Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina

Autores
Galisteo, Cristina; Sánchez Porro,Cristina; de la Haba, Rafael R.; López Hermoso, Clara; Fernández, Ana Belén; Farias, Maria Eugenia; Ventosa, Antonio
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genus Salinivibrio belongs to the family Vibrionaceae and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35T, was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35T, including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and in silico DNA?DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%?20% (w/v) NaCl, pH 6?10, and 10?42 °C, with optimum growth at 7.0%?7.5% (w/v) NaCl, pH 8.0, and 37 °C, respectively. Strain S35T was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C16:0, C16:1 ω7c and C16:1 ω6c, and C18:1 ω7c and/or C18:1 ω6c. ANI, GGDC, and core-genome analysis determined that strain S35T constitutes a novel species of the genus Salinivibrio, for which the name Salinivibrio socompensis sp. nov. is proposed. The type strain is S35T (= CECT 9634T = BNM 0535T)
Fil: Galisteo, Cristina. Universidad de Sevilla; España
Fil: Sánchez Porro,Cristina. Universidad de Sevilla; España
Fil: de la Haba, Rafael R.. Universidad de Sevilla; España
Fil: López Hermoso, Clara. Universidad de Sevilla; España
Fil: Fernández, Ana Belén. Universidad de Sevilla; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ventosa, Antonio. Department Of Microbiology And Parasitology, Faculty Of; España
Materia
Extremophiles
Stromatolites
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92085
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92085
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, ArgentinaGalisteo, CristinaSánchez Porro,Cristinade la Haba, Rafael R.López Hermoso, ClaraFernández, Ana BelénFarias, Maria EugeniaVentosa, AntonioExtremophilesStromatoliteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The genus Salinivibrio belongs to the family Vibrionaceae and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35T, was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35T, including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and in silico DNA?DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%?20% (w/v) NaCl, pH 6?10, and 10?42 °C, with optimum growth at 7.0%?7.5% (w/v) NaCl, pH 8.0, and 37 °C, respectively. Strain S35T was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C16:0, C16:1 ω7c and C16:1 ω6c, and C18:1 ω7c and/or C18:1 ω6c. ANI, GGDC, and core-genome analysis determined that strain S35T constitutes a novel species of the genus Salinivibrio, for which the name Salinivibrio socompensis sp. nov. is proposed. The type strain is S35T (= CECT 9634T = BNM 0535T)Fil: Galisteo, Cristina. Universidad de Sevilla; EspañaFil: Sánchez Porro,Cristina. Universidad de Sevilla; EspañaFil: de la Haba, Rafael R.. Universidad de Sevilla; EspañaFil: López Hermoso, Clara. Universidad de Sevilla; EspañaFil: Fernández, Ana Belén. Universidad de Sevilla; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ventosa, Antonio. Department Of Microbiology And Parasitology, Faculty Of; EspañaMDPI2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92085Galisteo, Cristina; Sánchez Porro,Cristina; de la Haba, Rafael R.; López Hermoso, Clara; Fernández, Ana Belén; et al.; Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina; MDPI; Microorganisms; 7; 8; 8-2019; 241-2602076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/7/8/241/pdfinfo:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms7080241info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:04Zoai:ri.conicet.gov.ar:11336/92085instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:04.395CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
title Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
spellingShingle Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
Galisteo, Cristina
Extremophiles
Stromatolites
title_short Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
title_full Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
title_fullStr Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
title_full_unstemmed Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
title_sort Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina
dc.creator.none.fl_str_mv Galisteo, Cristina
Sánchez Porro,Cristina
de la Haba, Rafael R.
López Hermoso, Clara
Fernández, Ana Belén
Farias, Maria Eugenia
Ventosa, Antonio
author Galisteo, Cristina
author_facet Galisteo, Cristina
Sánchez Porro,Cristina
de la Haba, Rafael R.
López Hermoso, Clara
Fernández, Ana Belén
Farias, Maria Eugenia
Ventosa, Antonio
author_role author
author2 Sánchez Porro,Cristina
de la Haba, Rafael R.
López Hermoso, Clara
Fernández, Ana Belén
Farias, Maria Eugenia
Ventosa, Antonio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Extremophiles
Stromatolites
topic Extremophiles
Stromatolites
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The genus Salinivibrio belongs to the family Vibrionaceae and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35T, was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35T, including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and in silico DNA?DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%?20% (w/v) NaCl, pH 6?10, and 10?42 °C, with optimum growth at 7.0%?7.5% (w/v) NaCl, pH 8.0, and 37 °C, respectively. Strain S35T was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C16:0, C16:1 ω7c and C16:1 ω6c, and C18:1 ω7c and/or C18:1 ω6c. ANI, GGDC, and core-genome analysis determined that strain S35T constitutes a novel species of the genus Salinivibrio, for which the name Salinivibrio socompensis sp. nov. is proposed. The type strain is S35T (= CECT 9634T = BNM 0535T)
Fil: Galisteo, Cristina. Universidad de Sevilla; España
Fil: Sánchez Porro,Cristina. Universidad de Sevilla; España
Fil: de la Haba, Rafael R.. Universidad de Sevilla; España
Fil: López Hermoso, Clara. Universidad de Sevilla; España
Fil: Fernández, Ana Belén. Universidad de Sevilla; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ventosa, Antonio. Department Of Microbiology And Parasitology, Faculty Of; España
description The genus Salinivibrio belongs to the family Vibrionaceae and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35T, was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35T, including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and in silico DNA?DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%?20% (w/v) NaCl, pH 6?10, and 10?42 °C, with optimum growth at 7.0%?7.5% (w/v) NaCl, pH 8.0, and 37 °C, respectively. Strain S35T was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C16:0, C16:1 ω7c and C16:1 ω6c, and C18:1 ω7c and/or C18:1 ω6c. ANI, GGDC, and core-genome analysis determined that strain S35T constitutes a novel species of the genus Salinivibrio, for which the name Salinivibrio socompensis sp. nov. is proposed. The type strain is S35T (= CECT 9634T = BNM 0535T)
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92085
Galisteo, Cristina; Sánchez Porro,Cristina; de la Haba, Rafael R.; López Hermoso, Clara; Fernández, Ana Belén; et al.; Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina; MDPI; Microorganisms; 7; 8; 8-2019; 241-260
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92085
identifier_str_mv Galisteo, Cristina; Sánchez Porro,Cristina; de la Haba, Rafael R.; López Hermoso, Clara; Fernández, Ana Belén; et al.; Characterization of Salinivibrio socompensis sp. nov., A new halophilic bacterium isolated from the high-altitude hypersaline lake Socompa, Argentina; MDPI; Microorganisms; 7; 8; 8-2019; 241-260
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/7/8/241/pdf
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms7080241
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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