppm) was necessary to get 4 log reduction. In order to obtain a 3 log reduction on Zygosaccharomyces sp. microcolonies formed in laminar flow conditions, the combined action of 0.5% w/v NaOH and 500 ppm sodium hypochlorite was required. We concluded, thus, that cells forming microcolonies under laminar flow conditions were less susceptible to disinfectants than those suspended or attached in batch conditions. These results also indicated that attachment and colonization of autochthonous yeasts must be properly identified to evaluate the effectiveness of disinfectants used in apple juice processing plants.Fil: Brugnoni, Lorena Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lozano, Jorge Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Cubitto, María Amelia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaWiley Blackwell Publishing, Inc2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55938Brugnoni, Lorena Inés; Lozano, Jorge Enrique; Cubitto, María Amelia; Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice; Wiley Blackwell Publishing, Inc; Journal Of Food Process Engineering; 35; 1; 2-2012; 104-1190145-8876CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1745-4530.2009.00574.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1745-4530.2009.00574.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:44Zoai:ri.conicet.gov.ar:11336/55938instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:44.682CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
title Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
spellingShingle Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
Brugnoni, Lorena Inés
Yeast
Biofilm
Cleaning
Disinfectant
Stainless Steel
title_short Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
title_full Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
title_fullStr Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
title_full_unstemmed Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
title_sort Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice
dc.creator.none.fl_str_mv Brugnoni, Lorena Inés
Lozano, Jorge Enrique
Cubitto, María Amelia
author Brugnoni, Lorena Inés
author_facet Brugnoni, Lorena Inés
Lozano, Jorge Enrique
Cubitto, María Amelia
author_role author
author2 Lozano, Jorge Enrique
Cubitto, María Amelia
author2_role author
author
dc.subject.none.fl_str_mv Yeast
Biofilm
Cleaning
Disinfectant
Stainless Steel
topic Yeast
Biofilm
Cleaning
Disinfectant
Stainless Steel
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this work, we evaluated the effectiveness of sodium hypochlorite and a commercial quaternary ammonium compound against four wild yeast strains in their different growth forms: planktonic, sessile and forming microcolonies. These yeasts had been isolated from apple juice processing plant, and appropriate environmental conditions were employed throughout the work. For planktonic cells, 4 log reduction was obtained using the lowest concentration of sodium hypochlorite (200 ppm) on all tested strains. However, quaternary ammonium chloride was effective only on Zygosaccharomyces sp. in all in-use concentrations tested, and only the stronger in-use concentration (1:30) was effective on Rhodotorula mucilaginosa. Because of the above results, the disinfection on sessile yeast cells was performed only with sodium hypochlorite solutions. In cells attached in batch conditions, the highest concentration of sodium hypochlorite (500 ppm) was necessary to get 4 log reduction. In order to obtain a 3 log reduction on Zygosaccharomyces sp. microcolonies formed in laminar flow conditions, the combined action of 0.5% w/v NaOH and 500 ppm sodium hypochlorite was required. We concluded, thus, that cells forming microcolonies under laminar flow conditions were less susceptible to disinfectants than those suspended or attached in batch conditions. These results also indicated that attachment and colonization of autochthonous yeasts must be properly identified to evaluate the effectiveness of disinfectants used in apple juice processing plants.
Fil: Brugnoni, Lorena Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozano, Jorge Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Cubitto, María Amelia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description In this work, we evaluated the effectiveness of sodium hypochlorite and a commercial quaternary ammonium compound against four wild yeast strains in their different growth forms: planktonic, sessile and forming microcolonies. These yeasts had been isolated from apple juice processing plant, and appropriate environmental conditions were employed throughout the work. For planktonic cells, 4 log reduction was obtained using the lowest concentration of sodium hypochlorite (200 ppm) on all tested strains. However, quaternary ammonium chloride was effective only on Zygosaccharomyces sp. in all in-use concentrations tested, and only the stronger in-use concentration (1:30) was effective on Rhodotorula mucilaginosa. Because of the above results, the disinfection on sessile yeast cells was performed only with sodium hypochlorite solutions. In cells attached in batch conditions, the highest concentration of sodium hypochlorite (500 ppm) was necessary to get 4 log reduction. In order to obtain a 3 log reduction on Zygosaccharomyces sp. microcolonies formed in laminar flow conditions, the combined action of 0.5% w/v NaOH and 500 ppm sodium hypochlorite was required. We concluded, thus, that cells forming microcolonies under laminar flow conditions were less susceptible to disinfectants than those suspended or attached in batch conditions. These results also indicated that attachment and colonization of autochthonous yeasts must be properly identified to evaluate the effectiveness of disinfectants used in apple juice processing plants.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55938
Brugnoni, Lorena Inés; Lozano, Jorge Enrique; Cubitto, María Amelia; Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice; Wiley Blackwell Publishing, Inc; Journal Of Food Process Engineering; 35; 1; 2-2012; 104-119
0145-8876
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55938
identifier_str_mv Brugnoni, Lorena Inés; Lozano, Jorge Enrique; Cubitto, María Amelia; Efficacy of sodium hypochlorite and quaternary ammonium compounds on yeasts isolated from apple juice; Wiley Blackwell Publishing, Inc; Journal Of Food Process Engineering; 35; 1; 2-2012; 104-119
0145-8876
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1745-4530.2009.00574.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1745-4530.2009.00574.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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