Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches

Autores
Amorim, Jaime R.; Del Cogliano, Manuel E.; Fernández Brando, Romina Jimena; Bilen, Marcos Fabian; Jesus, Monica R.; Luiz, Wilson B; Palermo, Marina Sandra; Ferreira, Rita C.C.; Servat, Esteban G.; Ghiringhelli, Pablo Daniel; Ferreira, Luis C.S.; Bentancor, Leticia Verónica
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract Shiga toxin (Stx) is considered the main virulence factor in Shiga toxin-producing Escherichia coli (STEC) infections. Previously we reported the expression of biologically active Stx by eukaryotic cells in vitro and in vivo following transfection with plasmids encoding Stx under control of the native bacterial promoter . Since stx genes are present in the genome of lysogenic bacteriophages, here we evaluated the relevance of bacteriophages during STEC infection. We used the non-pathogenic E. coli C600 strain carrying a lysogenic 933W mutant bacteriophage in which the stx operon was replaced by a gene encoding the green fluorescent protein (GFP). Tracking GFP expression using an In Vivo Imaging System (IVIS), we detected fluorescence in liver, kidney, and intestine of mice infected with the recombinant E. coli strain after treatment with ciprofloxacin, which induces the lytic replication and release of bacteriophages. In addition, we showed that chitosan, a linear polysaccharide composed of d-glucosamine residues and with a number of commercial and biomedical uses, had strong anti-bacteriophage effects, as demonstrated at in vitro and in vivo conditions. These findings bring promising perspectives for the prevention and treatment of haemolytic uremic syndrome (HUS) cases.
Fil: Amorim, Jaime R.. Universidade de Sao Paulo; Brasil
Fil: Del Cogliano, Manuel E.. Universidad Nacional de Quilmes; Argentina
Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bilen, Marcos Fabian. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jesus, Monica R.. Universidade de Sao Paulo; Brasil
Fil: Luiz, Wilson B. Universidade de Sao Paulo; Brasil
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ferreira, Rita C.C.. Universidade de Sao Paulo; Brasil
Fil: Servat, Esteban G.. BioSur. Buenos Aires; Argentina
Fil: Ghiringhelli, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Ferreira, Luis C.S.. Universidade de Sao Paulo; Brasil
Fil: Bentancor, Leticia Verónica. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BACTERIOFAGOS
TOXINA SHIGA
SINDROME UREMICO HEMOLITICO
CHITOSAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29492
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29492
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approachesAmorim, Jaime R.Del Cogliano, Manuel E.Fernández Brando, Romina JimenaBilen, Marcos FabianJesus, Monica R.Luiz, Wilson BPalermo, Marina SandraFerreira, Rita C.C.Servat, Esteban G.Ghiringhelli, Pablo DanielFerreira, Luis C.S.Bentancor, Leticia VerónicaBACTERIOFAGOSTOXINA SHIGASINDROME UREMICO HEMOLITICOCHITOSANhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Abstract Shiga toxin (Stx) is considered the main virulence factor in Shiga toxin-producing Escherichia coli (STEC) infections. Previously we reported the expression of biologically active Stx by eukaryotic cells in vitro and in vivo following transfection with plasmids encoding Stx under control of the native bacterial promoter . Since stx genes are present in the genome of lysogenic bacteriophages, here we evaluated the relevance of bacteriophages during STEC infection. We used the non-pathogenic E. coli C600 strain carrying a lysogenic 933W mutant bacteriophage in which the stx operon was replaced by a gene encoding the green fluorescent protein (GFP). Tracking GFP expression using an In Vivo Imaging System (IVIS), we detected fluorescence in liver, kidney, and intestine of mice infected with the recombinant E. coli strain after treatment with ciprofloxacin, which induces the lytic replication and release of bacteriophages. In addition, we showed that chitosan, a linear polysaccharide composed of d-glucosamine residues and with a number of commercial and biomedical uses, had strong anti-bacteriophage effects, as demonstrated at in vitro and in vivo conditions. These findings bring promising perspectives for the prevention and treatment of haemolytic uremic syndrome (HUS) cases.Fil: Amorim, Jaime R.. Universidade de Sao Paulo; BrasilFil: Del Cogliano, Manuel E.. Universidad Nacional de Quilmes; ArgentinaFil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bilen, Marcos Fabian. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jesus, Monica R.. Universidade de Sao Paulo; BrasilFil: Luiz, Wilson B. Universidade de Sao Paulo; BrasilFil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Ferreira, Rita C.C.. Universidade de Sao Paulo; BrasilFil: Servat, Esteban G.. BioSur. Buenos Aires; ArgentinaFil: Ghiringhelli, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Ferreira, Luis C.S.. Universidade de Sao Paulo; BrasilFil: Bentancor, Leticia Verónica. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaF1000Research2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29492Amorim, Jaime R.; Del Cogliano, Manuel E.; Fernández Brando, Romina Jimena; Bilen, Marcos Fabian; Jesus, Monica R.; et al.; Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches; F1000Research; F1000Research; 3; 74; 5-2014; 74-812046-1402CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://f1000research.com/articles/3-74/v2info:eu-repo/semantics/altIdentifier/doi/10.12688/f1000research.3718.2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:52:27Zoai:ri.conicet.gov.ar:11336/29492instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:52:27.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
title Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
spellingShingle Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
Amorim, Jaime R.
BACTERIOFAGOS
TOXINA SHIGA
SINDROME UREMICO HEMOLITICO
CHITOSAN
title_short Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
title_full Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
title_fullStr Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
title_full_unstemmed Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
title_sort Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches
dc.creator.none.fl_str_mv Amorim, Jaime R.
Del Cogliano, Manuel E.
Fernández Brando, Romina Jimena
Bilen, Marcos Fabian
Jesus, Monica R.
Luiz, Wilson B
Palermo, Marina Sandra
Ferreira, Rita C.C.
Servat, Esteban G.
Ghiringhelli, Pablo Daniel
Ferreira, Luis C.S.
Bentancor, Leticia Verónica
author Amorim, Jaime R.
author_facet Amorim, Jaime R.
Del Cogliano, Manuel E.
Fernández Brando, Romina Jimena
Bilen, Marcos Fabian
Jesus, Monica R.
Luiz, Wilson B
Palermo, Marina Sandra
Ferreira, Rita C.C.
Servat, Esteban G.
Ghiringhelli, Pablo Daniel
Ferreira, Luis C.S.
Bentancor, Leticia Verónica
author_role author
author2 Del Cogliano, Manuel E.
Fernández Brando, Romina Jimena
Bilen, Marcos Fabian
Jesus, Monica R.
Luiz, Wilson B
Palermo, Marina Sandra
Ferreira, Rita C.C.
Servat, Esteban G.
Ghiringhelli, Pablo Daniel
Ferreira, Luis C.S.
Bentancor, Leticia Verónica
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIOFAGOS
TOXINA SHIGA
SINDROME UREMICO HEMOLITICO
CHITOSAN
topic BACTERIOFAGOS
TOXINA SHIGA
SINDROME UREMICO HEMOLITICO
CHITOSAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Abstract Shiga toxin (Stx) is considered the main virulence factor in Shiga toxin-producing Escherichia coli (STEC) infections. Previously we reported the expression of biologically active Stx by eukaryotic cells in vitro and in vivo following transfection with plasmids encoding Stx under control of the native bacterial promoter . Since stx genes are present in the genome of lysogenic bacteriophages, here we evaluated the relevance of bacteriophages during STEC infection. We used the non-pathogenic E. coli C600 strain carrying a lysogenic 933W mutant bacteriophage in which the stx operon was replaced by a gene encoding the green fluorescent protein (GFP). Tracking GFP expression using an In Vivo Imaging System (IVIS), we detected fluorescence in liver, kidney, and intestine of mice infected with the recombinant E. coli strain after treatment with ciprofloxacin, which induces the lytic replication and release of bacteriophages. In addition, we showed that chitosan, a linear polysaccharide composed of d-glucosamine residues and with a number of commercial and biomedical uses, had strong anti-bacteriophage effects, as demonstrated at in vitro and in vivo conditions. These findings bring promising perspectives for the prevention and treatment of haemolytic uremic syndrome (HUS) cases.
Fil: Amorim, Jaime R.. Universidade de Sao Paulo; Brasil
Fil: Del Cogliano, Manuel E.. Universidad Nacional de Quilmes; Argentina
Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bilen, Marcos Fabian. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jesus, Monica R.. Universidade de Sao Paulo; Brasil
Fil: Luiz, Wilson B. Universidade de Sao Paulo; Brasil
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ferreira, Rita C.C.. Universidade de Sao Paulo; Brasil
Fil: Servat, Esteban G.. BioSur. Buenos Aires; Argentina
Fil: Ghiringhelli, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Ferreira, Luis C.S.. Universidade de Sao Paulo; Brasil
Fil: Bentancor, Leticia Verónica. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Abstract Shiga toxin (Stx) is considered the main virulence factor in Shiga toxin-producing Escherichia coli (STEC) infections. Previously we reported the expression of biologically active Stx by eukaryotic cells in vitro and in vivo following transfection with plasmids encoding Stx under control of the native bacterial promoter . Since stx genes are present in the genome of lysogenic bacteriophages, here we evaluated the relevance of bacteriophages during STEC infection. We used the non-pathogenic E. coli C600 strain carrying a lysogenic 933W mutant bacteriophage in which the stx operon was replaced by a gene encoding the green fluorescent protein (GFP). Tracking GFP expression using an In Vivo Imaging System (IVIS), we detected fluorescence in liver, kidney, and intestine of mice infected with the recombinant E. coli strain after treatment with ciprofloxacin, which induces the lytic replication and release of bacteriophages. In addition, we showed that chitosan, a linear polysaccharide composed of d-glucosamine residues and with a number of commercial and biomedical uses, had strong anti-bacteriophage effects, as demonstrated at in vitro and in vivo conditions. These findings bring promising perspectives for the prevention and treatment of haemolytic uremic syndrome (HUS) cases.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29492
Amorim, Jaime R.; Del Cogliano, Manuel E.; Fernández Brando, Romina Jimena; Bilen, Marcos Fabian; Jesus, Monica R.; et al.; Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches; F1000Research; F1000Research; 3; 74; 5-2014; 74-81
2046-1402
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29492
identifier_str_mv Amorim, Jaime R.; Del Cogliano, Manuel E.; Fernández Brando, Romina Jimena; Bilen, Marcos Fabian; Jesus, Monica R.; et al.; Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches; F1000Research; F1000Research; 3; 74; 5-2014; 74-81
2046-1402
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://f1000research.com/articles/3-74/v2
info:eu-repo/semantics/altIdentifier/doi/10.12688/f1000research.3718.2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv F1000Research
publisher.none.fl_str_mv F1000Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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