Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program

Autores
Brugnoni, Lorena Inés; Cubitto, María Amelia; Lozano, Jorge Enrique
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In food processing lines or in complex equipment such as pumps or valves, microorganisms are exposed to varying hydrodynamic conditions caused by the flow of liquid food, and biofilms are thus grown under a wide distribution of local hydrodynamic strengths. Using an industrially relevant strain of Candida krusei, we demonstrated that biofilms formed on stainless steel for 4 days at Reynolds (Re) numbers ranging from 294,000 to 1.2 x 106 proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in an extracellular matrix and biofilm formation increases when increasing Reynolds number and time. In all growth phases, the morphology of C. krusei biofilm revealed the influence of hydrodynamic drag. Indeed, we study the effect of cleaning and sanitation procedure in the control of turbulent flow-generated biofilm. This procedure involves alkali (NaOH 0.5 %) and sodium hypochlorite (500 ppm). In terms of total biofilm mass, removal decreases with increasing biofilm age. The largest reduction post-treatment (between 57-62%) was observed, to all Reynolds numbers, on 24 h and 48h- old biofilms. Removal was between 39 and 46 % on 72 h-old biofilms and was close to 30 % for all Reynolds numbers on 96 h-old biofilm.
Fil: Brugnoni, Lorena Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Cubitto, María Amelia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Lozano, Jorge Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
TURBULENT FLOW
YEAST
BIOFILMS
STAINLESS STEEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/270837
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection programBrugnoni, Lorena InésCubitto, María AmeliaLozano, Jorge EnriqueTURBULENT FLOWYEASTBIOFILMSSTAINLESS STEELhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2In food processing lines or in complex equipment such as pumps or valves, microorganisms are exposed to varying hydrodynamic conditions caused by the flow of liquid food, and biofilms are thus grown under a wide distribution of local hydrodynamic strengths. Using an industrially relevant strain of Candida krusei, we demonstrated that biofilms formed on stainless steel for 4 days at Reynolds (Re) numbers ranging from 294,000 to 1.2 x 106 proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in an extracellular matrix and biofilm formation increases when increasing Reynolds number and time. In all growth phases, the morphology of C. krusei biofilm revealed the influence of hydrodynamic drag. Indeed, we study the effect of cleaning and sanitation procedure in the control of turbulent flow-generated biofilm. This procedure involves alkali (NaOH 0.5 %) and sodium hypochlorite (500 ppm). In terms of total biofilm mass, removal decreases with increasing biofilm age. The largest reduction post-treatment (between 57-62%) was observed, to all Reynolds numbers, on 24 h and 48h- old biofilms. Removal was between 39 and 46 % on 72 h-old biofilms and was close to 30 % for all Reynolds numbers on 96 h-old biofilm.Fil: Brugnoni, Lorena Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Cubitto, María Amelia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Lozano, Jorge Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaElsevier2012-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270837Brugnoni, Lorena Inés; Cubitto, María Amelia; Lozano, Jorge Enrique; Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program; Elsevier; Journal of Food Engineering; 111; 4; 22-8-2012; 546-5520260-8774CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.journals.elsevier.com/journal-of-food-engineering/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jfoodeng.2012.03.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:27Zoai:ri.conicet.gov.ar:11336/270837instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:28.247CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
title Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
spellingShingle Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
Brugnoni, Lorena Inés
TURBULENT FLOW
YEAST
BIOFILMS
STAINLESS STEEL
title_short Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
title_full Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
title_fullStr Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
title_full_unstemmed Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
title_sort Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program
dc.creator.none.fl_str_mv Brugnoni, Lorena Inés
Cubitto, María Amelia
Lozano, Jorge Enrique
author Brugnoni, Lorena Inés
author_facet Brugnoni, Lorena Inés
Cubitto, María Amelia
Lozano, Jorge Enrique
author_role author
author2 Cubitto, María Amelia
Lozano, Jorge Enrique
author2_role author
author
dc.subject.none.fl_str_mv TURBULENT FLOW
YEAST
BIOFILMS
STAINLESS STEEL
topic TURBULENT FLOW
YEAST
BIOFILMS
STAINLESS STEEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In food processing lines or in complex equipment such as pumps or valves, microorganisms are exposed to varying hydrodynamic conditions caused by the flow of liquid food, and biofilms are thus grown under a wide distribution of local hydrodynamic strengths. Using an industrially relevant strain of Candida krusei, we demonstrated that biofilms formed on stainless steel for 4 days at Reynolds (Re) numbers ranging from 294,000 to 1.2 x 106 proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in an extracellular matrix and biofilm formation increases when increasing Reynolds number and time. In all growth phases, the morphology of C. krusei biofilm revealed the influence of hydrodynamic drag. Indeed, we study the effect of cleaning and sanitation procedure in the control of turbulent flow-generated biofilm. This procedure involves alkali (NaOH 0.5 %) and sodium hypochlorite (500 ppm). In terms of total biofilm mass, removal decreases with increasing biofilm age. The largest reduction post-treatment (between 57-62%) was observed, to all Reynolds numbers, on 24 h and 48h- old biofilms. Removal was between 39 and 46 % on 72 h-old biofilms and was close to 30 % for all Reynolds numbers on 96 h-old biofilm.
Fil: Brugnoni, Lorena Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Cubitto, María Amelia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Lozano, Jorge Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description In food processing lines or in complex equipment such as pumps or valves, microorganisms are exposed to varying hydrodynamic conditions caused by the flow of liquid food, and biofilms are thus grown under a wide distribution of local hydrodynamic strengths. Using an industrially relevant strain of Candida krusei, we demonstrated that biofilms formed on stainless steel for 4 days at Reynolds (Re) numbers ranging from 294,000 to 1.2 x 106 proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in an extracellular matrix and biofilm formation increases when increasing Reynolds number and time. In all growth phases, the morphology of C. krusei biofilm revealed the influence of hydrodynamic drag. Indeed, we study the effect of cleaning and sanitation procedure in the control of turbulent flow-generated biofilm. This procedure involves alkali (NaOH 0.5 %) and sodium hypochlorite (500 ppm). In terms of total biofilm mass, removal decreases with increasing biofilm age. The largest reduction post-treatment (between 57-62%) was observed, to all Reynolds numbers, on 24 h and 48h- old biofilms. Removal was between 39 and 46 % on 72 h-old biofilms and was close to 30 % for all Reynolds numbers on 96 h-old biofilm.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/270837
Brugnoni, Lorena Inés; Cubitto, María Amelia; Lozano, Jorge Enrique; Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program; Elsevier; Journal of Food Engineering; 111; 4; 22-8-2012; 546-552
0260-8774
CONICET Digital
CONICET
url http://hdl.handle.net/11336/270837
identifier_str_mv Brugnoni, Lorena Inés; Cubitto, María Amelia; Lozano, Jorge Enrique; Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program; Elsevier; Journal of Food Engineering; 111; 4; 22-8-2012; 546-552
0260-8774
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.journals.elsevier.com/journal-of-food-engineering/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jfoodeng.2012.03.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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