Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity

Autores
Rodriguez Brizi, Margarita; Marrassini, Carla; Zettler, Gabriela Vanina; Ferraro, Graciela Ester; Anesini, Claudia Alejandra
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tilia species have been used in Asia, Europe and in America to treat anxiety and also for the treatment of colds and in-flammation. The oxygen reactive species (ROS) (hydrogen peroxide (H2O2) and the superoxide anion (O2) are involve in the balance cell proliferation/death in lymphocytes. It was reported the presence of flavonoids in Tilia species which possess antioxidant properties. The aim of this study was to determine comparatively the effect of an aqueous (AE) and ethanol (E) extract from Tilia x viridis, on the proliferation of tumoral and normal concanavalin A stimulated murine lymphocytes in relation to antioxidant activities such as peroxidase (Px), catalase (CAT) and superoxide dismutase (SOD) activities involves in H2O2 modulation. Also a phytochemical pattern of the two extracts in relation to flavonoids content was determined. Both extracts presented antiproliferative action on both type of lymphocytes but E was more selective on the tumoral lymphocytes inhibition (EC50 (μg/ml, Mean ±SEM) (tumoral): 50 ± 4; EC50; (normal lympho-cytes): 323 ± 20); this action was related to a high polyphenols content (150 ± 10 mg/g extract) and high “per se” SOD and low Px activities. In conclusion, the extracts could be a source of antioxidant compounds which contribute to a se-lective antiproliferative action on tumoral cells, acting through the modulation of H2O2 levels
Fil: Rodriguez Brizi, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Marrassini, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Zettler, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Ferraro, Graciela Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Anesini, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Materia
TILIA x VIRIDIS
NORMAL LYMPHOCYTES
TUMORAL LYMPHOCYTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67399
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67399
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant ActivityRodriguez Brizi, MargaritaMarrassini, CarlaZettler, Gabriela VaninaFerraro, Graciela EsterAnesini, Claudia AlejandraTILIA x VIRIDISNORMAL LYMPHOCYTESTUMORAL LYMPHOCYTEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tilia species have been used in Asia, Europe and in America to treat anxiety and also for the treatment of colds and in-flammation. The oxygen reactive species (ROS) (hydrogen peroxide (H2O2) and the superoxide anion (O2) are involve in the balance cell proliferation/death in lymphocytes. It was reported the presence of flavonoids in Tilia species which possess antioxidant properties. The aim of this study was to determine comparatively the effect of an aqueous (AE) and ethanol (E) extract from Tilia x viridis, on the proliferation of tumoral and normal concanavalin A stimulated murine lymphocytes in relation to antioxidant activities such as peroxidase (Px), catalase (CAT) and superoxide dismutase (SOD) activities involves in H2O2 modulation. Also a phytochemical pattern of the two extracts in relation to flavonoids content was determined. Both extracts presented antiproliferative action on both type of lymphocytes but E was more selective on the tumoral lymphocytes inhibition (EC50 (μg/ml, Mean ±SEM) (tumoral): 50 ± 4; EC50; (normal lympho-cytes): 323 ± 20); this action was related to a high polyphenols content (150 ± 10 mg/g extract) and high “per se” SOD and low Px activities. In conclusion, the extracts could be a source of antioxidant compounds which contribute to a se-lective antiproliferative action on tumoral cells, acting through the modulation of H2O2 levelsFil: Rodriguez Brizi, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Marrassini, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Zettler, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Ferraro, Graciela Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Anesini, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaScientific Research2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67399Rodriguez Brizi, Margarita; Marrassini, Carla; Zettler, Gabriela Vanina; Ferraro, Graciela Ester; Anesini, Claudia Alejandra; Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity; Scientific Research; Chinese Medicine; 03; 01; 12-2012; 20-292151-1926CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4236/cm.2012.31005info:eu-repo/semantics/altIdentifier/url/https://file.scirp.org/Html/5-8801095_18051.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:28Zoai:ri.conicet.gov.ar:11336/67399instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:29.128CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
title Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
spellingShingle Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
Rodriguez Brizi, Margarita
TILIA x VIRIDIS
NORMAL LYMPHOCYTES
TUMORAL LYMPHOCYTES
title_short Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
title_full Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
title_fullStr Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
title_full_unstemmed Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
title_sort Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity
dc.creator.none.fl_str_mv Rodriguez Brizi, Margarita
Marrassini, Carla
Zettler, Gabriela Vanina
Ferraro, Graciela Ester
Anesini, Claudia Alejandra
author Rodriguez Brizi, Margarita
author_facet Rodriguez Brizi, Margarita
Marrassini, Carla
Zettler, Gabriela Vanina
Ferraro, Graciela Ester
Anesini, Claudia Alejandra
author_role author
author2 Marrassini, Carla
Zettler, Gabriela Vanina
Ferraro, Graciela Ester
Anesini, Claudia Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv TILIA x VIRIDIS
NORMAL LYMPHOCYTES
TUMORAL LYMPHOCYTES
topic TILIA x VIRIDIS
NORMAL LYMPHOCYTES
TUMORAL LYMPHOCYTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tilia species have been used in Asia, Europe and in America to treat anxiety and also for the treatment of colds and in-flammation. The oxygen reactive species (ROS) (hydrogen peroxide (H2O2) and the superoxide anion (O2) are involve in the balance cell proliferation/death in lymphocytes. It was reported the presence of flavonoids in Tilia species which possess antioxidant properties. The aim of this study was to determine comparatively the effect of an aqueous (AE) and ethanol (E) extract from Tilia x viridis, on the proliferation of tumoral and normal concanavalin A stimulated murine lymphocytes in relation to antioxidant activities such as peroxidase (Px), catalase (CAT) and superoxide dismutase (SOD) activities involves in H2O2 modulation. Also a phytochemical pattern of the two extracts in relation to flavonoids content was determined. Both extracts presented antiproliferative action on both type of lymphocytes but E was more selective on the tumoral lymphocytes inhibition (EC50 (μg/ml, Mean ±SEM) (tumoral): 50 ± 4; EC50; (normal lympho-cytes): 323 ± 20); this action was related to a high polyphenols content (150 ± 10 mg/g extract) and high “per se” SOD and low Px activities. In conclusion, the extracts could be a source of antioxidant compounds which contribute to a se-lective antiproliferative action on tumoral cells, acting through the modulation of H2O2 levels
Fil: Rodriguez Brizi, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Marrassini, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Zettler, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Ferraro, Graciela Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Anesini, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
description Tilia species have been used in Asia, Europe and in America to treat anxiety and also for the treatment of colds and in-flammation. The oxygen reactive species (ROS) (hydrogen peroxide (H2O2) and the superoxide anion (O2) are involve in the balance cell proliferation/death in lymphocytes. It was reported the presence of flavonoids in Tilia species which possess antioxidant properties. The aim of this study was to determine comparatively the effect of an aqueous (AE) and ethanol (E) extract from Tilia x viridis, on the proliferation of tumoral and normal concanavalin A stimulated murine lymphocytes in relation to antioxidant activities such as peroxidase (Px), catalase (CAT) and superoxide dismutase (SOD) activities involves in H2O2 modulation. Also a phytochemical pattern of the two extracts in relation to flavonoids content was determined. Both extracts presented antiproliferative action on both type of lymphocytes but E was more selective on the tumoral lymphocytes inhibition (EC50 (μg/ml, Mean ±SEM) (tumoral): 50 ± 4; EC50; (normal lympho-cytes): 323 ± 20); this action was related to a high polyphenols content (150 ± 10 mg/g extract) and high “per se” SOD and low Px activities. In conclusion, the extracts could be a source of antioxidant compounds which contribute to a se-lective antiproliferative action on tumoral cells, acting through the modulation of H2O2 levels
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67399
Rodriguez Brizi, Margarita; Marrassini, Carla; Zettler, Gabriela Vanina; Ferraro, Graciela Ester; Anesini, Claudia Alejandra; Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity; Scientific Research; Chinese Medicine; 03; 01; 12-2012; 20-29
2151-1926
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67399
identifier_str_mv Rodriguez Brizi, Margarita; Marrassini, Carla; Zettler, Gabriela Vanina; Ferraro, Graciela Ester; Anesini, Claudia Alejandra; Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity; Scientific Research; Chinese Medicine; 03; 01; 12-2012; 20-29
2151-1926
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/cm.2012.31005
info:eu-repo/semantics/altIdentifier/url/https://file.scirp.org/Html/5-8801095_18051.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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