Genetic mapping and QTL analysis for peanut smut resistance

• Autores: de Blas, Francisco Javier; Bruno, Cecilia Ines; Arias, Renee S.; Ballén Taborda, Carolina; Mamaní, Eva Maria Celia; Oddino, Claudio Marcelo; Rosso, Melina; Costero, Beatriz; Bressano, Marina; Soave, Juan H.; Soave, Sara Josefina; Buteler, Mario I.; Seijo, José Guillermo; Massa, Alicia N.
• Año de publicación: 2021
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: ARACHIS; DISEASE RESISTANCE; GROUNDNUT; INTROGRESSION; PEANUT; PEANUT SMUT; QTL; RIL POPULATION
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Background: Peanut smut is a disease caused by the fungus Thecaphora frezii Carranza & Lindquist to which most commercial cultivars in South America are highly susceptible. It is responsible for severely decreased yield and no effective chemical treatment is available to date. However, smut resistance has been identified in wild Arachis species and further transferred to peanut elite cultivars. To identify the genome regions conferring smut resistance within a tetraploid genetic background, this study evaluated a RIL population {susceptible Arachis hypogaea subsp. hypogaea (JS17304-7-B) × resistant synthetic amphidiploid (JS1806) [A. correntina (K 11905) × A. cardenasii (KSSc 36015)] × A. batizocoi (K 9484)4×} segregating for the trait. Results: A SNP based genetic map arranged into 21 linkage groups belonging to the 20 peanut chromosomes was constructed with 1819 markers, spanning a genetic distance of 2531.81 cM. Two consistent quantitative trait loci (QTLs) were identified qSmIA08 and qSmIA02/B02, located on chromosome A08 and A02/B02, respectively. The QTL qSmIA08 at 15.20 cM/5.03 Mbp explained 17.53% of the phenotypic variance, while qSmIA02/B02 at 4.0 cM/3.56 Mbp explained 9.06% of the phenotypic variance. The combined genotypic effects of both QTLs reduced smut incidence by 57% and were stable over the 3 years of evaluation. The genome regions containing the QTLs are rich in genes encoding proteins involved in plant defense, providing new insights into the genetic architecture of peanut smut resistance. Conclusions: A major QTL and a minor QTL identified in this study provide new insights into the genetic architecture of peanut smut resistance that may aid in breeding new varieties resistant to peanut smut.
Fil: de Blas, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Bruno, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Arias, Renee S.. National Peanut Research Laboratory; Estados Unidos
Fil: Ballén Taborda, Carolina. University of Georgia; Estados Unidos
Fil: Mamaní, Eva Maria Celia. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Oddino, Claudio Marcelo. Universidad Nacional de Río Cuarto; Argentina
Fil: Rosso, Melina. No especifíca;
Fil: Costero, Beatriz. Universidad Nacional de Córdoba; Argentina
Fil: Bressano, Marina. Universidad Nacional de Córdoba; Argentina
Fil: Soave, Juan H.. No especifíca;
Fil: Soave, Sara Josefina. No especifíca;
Fil: Buteler, Mario I.. No especifíca;
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Massa, Alicia N.. National Peanut Research Laboratory; Estados Unidos