Visualization of fast calcium oscillations in the parafascicular nucleus

Autores
Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the “non-specific” intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems.
Fil: Hyde, James. University Of Arkansas For Medical Sciences; Estados Unidos
Fil: Kezunovic, Nebojsa. University Of Arkansas For Medical Sciences; Estados Unidos
Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Garcia Rill, Edgar. University Of Arkansas For Medical Sciences; Estados Unidos
Materia
Arousal
Gamma Band Activity
Intralaminar Thalamus
Parafascicular
P/Q Type Calcium Channels
N Type Calcium Channels
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20364
Acceder
id CONICETDig_d0c3d290e16e635136a463544254d6ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/20364
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Visualization of fast calcium oscillations in the parafascicular nucleusHyde, JamesKezunovic, NebojsaUrbano Suarez, Francisco JoseGarcia Rill, EdgarArousalGamma Band ActivityIntralaminar ThalamusParafascicularP/Q Type Calcium ChannelsN Type Calcium Channelshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the “non-specific” intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems.Fil: Hyde, James. University Of Arkansas For Medical Sciences; Estados UnidosFil: Kezunovic, Nebojsa. University Of Arkansas For Medical Sciences; Estados UnidosFil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Garcia Rill, Edgar. University Of Arkansas For Medical Sciences; Estados UnidosSpringer2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20364Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Visualization of fast calcium oscillations in the parafascicular nucleus; Springer; Pflugers Archiv-european Journal Of Physiology; 465; 9; 4-2013; 1327-13400031-67681432-2013CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00424-013-1264-6info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00424-013-1264-6info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748156/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:34Zoai:ri.conicet.gov.ar:11336/20364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:34.348CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Visualization of fast calcium oscillations in the parafascicular nucleus
title Visualization of fast calcium oscillations in the parafascicular nucleus
spellingShingle Visualization of fast calcium oscillations in the parafascicular nucleus
Hyde, James
Arousal
Gamma Band Activity
Intralaminar Thalamus
Parafascicular
P/Q Type Calcium Channels
N Type Calcium Channels
title_short Visualization of fast calcium oscillations in the parafascicular nucleus
title_full Visualization of fast calcium oscillations in the parafascicular nucleus
title_fullStr Visualization of fast calcium oscillations in the parafascicular nucleus
title_full_unstemmed Visualization of fast calcium oscillations in the parafascicular nucleus
title_sort Visualization of fast calcium oscillations in the parafascicular nucleus
dc.creator.none.fl_str_mv Hyde, James
Kezunovic, Nebojsa
Urbano Suarez, Francisco Jose
Garcia Rill, Edgar
author Hyde, James
author_facet Hyde, James
Kezunovic, Nebojsa
Urbano Suarez, Francisco Jose
Garcia Rill, Edgar
author_role author
author2 Kezunovic, Nebojsa
Urbano Suarez, Francisco Jose
Garcia Rill, Edgar
author2_role author
author
author
dc.subject.none.fl_str_mv Arousal
Gamma Band Activity
Intralaminar Thalamus
Parafascicular
P/Q Type Calcium Channels
N Type Calcium Channels
topic Arousal
Gamma Band Activity
Intralaminar Thalamus
Parafascicular
P/Q Type Calcium Channels
N Type Calcium Channels
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the “non-specific” intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems.
Fil: Hyde, James. University Of Arkansas For Medical Sciences; Estados Unidos
Fil: Kezunovic, Nebojsa. University Of Arkansas For Medical Sciences; Estados Unidos
Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Garcia Rill, Edgar. University Of Arkansas For Medical Sciences; Estados Unidos
description The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the “non-specific” intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20364
Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Visualization of fast calcium oscillations in the parafascicular nucleus; Springer; Pflugers Archiv-european Journal Of Physiology; 465; 9; 4-2013; 1327-1340
0031-6768
1432-2013
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20364
identifier_str_mv Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Visualization of fast calcium oscillations in the parafascicular nucleus; Springer; Pflugers Archiv-european Journal Of Physiology; 465; 9; 4-2013; 1327-1340
0031-6768
1432-2013
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00424-013-1264-6
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00424-013-1264-6
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748156/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269292804964352
score 13.13397

Publicaciones similares