Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study

Autores
Massa, Alicia N.; Bressano, Marina; Soave, Juan H.; Buteler, Mario I.; Seijo, José Guillermo; Sobolev, Victor S.; Orner, Valerie A.; Oddino, Claudio Marcelo; Soave, Sara J.; Faustinelli, Paola Carmen; de Blas, Francisco Javier; Lamb, Marshall C.; Arias, Renee S.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical (P < 0.01, bootstrap t-test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers.
Fil: Massa, Alicia N.. National Peanut Research Laboratory; Estados Unidos
Fil: Bressano, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Soave, Juan H.. Criadero El Carmen; Argentina
Fil: Buteler, Mario I.. Criadero El Carmen; Argentina
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sobolev, Victor S.. National Peanut Research Laboratory; Estados Unidos
Fil: Orner, Valerie A.. National Peanut Research Laboratory; Estados Unidos
Fil: Oddino, Claudio Marcelo. Criadero El Carmen; Argentina
Fil: Soave, Sara J.. Criadero El Carmen; Argentina
Fil: Faustinelli, Paola Carmen. National Peanut Research Laboratory; Estados Unidos
Fil: de Blas, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Lamb, Marshall C.. National Peanut Research Laboratory; Estados Unidos
Fil: Arias, Renee S.. National Peanut Research Laboratory; Estados Unidos
Materia
ARACHIS HYPOGAEA
GENETIC INTROGRESSION
PEANUT
PEANUT SMUT
RHAMP ASSAY
SNP GENOTYPING
THECAPHORA FREZII
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/136026
Acceder
id CONICETDig_d29592b333cc79922b53814813703f45
oai_identifier_str oai:ri.conicet.gov.ar:11336/136026
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case studyMassa, Alicia N.Bressano, MarinaSoave, Juan H.Buteler, Mario I.Seijo, José GuillermoSobolev, Victor S.Orner, Valerie A.Oddino, Claudio MarceloSoave, Sara J.Faustinelli, Paola Carmende Blas, Francisco JavierLamb, Marshall C.Arias, Renee S.ARACHIS HYPOGAEAGENETIC INTROGRESSIONPEANUTPEANUT SMUTRHAMP ASSAYSNP GENOTYPINGTHECAPHORA FREZIIhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical (P < 0.01, bootstrap t-test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers.Fil: Massa, Alicia N.. National Peanut Research Laboratory; Estados UnidosFil: Bressano, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Soave, Juan H.. Criadero El Carmen; ArgentinaFil: Buteler, Mario I.. Criadero El Carmen; ArgentinaFil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Sobolev, Victor S.. National Peanut Research Laboratory; Estados UnidosFil: Orner, Valerie A.. National Peanut Research Laboratory; Estados UnidosFil: Oddino, Claudio Marcelo. Criadero El Carmen; ArgentinaFil: Soave, Sara J.. Criadero El Carmen; ArgentinaFil: Faustinelli, Paola Carmen. National Peanut Research Laboratory; Estados UnidosFil: de Blas, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Lamb, Marshall C.. National Peanut Research Laboratory; Estados UnidosFil: Arias, Renee S.. National Peanut Research Laboratory; Estados UnidosPeerJ Inc.2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/136026Massa, Alicia N.; Bressano, Marina; Soave, Juan H.; Buteler, Mario I.; Seijo, José Guillermo; et al.; Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study; PeerJ Inc.; PeerJ; 9; 1-2021; 1-182167-8359CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/10581/#info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.10581info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:51Zoai:ri.conicet.gov.ar:11336/136026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:52.186CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
title Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
spellingShingle Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
Massa, Alicia N.
ARACHIS HYPOGAEA
GENETIC INTROGRESSION
PEANUT
PEANUT SMUT
RHAMP ASSAY
SNP GENOTYPING
THECAPHORA FREZII
title_short Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
title_full Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
title_fullStr Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
title_full_unstemmed Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
title_sort Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study
dc.creator.none.fl_str_mv Massa, Alicia N.
Bressano, Marina
Soave, Juan H.
Buteler, Mario I.
Seijo, José Guillermo
Sobolev, Victor S.
Orner, Valerie A.
Oddino, Claudio Marcelo
Soave, Sara J.
Faustinelli, Paola Carmen
de Blas, Francisco Javier
Lamb, Marshall C.
Arias, Renee S.
author Massa, Alicia N.
author_facet Massa, Alicia N.
Bressano, Marina
Soave, Juan H.
Buteler, Mario I.
Seijo, José Guillermo
Sobolev, Victor S.
Orner, Valerie A.
Oddino, Claudio Marcelo
Soave, Sara J.
Faustinelli, Paola Carmen
de Blas, Francisco Javier
Lamb, Marshall C.
Arias, Renee S.
author_role author
author2 Bressano, Marina
Soave, Juan H.
Buteler, Mario I.
Seijo, José Guillermo
Sobolev, Victor S.
Orner, Valerie A.
Oddino, Claudio Marcelo
Soave, Sara J.
Faustinelli, Paola Carmen
de Blas, Francisco Javier
Lamb, Marshall C.
Arias, Renee S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARACHIS HYPOGAEA
GENETIC INTROGRESSION
PEANUT
PEANUT SMUT
RHAMP ASSAY
SNP GENOTYPING
THECAPHORA FREZII
topic ARACHIS HYPOGAEA
GENETIC INTROGRESSION
PEANUT
PEANUT SMUT
RHAMP ASSAY
SNP GENOTYPING
THECAPHORA FREZII
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical (P < 0.01, bootstrap t-test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers.
Fil: Massa, Alicia N.. National Peanut Research Laboratory; Estados Unidos
Fil: Bressano, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Soave, Juan H.. Criadero El Carmen; Argentina
Fil: Buteler, Mario I.. Criadero El Carmen; Argentina
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sobolev, Victor S.. National Peanut Research Laboratory; Estados Unidos
Fil: Orner, Valerie A.. National Peanut Research Laboratory; Estados Unidos
Fil: Oddino, Claudio Marcelo. Criadero El Carmen; Argentina
Fil: Soave, Sara J.. Criadero El Carmen; Argentina
Fil: Faustinelli, Paola Carmen. National Peanut Research Laboratory; Estados Unidos
Fil: de Blas, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Lamb, Marshall C.. National Peanut Research Laboratory; Estados Unidos
Fil: Arias, Renee S.. National Peanut Research Laboratory; Estados Unidos
description Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical (P < 0.01, bootstrap t-test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/136026
Massa, Alicia N.; Bressano, Marina; Soave, Juan H.; Buteler, Mario I.; Seijo, José Guillermo; et al.; Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study; PeerJ Inc.; PeerJ; 9; 1-2021; 1-18
2167-8359
CONICET Digital
CONICET
url http://hdl.handle.net/11336/136026
identifier_str_mv Massa, Alicia N.; Bressano, Marina; Soave, Juan H.; Buteler, Mario I.; Seijo, José Guillermo; et al.; Genotyping tools and resources to assess peanut germplasm: Smut-resistant landraces as a case study; PeerJ Inc.; PeerJ; 9; 1-2021; 1-18
2167-8359
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/10581/#
info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.10581
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv PeerJ Inc.
publisher.none.fl_str_mv PeerJ Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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