Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)

Autores
Roma, Dardo Andrés; Cecchini, Maria Eugenia; Tonini, Maria Paula; Capella, Virginia; Aiassa, Delia Elba; Rodriguez, Nancy; Mañas, Fernando Javier
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glyphosate is a systemic, non-selective, pre and post-emergence wide range herbicide. In 2015, IARC classified Glyphosate as “a probable carcinogenic agent for humans”. The aim of this study was to evaluate the cytotoxicity and genotoxicity of the commercial formulation of glyphosate (Roundup® Control Max) at environmentally relevant concentrations and measure the potential effect of this herbicide over the cell capacity to repair DNA damage. HEK293 cells were exposed to 5 concentrations of Roundup® Control Max equivalent to 0.7; 7; 70; 700 and 3,500 μg/L glyphosate acid, for 1, 4 and 24 h. Cytotoxicity was quantified by the Trypan Blue staining method and by the MTT assay, while genotoxicity and evaluation of DNA damage repair kinetics were analyzed through the alkaline comet assay. In all treatments, cell viability was higher than 80%. The three highest glyphosate concentrations—70 μg/L, 700 μg/L, and 3,500 μg/L—increased levels of DNA damage compared to the control at the three exposure times tested. Finally, concerning the kinetics of DNA damage repair, cells initially exposed to 3,500 μg/L of glyphosate for 24 h were unable to repair the breaks in DNA strands even after 4 h of incubation in culture medium. The present study demonstrated for the first time that Roundup® Control Max may induce genetic damage and cause alterations in the DNA repair system in human embryonic kidney cells even at concentrations found in blood and breast milk of people exposed through residues of the herbicide in food, which values have been poorly assessed or not studied yet according to the existent literature.
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cecchini, Maria Eugenia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Tonini, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Aiassa, Delia Elba. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Mañas, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
DNA REPAIR
GENOTOXICITY
ROUNDUP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/226786
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)Roma, Dardo AndrésCecchini, Maria EugeniaTonini, Maria PaulaCapella, VirginiaAiassa, Delia ElbaRodriguez, NancyMañas, Fernando JavierDNA REPAIRGENOTOXICITYROUNDUPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Glyphosate is a systemic, non-selective, pre and post-emergence wide range herbicide. In 2015, IARC classified Glyphosate as “a probable carcinogenic agent for humans”. The aim of this study was to evaluate the cytotoxicity and genotoxicity of the commercial formulation of glyphosate (Roundup® Control Max) at environmentally relevant concentrations and measure the potential effect of this herbicide over the cell capacity to repair DNA damage. HEK293 cells were exposed to 5 concentrations of Roundup® Control Max equivalent to 0.7; 7; 70; 700 and 3,500 μg/L glyphosate acid, for 1, 4 and 24 h. Cytotoxicity was quantified by the Trypan Blue staining method and by the MTT assay, while genotoxicity and evaluation of DNA damage repair kinetics were analyzed through the alkaline comet assay. In all treatments, cell viability was higher than 80%. The three highest glyphosate concentrations—70 μg/L, 700 μg/L, and 3,500 μg/L—increased levels of DNA damage compared to the control at the three exposure times tested. Finally, concerning the kinetics of DNA damage repair, cells initially exposed to 3,500 μg/L of glyphosate for 24 h were unable to repair the breaks in DNA strands even after 4 h of incubation in culture medium. The present study demonstrated for the first time that Roundup® Control Max may induce genetic damage and cause alterations in the DNA repair system in human embryonic kidney cells even at concentrations found in blood and breast milk of people exposed through residues of the herbicide in food, which values have been poorly assessed or not studied yet according to the existent literature.Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Cecchini, Maria Eugenia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Tonini, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaFil: Capella, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Aiassa, Delia Elba. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaFil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaFil: Mañas, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaOxford University Press2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/226786Roma, Dardo Andrés; Cecchini, Maria Eugenia; Tonini, Maria Paula; Capella, Virginia; Aiassa, Delia Elba; et al.; Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max); Oxford University Press; Toxicology Research; 12; 5; 9-2023; 970-9782045-4538CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/toxres/advance-article/doi/10.1093/toxres/tfad089/7287093info:eu-repo/semantics/altIdentifier/doi/10.1093/toxres/tfad089info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:11Zoai:ri.conicet.gov.ar:11336/226786instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:11.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
title Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
spellingShingle Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
Roma, Dardo Andrés
DNA REPAIR
GENOTOXICITY
ROUNDUP
title_short Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
title_full Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
title_fullStr Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
title_full_unstemmed Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
title_sort Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max)
dc.creator.none.fl_str_mv Roma, Dardo Andrés
Cecchini, Maria Eugenia
Tonini, Maria Paula
Capella, Virginia
Aiassa, Delia Elba
Rodriguez, Nancy
Mañas, Fernando Javier
author Roma, Dardo Andrés
author_facet Roma, Dardo Andrés
Cecchini, Maria Eugenia
Tonini, Maria Paula
Capella, Virginia
Aiassa, Delia Elba
Rodriguez, Nancy
Mañas, Fernando Javier
author_role author
author2 Cecchini, Maria Eugenia
Tonini, Maria Paula
Capella, Virginia
Aiassa, Delia Elba
Rodriguez, Nancy
Mañas, Fernando Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DNA REPAIR
GENOTOXICITY
ROUNDUP
topic DNA REPAIR
GENOTOXICITY
ROUNDUP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Glyphosate is a systemic, non-selective, pre and post-emergence wide range herbicide. In 2015, IARC classified Glyphosate as “a probable carcinogenic agent for humans”. The aim of this study was to evaluate the cytotoxicity and genotoxicity of the commercial formulation of glyphosate (Roundup® Control Max) at environmentally relevant concentrations and measure the potential effect of this herbicide over the cell capacity to repair DNA damage. HEK293 cells were exposed to 5 concentrations of Roundup® Control Max equivalent to 0.7; 7; 70; 700 and 3,500 μg/L glyphosate acid, for 1, 4 and 24 h. Cytotoxicity was quantified by the Trypan Blue staining method and by the MTT assay, while genotoxicity and evaluation of DNA damage repair kinetics were analyzed through the alkaline comet assay. In all treatments, cell viability was higher than 80%. The three highest glyphosate concentrations—70 μg/L, 700 μg/L, and 3,500 μg/L—increased levels of DNA damage compared to the control at the three exposure times tested. Finally, concerning the kinetics of DNA damage repair, cells initially exposed to 3,500 μg/L of glyphosate for 24 h were unable to repair the breaks in DNA strands even after 4 h of incubation in culture medium. The present study demonstrated for the first time that Roundup® Control Max may induce genetic damage and cause alterations in the DNA repair system in human embryonic kidney cells even at concentrations found in blood and breast milk of people exposed through residues of the herbicide in food, which values have been poorly assessed or not studied yet according to the existent literature.
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cecchini, Maria Eugenia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Tonini, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Aiassa, Delia Elba. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Mañas, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Glyphosate is a systemic, non-selective, pre and post-emergence wide range herbicide. In 2015, IARC classified Glyphosate as “a probable carcinogenic agent for humans”. The aim of this study was to evaluate the cytotoxicity and genotoxicity of the commercial formulation of glyphosate (Roundup® Control Max) at environmentally relevant concentrations and measure the potential effect of this herbicide over the cell capacity to repair DNA damage. HEK293 cells were exposed to 5 concentrations of Roundup® Control Max equivalent to 0.7; 7; 70; 700 and 3,500 μg/L glyphosate acid, for 1, 4 and 24 h. Cytotoxicity was quantified by the Trypan Blue staining method and by the MTT assay, while genotoxicity and evaluation of DNA damage repair kinetics were analyzed through the alkaline comet assay. In all treatments, cell viability was higher than 80%. The three highest glyphosate concentrations—70 μg/L, 700 μg/L, and 3,500 μg/L—increased levels of DNA damage compared to the control at the three exposure times tested. Finally, concerning the kinetics of DNA damage repair, cells initially exposed to 3,500 μg/L of glyphosate for 24 h were unable to repair the breaks in DNA strands even after 4 h of incubation in culture medium. The present study demonstrated for the first time that Roundup® Control Max may induce genetic damage and cause alterations in the DNA repair system in human embryonic kidney cells even at concentrations found in blood and breast milk of people exposed through residues of the herbicide in food, which values have been poorly assessed or not studied yet according to the existent literature.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/226786
Roma, Dardo Andrés; Cecchini, Maria Eugenia; Tonini, Maria Paula; Capella, Virginia; Aiassa, Delia Elba; et al.; Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max); Oxford University Press; Toxicology Research; 12; 5; 9-2023; 970-978
2045-4538
CONICET Digital
CONICET
url http://hdl.handle.net/11336/226786
identifier_str_mv Roma, Dardo Andrés; Cecchini, Maria Eugenia; Tonini, Maria Paula; Capella, Virginia; Aiassa, Delia Elba; et al.; Toxicity assessment and DNA repair kinetics in HEK293 cells exposed to environmentally relevant concentrations of Glyphosate (Roundup® Control Max); Oxford University Press; Toxicology Research; 12; 5; 9-2023; 970-978
2045-4538
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/toxres/advance-article/doi/10.1093/toxres/tfad089/7287093
info:eu-repo/semantics/altIdentifier/doi/10.1093/toxres/tfad089
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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