Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats

Autores
Lodi, Federica; Cogolludo, Angel; Duarte, Juan; Moreno, Laura; Coviello, Alfredo; Peral, Maria de Los Angeles; Vera, Rocio; Galisteo, Milagros; Jiménez, Rosario; Tamargo, Juan; Perez Vizcaino, Francisco
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
NADPH oxidase is critically involved in increased blood pressure, vascular hypertrophy, inflammation and endothelial dysfunction in experimental and clinical hypertension. We hypothesized that NADPH oxidase might also play a role in the development of spontaneous aortic tone in spontaneously hypertensive rats (SHR). Wistar Kyoto rats (WKY) were used as normotensive controls. Tone was recorded under isometric conditions. NADPH oxidase activity was measured by both lucigenin luminescence and dihydroethidium fluorescence. p47phox protein was localized by immunohistochemistry. SHR (but not WKY rat) aortae showed spontaneous tone in the absence of exogenous vasoconstrictors as evidenced by a stronger relaxant effect of Ca2+-free sodium nitroprusside solution. This tone was enhanced in endothelium-denuded arteries and was inhibited by superoxide dismutase, apocynin, diphenylene iodonium and quercetin. Aortic NADPH oxidase activity, measured by both lucigenin luminescence and dihydroethidium fluorescence, was increased in SHR compared with WKY rats. Immunohistochemical analysis revealed a strong increase in p47phox expression in the medial layer in SHR. Taken together, the present results indicate that enhanced NADPH oxidase activity and, hence, NADPH driven O2- production, is involved in the spontaneous aortic tone in SHR. This was associated with an increased expression of p47phox in the medial layer of the aorta.
Fil: Lodi, Federica. Universidad Autónoma de Madrid; España
Fil: Cogolludo, Angel. Universidad Autónoma de Madrid; España
Fil: Duarte, Juan. Universidad de Granada; España
Fil: Moreno, Laura. Universidad Autónoma de Madrid; España
Fil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vera, Rocio. Universidad de Granada; España
Fil: Galisteo, Milagros. Universidad de Granada; España
Fil: Jiménez, Rosario. Universidad de Granada; España
Fil: Tamargo, Juan. Universidad Autónoma de Madrid; España
Fil: Perez Vizcaino, Francisco. Universidad Autónoma de Madrid; España
Materia
P47PHOX
SHR (SPONTANEOUSLY HYPERTENSIVE RAT)
SPONTANEOUS TONE
SUPEROXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95115
Acceder
id CONICETDig_d1839d4600a660f78551621f50239e05
oai_identifier_str oai:ri.conicet.gov.ar:11336/95115
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive ratsLodi, FedericaCogolludo, AngelDuarte, JuanMoreno, LauraCoviello, AlfredoPeral, Maria de Los AngelesVera, RocioGalisteo, MilagrosJiménez, RosarioTamargo, JuanPerez Vizcaino, FranciscoP47PHOXSHR (SPONTANEOUSLY HYPERTENSIVE RAT)SPONTANEOUS TONESUPEROXIDEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3NADPH oxidase is critically involved in increased blood pressure, vascular hypertrophy, inflammation and endothelial dysfunction in experimental and clinical hypertension. We hypothesized that NADPH oxidase might also play a role in the development of spontaneous aortic tone in spontaneously hypertensive rats (SHR). Wistar Kyoto rats (WKY) were used as normotensive controls. Tone was recorded under isometric conditions. NADPH oxidase activity was measured by both lucigenin luminescence and dihydroethidium fluorescence. p47phox protein was localized by immunohistochemistry. SHR (but not WKY rat) aortae showed spontaneous tone in the absence of exogenous vasoconstrictors as evidenced by a stronger relaxant effect of Ca2+-free sodium nitroprusside solution. This tone was enhanced in endothelium-denuded arteries and was inhibited by superoxide dismutase, apocynin, diphenylene iodonium and quercetin. Aortic NADPH oxidase activity, measured by both lucigenin luminescence and dihydroethidium fluorescence, was increased in SHR compared with WKY rats. Immunohistochemical analysis revealed a strong increase in p47phox expression in the medial layer in SHR. Taken together, the present results indicate that enhanced NADPH oxidase activity and, hence, NADPH driven O2- production, is involved in the spontaneous aortic tone in SHR. This was associated with an increased expression of p47phox in the medial layer of the aorta.Fil: Lodi, Federica. Universidad Autónoma de Madrid; EspañaFil: Cogolludo, Angel. Universidad Autónoma de Madrid; EspañaFil: Duarte, Juan. Universidad de Granada; EspañaFil: Moreno, Laura. Universidad Autónoma de Madrid; EspañaFil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vera, Rocio. Universidad de Granada; EspañaFil: Galisteo, Milagros. Universidad de Granada; EspañaFil: Jiménez, Rosario. Universidad de Granada; EspañaFil: Tamargo, Juan. Universidad Autónoma de Madrid; EspañaFil: Perez Vizcaino, Francisco. Universidad Autónoma de Madrid; EspañaElsevier Science2006-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95115Lodi, Federica; Cogolludo, Angel; Duarte, Juan; Moreno, Laura; Coviello, Alfredo; et al.; Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats; Elsevier Science; European Journal of Pharmacology; 544; 1-3; 8-2006; 97-1030014-2999CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2006.06.028info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014299906006388info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:08Zoai:ri.conicet.gov.ar:11336/95115instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:09.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
title Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
spellingShingle Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
Lodi, Federica
P47PHOX
SHR (SPONTANEOUSLY HYPERTENSIVE RAT)
SPONTANEOUS TONE
SUPEROXIDE
title_short Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
title_full Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
title_fullStr Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
title_full_unstemmed Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
title_sort Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats
dc.creator.none.fl_str_mv Lodi, Federica
Cogolludo, Angel
Duarte, Juan
Moreno, Laura
Coviello, Alfredo
Peral, Maria de Los Angeles
Vera, Rocio
Galisteo, Milagros
Jiménez, Rosario
Tamargo, Juan
Perez Vizcaino, Francisco
author Lodi, Federica
author_facet Lodi, Federica
Cogolludo, Angel
Duarte, Juan
Moreno, Laura
Coviello, Alfredo
Peral, Maria de Los Angeles
Vera, Rocio
Galisteo, Milagros
Jiménez, Rosario
Tamargo, Juan
Perez Vizcaino, Francisco
author_role author
author2 Cogolludo, Angel
Duarte, Juan
Moreno, Laura
Coviello, Alfredo
Peral, Maria de Los Angeles
Vera, Rocio
Galisteo, Milagros
Jiménez, Rosario
Tamargo, Juan
Perez Vizcaino, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv P47PHOX
SHR (SPONTANEOUSLY HYPERTENSIVE RAT)
SPONTANEOUS TONE
SUPEROXIDE
topic P47PHOX
SHR (SPONTANEOUSLY HYPERTENSIVE RAT)
SPONTANEOUS TONE
SUPEROXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv NADPH oxidase is critically involved in increased blood pressure, vascular hypertrophy, inflammation and endothelial dysfunction in experimental and clinical hypertension. We hypothesized that NADPH oxidase might also play a role in the development of spontaneous aortic tone in spontaneously hypertensive rats (SHR). Wistar Kyoto rats (WKY) were used as normotensive controls. Tone was recorded under isometric conditions. NADPH oxidase activity was measured by both lucigenin luminescence and dihydroethidium fluorescence. p47phox protein was localized by immunohistochemistry. SHR (but not WKY rat) aortae showed spontaneous tone in the absence of exogenous vasoconstrictors as evidenced by a stronger relaxant effect of Ca2+-free sodium nitroprusside solution. This tone was enhanced in endothelium-denuded arteries and was inhibited by superoxide dismutase, apocynin, diphenylene iodonium and quercetin. Aortic NADPH oxidase activity, measured by both lucigenin luminescence and dihydroethidium fluorescence, was increased in SHR compared with WKY rats. Immunohistochemical analysis revealed a strong increase in p47phox expression in the medial layer in SHR. Taken together, the present results indicate that enhanced NADPH oxidase activity and, hence, NADPH driven O2- production, is involved in the spontaneous aortic tone in SHR. This was associated with an increased expression of p47phox in the medial layer of the aorta.
Fil: Lodi, Federica. Universidad Autónoma de Madrid; España
Fil: Cogolludo, Angel. Universidad Autónoma de Madrid; España
Fil: Duarte, Juan. Universidad de Granada; España
Fil: Moreno, Laura. Universidad Autónoma de Madrid; España
Fil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vera, Rocio. Universidad de Granada; España
Fil: Galisteo, Milagros. Universidad de Granada; España
Fil: Jiménez, Rosario. Universidad de Granada; España
Fil: Tamargo, Juan. Universidad Autónoma de Madrid; España
Fil: Perez Vizcaino, Francisco. Universidad Autónoma de Madrid; España
description NADPH oxidase is critically involved in increased blood pressure, vascular hypertrophy, inflammation and endothelial dysfunction in experimental and clinical hypertension. We hypothesized that NADPH oxidase might also play a role in the development of spontaneous aortic tone in spontaneously hypertensive rats (SHR). Wistar Kyoto rats (WKY) were used as normotensive controls. Tone was recorded under isometric conditions. NADPH oxidase activity was measured by both lucigenin luminescence and dihydroethidium fluorescence. p47phox protein was localized by immunohistochemistry. SHR (but not WKY rat) aortae showed spontaneous tone in the absence of exogenous vasoconstrictors as evidenced by a stronger relaxant effect of Ca2+-free sodium nitroprusside solution. This tone was enhanced in endothelium-denuded arteries and was inhibited by superoxide dismutase, apocynin, diphenylene iodonium and quercetin. Aortic NADPH oxidase activity, measured by both lucigenin luminescence and dihydroethidium fluorescence, was increased in SHR compared with WKY rats. Immunohistochemical analysis revealed a strong increase in p47phox expression in the medial layer in SHR. Taken together, the present results indicate that enhanced NADPH oxidase activity and, hence, NADPH driven O2- production, is involved in the spontaneous aortic tone in SHR. This was associated with an increased expression of p47phox in the medial layer of the aorta.
publishDate 2006
dc.date.none.fl_str_mv 2006-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95115
Lodi, Federica; Cogolludo, Angel; Duarte, Juan; Moreno, Laura; Coviello, Alfredo; et al.; Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats; Elsevier Science; European Journal of Pharmacology; 544; 1-3; 8-2006; 97-103
0014-2999
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95115
identifier_str_mv Lodi, Federica; Cogolludo, Angel; Duarte, Juan; Moreno, Laura; Coviello, Alfredo; et al.; Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats; Elsevier Science; European Journal of Pharmacology; 544; 1-3; 8-2006; 97-103
0014-2999
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2006.06.028
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014299906006388
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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