Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and...

Autores: Bonifacio, Alejo Fabian; Cazenave, Jimena; Bacchetta, Carla; Ballesteros, Maria Laura; Bistoni, Maria de Los Angeles; Amé, María Valeria; Bertrand, Lidwina; Hued, Andrea Cecilia
Año de publicación: 2016
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The Pampean region, an extensive area of South America is continuously impacted by agricultural activities and the pesticides related to them like chlorpyrifos and glyphosate. Both pesticides have been registered in freshwater bodies of the region. One of the most abundant and widely distributed fish species in Pampean streams is Cnesterodon decemmaculatus, which have to cope with this altered scenario. In the present study the toxicity of Clorfox®and Roundup Max®, the commercial formulations of chlorpyrifos and glyphosate, respectively, and their mixture where evaluated using a set of biomarkers at different biological organization levels in fish exposed to relevant environmentally pesticides con-centrations. Somatic indexes such as the condition factor (K), and the hepato-somatic index (HSI), the locomotor activity through the distance traveled and the average speed, the enzymatic activities of acetyl-cholinesterase (AChE) in brain and muscle, catalase (CAT) in muscle and liver, glutathione-S-transferase(GST) in brain, liver, muscle and gills, aspartate amino-transferase (AST), alanine amino-transferase (ALT),AST/ALT ratio and alkaline phosphatase (ALP) in liver were measured on C. decemmaculatus. Adult females were exposed during 6 weeks to the following concentrations: 0.0084 ul/l and 0.00084 ul/l of Clorfox(CF), 0.2 and 2 mg/l of Roundup Max (RM) and all the combinations of these concentrations. The CF exposure caused a decrease in the condition factor and in the locomotor activity parameters and induced an increase brain AChE, liver CAT activity and AST/ALT ratio. On the other hand, the exposure to RM produced a decrease in liver GST, AST/ALT ratio and ALP activity. Finally, some pesticide combinations decrease general condition and liver GST activities, and increase brain GST and liver ALP activities. Different responses in biomarkers were observed in mixtures treatments, reflecting the complex interactions between these toxics and suggesting a suppressive action of RM on CF effects. Since the concentrations we tested are environmentally relevant and the overall fish health conditionwas affected, the presence of these pesticides in freshwater systems could impose a risk for populations by causing deleterious effects on C. decemmaculatus in Pampean region.
Fil: Bonifacio, Alejo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Bistoni, Maria de Los Angeles. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Hued, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Materia: Biomarkers
Bioindicator
Fish
Pesticides
Freshwater Quality
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/47520

Acceder

id	CONICETDig_d1e5b16e26bddac707ab143d7575c9f8
oai_identifier_str	oai:ri.conicet.gov.ar:11336/47520
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixturesBonifacio, Alejo FabianCazenave, JimenaBacchetta, CarlaBallesteros, Maria LauraBistoni, Maria de Los AngelesAmé, María ValeriaBertrand, LidwinaHued, Andrea CeciliaBiomarkersBioindicatorFishPesticidesFreshwater Qualityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Pampean region, an extensive area of South America is continuously impacted by agricultural activities and the pesticides related to them like chlorpyrifos and glyphosate. Both pesticides have been registered in freshwater bodies of the region. One of the most abundant and widely distributed fish species in Pampean streams is Cnesterodon decemmaculatus, which have to cope with this altered scenario. In the present study the toxicity of Clorfox®and Roundup Max®, the commercial formulations of chlorpyrifos and glyphosate, respectively, and their mixture where evaluated using a set of biomarkers at different biological organization levels in fish exposed to relevant environmentally pesticides con-centrations. Somatic indexes such as the condition factor (K), and the hepato-somatic index (HSI), the locomotor activity through the distance traveled and the average speed, the enzymatic activities of acetyl-cholinesterase (AChE) in brain and muscle, catalase (CAT) in muscle and liver, glutathione-S-transferase(GST) in brain, liver, muscle and gills, aspartate amino-transferase (AST), alanine amino-transferase (ALT),AST/ALT ratio and alkaline phosphatase (ALP) in liver were measured on C. decemmaculatus. Adult females were exposed during 6 weeks to the following concentrations: 0.0084 ul/l and 0.00084 ul/l of Clorfox(CF), 0.2 and 2 mg/l of Roundup Max (RM) and all the combinations of these concentrations. The CF exposure caused a decrease in the condition factor and in the locomotor activity parameters and induced an increase brain AChE, liver CAT activity and AST/ALT ratio. On the other hand, the exposure to RM produced a decrease in liver GST, AST/ALT ratio and ALP activity. Finally, some pesticide combinations decrease general condition and liver GST activities, and increase brain GST and liver ALP activities. Different responses in biomarkers were observed in mixtures treatments, reflecting the complex interactions between these toxics and suggesting a suppressive action of RM on CF effects. Since the concentrations we tested are environmentally relevant and the overall fish health conditionwas affected, the presence of these pesticides in freshwater systems could impose a risk for populations by causing deleterious effects on C. decemmaculatus in Pampean region.Fil: Bonifacio, Alejo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Bistoni, Maria de Los Angeles. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Hued, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaElsevier Science2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47520Bonifacio, Alejo Fabian; Cazenave, Jimena; Bacchetta, Carla; Ballesteros, Maria Laura; Bistoni, Maria de Los Angeles; et al.; Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures; Elsevier Science; Ecological Indicators; 67; 8-2016; 88-971470-160XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2016.02.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1470160X16300267info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:25Zoai:ri.conicet.gov.ar:11336/47520instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:25.749CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures
title	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures
spellingShingle	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures Bonifacio, Alejo Fabian Biomarkers Bioindicator Fish Pesticides Freshwater Quality
title_short	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures
title_full	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures
title_fullStr	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures
title_full_unstemmed	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures
title_sort	Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures
dc.creator.none.fl_str_mv	Bonifacio, Alejo Fabian Cazenave, Jimena Bacchetta, Carla Ballesteros, Maria Laura Bistoni, Maria de Los Angeles Amé, María Valeria Bertrand, Lidwina Hued, Andrea Cecilia
author	Bonifacio, Alejo Fabian
author_facet	Bonifacio, Alejo Fabian Cazenave, Jimena Bacchetta, Carla Ballesteros, Maria Laura Bistoni, Maria de Los Angeles Amé, María Valeria Bertrand, Lidwina Hued, Andrea Cecilia
author_role	author
author2	Cazenave, Jimena Bacchetta, Carla Ballesteros, Maria Laura Bistoni, Maria de Los Angeles Amé, María Valeria Bertrand, Lidwina Hued, Andrea Cecilia
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Biomarkers Bioindicator Fish Pesticides Freshwater Quality
topic	Biomarkers Bioindicator Fish Pesticides Freshwater Quality
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	The Pampean region, an extensive area of South America is continuously impacted by agricultural activities and the pesticides related to them like chlorpyrifos and glyphosate. Both pesticides have been registered in freshwater bodies of the region. One of the most abundant and widely distributed fish species in Pampean streams is Cnesterodon decemmaculatus, which have to cope with this altered scenario. In the present study the toxicity of Clorfox®and Roundup Max®, the commercial formulations of chlorpyrifos and glyphosate, respectively, and their mixture where evaluated using a set of biomarkers at different biological organization levels in fish exposed to relevant environmentally pesticides con-centrations. Somatic indexes such as the condition factor (K), and the hepato-somatic index (HSI), the locomotor activity through the distance traveled and the average speed, the enzymatic activities of acetyl-cholinesterase (AChE) in brain and muscle, catalase (CAT) in muscle and liver, glutathione-S-transferase(GST) in brain, liver, muscle and gills, aspartate amino-transferase (AST), alanine amino-transferase (ALT),AST/ALT ratio and alkaline phosphatase (ALP) in liver were measured on C. decemmaculatus. Adult females were exposed during 6 weeks to the following concentrations: 0.0084 ul/l and 0.00084 ul/l of Clorfox(CF), 0.2 and 2 mg/l of Roundup Max (RM) and all the combinations of these concentrations. The CF exposure caused a decrease in the condition factor and in the locomotor activity parameters and induced an increase brain AChE, liver CAT activity and AST/ALT ratio. On the other hand, the exposure to RM produced a decrease in liver GST, AST/ALT ratio and ALP activity. Finally, some pesticide combinations decrease general condition and liver GST activities, and increase brain GST and liver ALP activities. Different responses in biomarkers were observed in mixtures treatments, reflecting the complex interactions between these toxics and suggesting a suppressive action of RM on CF effects. Since the concentrations we tested are environmentally relevant and the overall fish health conditionwas affected, the presence of these pesticides in freshwater systems could impose a risk for populations by causing deleterious effects on C. decemmaculatus in Pampean region. Fil: Bonifacio, Alejo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina Fil: Bistoni, Maria de Los Angeles. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Hued, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
description	The Pampean region, an extensive area of South America is continuously impacted by agricultural activities and the pesticides related to them like chlorpyrifos and glyphosate. Both pesticides have been registered in freshwater bodies of the region. One of the most abundant and widely distributed fish species in Pampean streams is Cnesterodon decemmaculatus, which have to cope with this altered scenario. In the present study the toxicity of Clorfox®and Roundup Max®, the commercial formulations of chlorpyrifos and glyphosate, respectively, and their mixture where evaluated using a set of biomarkers at different biological organization levels in fish exposed to relevant environmentally pesticides con-centrations. Somatic indexes such as the condition factor (K), and the hepato-somatic index (HSI), the locomotor activity through the distance traveled and the average speed, the enzymatic activities of acetyl-cholinesterase (AChE) in brain and muscle, catalase (CAT) in muscle and liver, glutathione-S-transferase(GST) in brain, liver, muscle and gills, aspartate amino-transferase (AST), alanine amino-transferase (ALT),AST/ALT ratio and alkaline phosphatase (ALP) in liver were measured on C. decemmaculatus. Adult females were exposed during 6 weeks to the following concentrations: 0.0084 ul/l and 0.00084 ul/l of Clorfox(CF), 0.2 and 2 mg/l of Roundup Max (RM) and all the combinations of these concentrations. The CF exposure caused a decrease in the condition factor and in the locomotor activity parameters and induced an increase brain AChE, liver CAT activity and AST/ALT ratio. On the other hand, the exposure to RM produced a decrease in liver GST, AST/ALT ratio and ALP activity. Finally, some pesticide combinations decrease general condition and liver GST activities, and increase brain GST and liver ALP activities. Different responses in biomarkers were observed in mixtures treatments, reflecting the complex interactions between these toxics and suggesting a suppressive action of RM on CF effects. Since the concentrations we tested are environmentally relevant and the overall fish health conditionwas affected, the presence of these pesticides in freshwater systems could impose a risk for populations by causing deleterious effects on C. decemmaculatus in Pampean region.
publishDate	2016
dc.date.none.fl_str_mv	2016-08
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/47520 Bonifacio, Alejo Fabian; Cazenave, Jimena; Bacchetta, Carla; Ballesteros, Maria Laura; Bistoni, Maria de Los Angeles; et al.; Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures; Elsevier Science; Ecological Indicators; 67; 8-2016; 88-97 1470-160X CONICET Digital CONICET
url	http://hdl.handle.net/11336/47520
identifier_str_mv	Bonifacio, Alejo Fabian; Cazenave, Jimena; Bacchetta, Carla; Ballesteros, Maria Laura; Bistoni, Maria de Los Angeles; et al.; Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures; Elsevier Science; Ecological Indicators; 67; 8-2016; 88-97 1470-160X CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2016.02.011 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1470160X16300267
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier Science
publisher.none.fl_str_mv	Elsevier Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1842980647252000768
score	12.993085

Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and...

Publicaciones similares