Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae

Autores
Hernandez Morfa, Mirelys; Reinoso Vizcaino, Nicolas Martin; Olivero, Nadia Belén; Zappia, Victoria Eugenia; Cortes, Paulo R.; Jaime, Andrea; Echenique, Jose Ricardo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacterial persisters represent a small subpopulation that tolerates high antibiotic concentrations without acquiring heritable resistance, and it may be generated by environmental factors. Here, we report the first antibiotic persistence mechanism in Streptococcus pneumoniae, which is induced by oxidative stress conditions and allows the pneumococcus to survive in the presence of fluoroquinolones. We demonstrated that fluoroquinolone persistence is prompted by both the impact of growth arrest and the oxidative stress response induced by H2O2 in bacterial cells. This process protected pneumococci against the deleterious effects of high ROS levels induced by fluoroquinolones. Importantly, S. pneumoniae develops persistence during infection, and is dependent on the oxidative stress status of the host cells, indicating that its transient intracellular life contributes to this mechanism. Furthermore, our findings suggest persistence may influence the outcome of antibiotic therapy and be part of a multistep mechanism in the evolution of fluoroquinolone resistance.
Fil: Hernandez Morfa, Mirelys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Reinoso Vizcaino, Nicolas Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Olivero, Nadia Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Zappia, Victoria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cortes, Paulo R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Jaime, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
CIPROFLOXACIN
FLUOROQUINOLONES
HOST-PATHOGEN INTERACTIONS
INTRACELLULAR SURVIVAL
LEVOFLOXACIN
MACROPHAGES
MOXIFLOXACIN
NEUTROPHILS
OXIDATIVE STRESS
PATHOGEN INTERACTIONS
PERSISTENCE
PNEUMOCYTES
STREPTOCOCCUS PNEUMONIAE
STRESS RESPONSE
TRIGGERED-PERSISTENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217369
Acceder
id CONICETDig_d1c71b56a433182d51390571aa0a0a90
oai_identifier_str oai:ri.conicet.gov.ar:11336/217369
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniaeHernandez Morfa, MirelysReinoso Vizcaino, Nicolas MartinOlivero, Nadia BelénZappia, Victoria EugeniaCortes, Paulo R.Jaime, AndreaEchenique, Jose RicardoCIPROFLOXACINFLUOROQUINOLONESHOST-PATHOGEN INTERACTIONSINTRACELLULAR SURVIVALLEVOFLOXACINMACROPHAGESMOXIFLOXACINNEUTROPHILSOXIDATIVE STRESSPATHOGEN INTERACTIONSPERSISTENCEPNEUMOCYTESSTREPTOCOCCUS PNEUMONIAESTRESS RESPONSETRIGGERED-PERSISTENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacterial persisters represent a small subpopulation that tolerates high antibiotic concentrations without acquiring heritable resistance, and it may be generated by environmental factors. Here, we report the first antibiotic persistence mechanism in Streptococcus pneumoniae, which is induced by oxidative stress conditions and allows the pneumococcus to survive in the presence of fluoroquinolones. We demonstrated that fluoroquinolone persistence is prompted by both the impact of growth arrest and the oxidative stress response induced by H2O2 in bacterial cells. This process protected pneumococci against the deleterious effects of high ROS levels induced by fluoroquinolones. Importantly, S. pneumoniae develops persistence during infection, and is dependent on the oxidative stress status of the host cells, indicating that its transient intracellular life contributes to this mechanism. Furthermore, our findings suggest persistence may influence the outcome of antibiotic therapy and be part of a multistep mechanism in the evolution of fluoroquinolone resistance.Fil: Hernandez Morfa, Mirelys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Reinoso Vizcaino, Nicolas Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Olivero, Nadia Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Zappia, Victoria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cortes, Paulo R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Jaime, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaAmerican Society for Microbiology2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217369Hernandez Morfa, Mirelys; Reinoso Vizcaino, Nicolas Martin; Olivero, Nadia Belén; Zappia, Victoria Eugenia; Cortes, Paulo R.; et al.; Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae; American Society for Microbiology; Microbiology Spectrum; 10; 6; 11-2022; 1-142165-0497CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/epub/10.1128/spectrum.04364-22info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.04364-22info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:02Zoai:ri.conicet.gov.ar:11336/217369instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:03.254CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
title Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
spellingShingle Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
Hernandez Morfa, Mirelys
CIPROFLOXACIN
FLUOROQUINOLONES
HOST-PATHOGEN INTERACTIONS
INTRACELLULAR SURVIVAL
LEVOFLOXACIN
MACROPHAGES
MOXIFLOXACIN
NEUTROPHILS
OXIDATIVE STRESS
PATHOGEN INTERACTIONS
PERSISTENCE
PNEUMOCYTES
STREPTOCOCCUS PNEUMONIAE
STRESS RESPONSE
TRIGGERED-PERSISTENCE
title_short Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
title_full Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
title_fullStr Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
title_full_unstemmed Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
title_sort Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
dc.creator.none.fl_str_mv Hernandez Morfa, Mirelys
Reinoso Vizcaino, Nicolas Martin
Olivero, Nadia Belén
Zappia, Victoria Eugenia
Cortes, Paulo R.
Jaime, Andrea
Echenique, Jose Ricardo
author Hernandez Morfa, Mirelys
author_facet Hernandez Morfa, Mirelys
Reinoso Vizcaino, Nicolas Martin
Olivero, Nadia Belén
Zappia, Victoria Eugenia
Cortes, Paulo R.
Jaime, Andrea
Echenique, Jose Ricardo
author_role author
author2 Reinoso Vizcaino, Nicolas Martin
Olivero, Nadia Belén
Zappia, Victoria Eugenia
Cortes, Paulo R.
Jaime, Andrea
Echenique, Jose Ricardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CIPROFLOXACIN
FLUOROQUINOLONES
HOST-PATHOGEN INTERACTIONS
INTRACELLULAR SURVIVAL
LEVOFLOXACIN
MACROPHAGES
MOXIFLOXACIN
NEUTROPHILS
OXIDATIVE STRESS
PATHOGEN INTERACTIONS
PERSISTENCE
PNEUMOCYTES
STREPTOCOCCUS PNEUMONIAE
STRESS RESPONSE
TRIGGERED-PERSISTENCE
topic CIPROFLOXACIN
FLUOROQUINOLONES
HOST-PATHOGEN INTERACTIONS
INTRACELLULAR SURVIVAL
LEVOFLOXACIN
MACROPHAGES
MOXIFLOXACIN
NEUTROPHILS
OXIDATIVE STRESS
PATHOGEN INTERACTIONS
PERSISTENCE
PNEUMOCYTES
STREPTOCOCCUS PNEUMONIAE
STRESS RESPONSE
TRIGGERED-PERSISTENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacterial persisters represent a small subpopulation that tolerates high antibiotic concentrations without acquiring heritable resistance, and it may be generated by environmental factors. Here, we report the first antibiotic persistence mechanism in Streptococcus pneumoniae, which is induced by oxidative stress conditions and allows the pneumococcus to survive in the presence of fluoroquinolones. We demonstrated that fluoroquinolone persistence is prompted by both the impact of growth arrest and the oxidative stress response induced by H2O2 in bacterial cells. This process protected pneumococci against the deleterious effects of high ROS levels induced by fluoroquinolones. Importantly, S. pneumoniae develops persistence during infection, and is dependent on the oxidative stress status of the host cells, indicating that its transient intracellular life contributes to this mechanism. Furthermore, our findings suggest persistence may influence the outcome of antibiotic therapy and be part of a multistep mechanism in the evolution of fluoroquinolone resistance.
Fil: Hernandez Morfa, Mirelys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Reinoso Vizcaino, Nicolas Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Olivero, Nadia Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Zappia, Victoria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cortes, Paulo R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Jaime, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Bacterial persisters represent a small subpopulation that tolerates high antibiotic concentrations without acquiring heritable resistance, and it may be generated by environmental factors. Here, we report the first antibiotic persistence mechanism in Streptococcus pneumoniae, which is induced by oxidative stress conditions and allows the pneumococcus to survive in the presence of fluoroquinolones. We demonstrated that fluoroquinolone persistence is prompted by both the impact of growth arrest and the oxidative stress response induced by H2O2 in bacterial cells. This process protected pneumococci against the deleterious effects of high ROS levels induced by fluoroquinolones. Importantly, S. pneumoniae develops persistence during infection, and is dependent on the oxidative stress status of the host cells, indicating that its transient intracellular life contributes to this mechanism. Furthermore, our findings suggest persistence may influence the outcome of antibiotic therapy and be part of a multistep mechanism in the evolution of fluoroquinolone resistance.
publishDate 2022
dc.date.none.fl_str_mv 2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217369
Hernandez Morfa, Mirelys; Reinoso Vizcaino, Nicolas Martin; Olivero, Nadia Belén; Zappia, Victoria Eugenia; Cortes, Paulo R.; et al.; Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae; American Society for Microbiology; Microbiology Spectrum; 10; 6; 11-2022; 1-14
2165-0497
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217369
identifier_str_mv Hernandez Morfa, Mirelys; Reinoso Vizcaino, Nicolas Martin; Olivero, Nadia Belén; Zappia, Victoria Eugenia; Cortes, Paulo R.; et al.; Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae; American Society for Microbiology; Microbiology Spectrum; 10; 6; 11-2022; 1-14
2165-0497
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/epub/10.1128/spectrum.04364-22
info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.04364-22
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614083357179904
score 13.070432

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