Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis

Autores
Cucher, Marcela Alejandra; Mariconti, Mara; Manciulli, Tommaso; Vola, Ambra; Rosenzvit, Mara Cecilia; Brehm, Klaus; Kamenetzky, Laura; Brunetti, Enrico
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alveolar (AE) and cystic (CE) echinococcosis are two parasitic diseases caused by the tapeworms Echinococcus multilocularis and E. granulosus sensu lato (s. l.), respectively. Currently, AE and CE are mainly diagnosed by means of imaging techniques, serology, and clinical and epidemiological data. However, no viability markers that indicate parasite state during infection are available. Extracellular small RNAs (sRNAs) are short non-coding RNAs that can be secreted by cells through association with extracellular vesicles, proteins, or lipoproteins. Circulating sRNAs can show altered expression in pathological states; hence, they are intensively studied as biomarkers for several diseases. Here, we profiled the sRNA transcriptomes of AE and CE patients to identify novel biomarkers to aid in medical decisions when current diagnostic procedures are inconclusive. For this, endogenous and parasitic sRNAs were analyzed by sRNA sequencing in serum from disease negative, positive, and treated patients and patients harboring a non-parasitic lesion. Consequently, 20 differentially expressed sRNAs associated with AE, CE, and/or non-parasitic lesion were identified. Our results represent an in-depth characterization of the effect E. multilocularis and E. granulosus s. l. exert on the extracellular sRNA landscape in human infections and provide a set of novel candidate biomarkers for both AE and CE detection.
Fil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Mariconti, Mara. San Matteo Hospital Foundation; Italia
Fil: Manciulli, Tommaso. San Matteo Hospital Foundation; Italia
Fil: Vola, Ambra. No especifíca;
Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Brehm, Klaus. Universität Würzburg; Alemania
Fil: Kamenetzky, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brunetti, Enrico. San Matteo Hospital Foundation; Italia
Materia
CIRCULATING
DIAGNOSIS
ECHINOCOCCOSIS
ECHINOCOCCUS
EXTRACELLULAR
MARKER
MICRORNA
SERUM
SMALL RNA
TAPEWORM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227757
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227757
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Circulating Small RNA Profiling of Patients with Alveolar and Cystic EchinococcosisCucher, Marcela AlejandraMariconti, MaraManciulli, TommasoVola, AmbraRosenzvit, Mara CeciliaBrehm, KlausKamenetzky, LauraBrunetti, EnricoCIRCULATINGDIAGNOSISECHINOCOCCOSISECHINOCOCCUSEXTRACELLULARMARKERMICRORNASERUMSMALL RNATAPEWORMhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Alveolar (AE) and cystic (CE) echinococcosis are two parasitic diseases caused by the tapeworms Echinococcus multilocularis and E. granulosus sensu lato (s. l.), respectively. Currently, AE and CE are mainly diagnosed by means of imaging techniques, serology, and clinical and epidemiological data. However, no viability markers that indicate parasite state during infection are available. Extracellular small RNAs (sRNAs) are short non-coding RNAs that can be secreted by cells through association with extracellular vesicles, proteins, or lipoproteins. Circulating sRNAs can show altered expression in pathological states; hence, they are intensively studied as biomarkers for several diseases. Here, we profiled the sRNA transcriptomes of AE and CE patients to identify novel biomarkers to aid in medical decisions when current diagnostic procedures are inconclusive. For this, endogenous and parasitic sRNAs were analyzed by sRNA sequencing in serum from disease negative, positive, and treated patients and patients harboring a non-parasitic lesion. Consequently, 20 differentially expressed sRNAs associated with AE, CE, and/or non-parasitic lesion were identified. Our results represent an in-depth characterization of the effect E. multilocularis and E. granulosus s. l. exert on the extracellular sRNA landscape in human infections and provide a set of novel candidate biomarkers for both AE and CE detection.Fil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Mariconti, Mara. San Matteo Hospital Foundation; ItaliaFil: Manciulli, Tommaso. San Matteo Hospital Foundation; ItaliaFil: Vola, Ambra. No especifíca;Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Brehm, Klaus. Universität Würzburg; AlemaniaFil: Kamenetzky, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brunetti, Enrico. San Matteo Hospital Foundation; ItaliaMDPI2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227757Cucher, Marcela Alejandra; Mariconti, Mara; Manciulli, Tommaso; Vola, Ambra; Rosenzvit, Mara Cecilia; et al.; Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis; MDPI; Biology; 12; 5; 5-2023; 1-202079-7737CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-7737/12/5/715info:eu-repo/semantics/altIdentifier/doi/10.3390/biology12050715info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:16Zoai:ri.conicet.gov.ar:11336/227757instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:17.246CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
title Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
spellingShingle Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
Cucher, Marcela Alejandra
CIRCULATING
DIAGNOSIS
ECHINOCOCCOSIS
ECHINOCOCCUS
EXTRACELLULAR
MARKER
MICRORNA
SERUM
SMALL RNA
TAPEWORM
title_short Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
title_full Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
title_fullStr Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
title_full_unstemmed Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
title_sort Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis
dc.creator.none.fl_str_mv Cucher, Marcela Alejandra
Mariconti, Mara
Manciulli, Tommaso
Vola, Ambra
Rosenzvit, Mara Cecilia
Brehm, Klaus
Kamenetzky, Laura
Brunetti, Enrico
author Cucher, Marcela Alejandra
author_facet Cucher, Marcela Alejandra
Mariconti, Mara
Manciulli, Tommaso
Vola, Ambra
Rosenzvit, Mara Cecilia
Brehm, Klaus
Kamenetzky, Laura
Brunetti, Enrico
author_role author
author2 Mariconti, Mara
Manciulli, Tommaso
Vola, Ambra
Rosenzvit, Mara Cecilia
Brehm, Klaus
Kamenetzky, Laura
Brunetti, Enrico
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CIRCULATING
DIAGNOSIS
ECHINOCOCCOSIS
ECHINOCOCCUS
EXTRACELLULAR
MARKER
MICRORNA
SERUM
SMALL RNA
TAPEWORM
topic CIRCULATING
DIAGNOSIS
ECHINOCOCCOSIS
ECHINOCOCCUS
EXTRACELLULAR
MARKER
MICRORNA
SERUM
SMALL RNA
TAPEWORM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Alveolar (AE) and cystic (CE) echinococcosis are two parasitic diseases caused by the tapeworms Echinococcus multilocularis and E. granulosus sensu lato (s. l.), respectively. Currently, AE and CE are mainly diagnosed by means of imaging techniques, serology, and clinical and epidemiological data. However, no viability markers that indicate parasite state during infection are available. Extracellular small RNAs (sRNAs) are short non-coding RNAs that can be secreted by cells through association with extracellular vesicles, proteins, or lipoproteins. Circulating sRNAs can show altered expression in pathological states; hence, they are intensively studied as biomarkers for several diseases. Here, we profiled the sRNA transcriptomes of AE and CE patients to identify novel biomarkers to aid in medical decisions when current diagnostic procedures are inconclusive. For this, endogenous and parasitic sRNAs were analyzed by sRNA sequencing in serum from disease negative, positive, and treated patients and patients harboring a non-parasitic lesion. Consequently, 20 differentially expressed sRNAs associated with AE, CE, and/or non-parasitic lesion were identified. Our results represent an in-depth characterization of the effect E. multilocularis and E. granulosus s. l. exert on the extracellular sRNA landscape in human infections and provide a set of novel candidate biomarkers for both AE and CE detection.
Fil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Mariconti, Mara. San Matteo Hospital Foundation; Italia
Fil: Manciulli, Tommaso. San Matteo Hospital Foundation; Italia
Fil: Vola, Ambra. No especifíca;
Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Brehm, Klaus. Universität Würzburg; Alemania
Fil: Kamenetzky, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brunetti, Enrico. San Matteo Hospital Foundation; Italia
description Alveolar (AE) and cystic (CE) echinococcosis are two parasitic diseases caused by the tapeworms Echinococcus multilocularis and E. granulosus sensu lato (s. l.), respectively. Currently, AE and CE are mainly diagnosed by means of imaging techniques, serology, and clinical and epidemiological data. However, no viability markers that indicate parasite state during infection are available. Extracellular small RNAs (sRNAs) are short non-coding RNAs that can be secreted by cells through association with extracellular vesicles, proteins, or lipoproteins. Circulating sRNAs can show altered expression in pathological states; hence, they are intensively studied as biomarkers for several diseases. Here, we profiled the sRNA transcriptomes of AE and CE patients to identify novel biomarkers to aid in medical decisions when current diagnostic procedures are inconclusive. For this, endogenous and parasitic sRNAs were analyzed by sRNA sequencing in serum from disease negative, positive, and treated patients and patients harboring a non-parasitic lesion. Consequently, 20 differentially expressed sRNAs associated with AE, CE, and/or non-parasitic lesion were identified. Our results represent an in-depth characterization of the effect E. multilocularis and E. granulosus s. l. exert on the extracellular sRNA landscape in human infections and provide a set of novel candidate biomarkers for both AE and CE detection.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227757
Cucher, Marcela Alejandra; Mariconti, Mara; Manciulli, Tommaso; Vola, Ambra; Rosenzvit, Mara Cecilia; et al.; Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis; MDPI; Biology; 12; 5; 5-2023; 1-20
2079-7737
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227757
identifier_str_mv Cucher, Marcela Alejandra; Mariconti, Mara; Manciulli, Tommaso; Vola, Ambra; Rosenzvit, Mara Cecilia; et al.; Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis; MDPI; Biology; 12; 5; 5-2023; 1-20
2079-7737
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-7737/12/5/715
info:eu-repo/semantics/altIdentifier/doi/10.3390/biology12050715
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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