Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture

Autores
López González, Evelyn Cecilia; Latorre, María Agustina; Larriera, A.; Siroski, Pablo Ariel; Poletta, Gisela Laura
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Caiman latirostris is one of the two crocodilian species living in Argentina. As a result of agricultural expansion produced in recent years, some populations are exposed to continuous pesticide discharge due to the proximity of its natural geographic distribution to extensive agricultural areas. The aim of this study was to evaluate genotoxicity and the effects on growth of Roundup® (RU; glyphosate based formulation) on C. latirostris hatchlings, considering concentrations commonly applied in crops and subsequently decay of the compound in water through time, as it might occur in the environment. C. latirostris 20 days old, from three different clutches, were exposed to two RU concentrations in plastic containers, during two months. RU concentration was progressively decreased through time, so experimental groups were: treatment 1: 11mg/l (concentration at the beginning) to 2,5mg/l (concentration at the end of experiment), treatment 2: 21mg/l to 5mg/l; and a control without RU. At the end of exposure period, blood samples were obtained and the micronucleus (MN) test applied in erythrocytes as a marker of genotoxicity. Results indicated a significant increase in the frequency of MN (p <0.05) and a tendency to lower growth in the groups exposed to RU compared to the negative control. These results, together with those reported in previous studies; warn about the effect that C. latirostris wild populations continuously exposed to low concentrations of pesticides might be suffering.
Fil: López González, Evelyn Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina
Fil: Latorre, María Agustina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larriera, A.. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina
Fil: Siroski, Pablo Ariel. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Catedra de Toxicologia y Bioquimica Legal; Argentina
Materia
Genotoxicity
Micronucleus Test
Pesticides
Field Concentrations
Glyphosate Decrease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14788
Acceder
id CONICETDig_5a7963ad09faf37973c4ce30ad51d545
oai_identifier_str oai:ri.conicet.gov.ar:11336/14788
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agricultureLópez González, Evelyn CeciliaLatorre, María AgustinaLarriera, A.Siroski, Pablo ArielPoletta, Gisela LauraGenotoxicityMicronucleus TestPesticidesField ConcentrationsGlyphosate Decreasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Caiman latirostris is one of the two crocodilian species living in Argentina. As a result of agricultural expansion produced in recent years, some populations are exposed to continuous pesticide discharge due to the proximity of its natural geographic distribution to extensive agricultural areas. The aim of this study was to evaluate genotoxicity and the effects on growth of Roundup® (RU; glyphosate based formulation) on C. latirostris hatchlings, considering concentrations commonly applied in crops and subsequently decay of the compound in water through time, as it might occur in the environment. C. latirostris 20 days old, from three different clutches, were exposed to two RU concentrations in plastic containers, during two months. RU concentration was progressively decreased through time, so experimental groups were: treatment 1: 11mg/l (concentration at the beginning) to 2,5mg/l (concentration at the end of experiment), treatment 2: 21mg/l to 5mg/l; and a control without RU. At the end of exposure period, blood samples were obtained and the micronucleus (MN) test applied in erythrocytes as a marker of genotoxicity. Results indicated a significant increase in the frequency of MN (p <0.05) and a tendency to lower growth in the groups exposed to RU compared to the negative control. These results, together with those reported in previous studies; warn about the effect that C. latirostris wild populations continuously exposed to low concentrations of pesticides might be suffering.Fil: López González, Evelyn Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); ArgentinaFil: Latorre, María Agustina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larriera, A.. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); ArgentinaFil: Siroski, Pablo Ariel. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Catedra de Toxicologia y Bioquimica Legal; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14788López González, Evelyn Cecilia; Latorre, María Agustina; Larriera, A.; Siroski, Pablo Ariel; Poletta, Gisela Laura; Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture; Elsevier; Pesticide Biochemistry And Physiology; 105; 2; 1-2013; 131-1340048-3575enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2012.12.009info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048357513000047info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:07Zoai:ri.conicet.gov.ar:11336/14788instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:07.4CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
title Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
spellingShingle Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
López González, Evelyn Cecilia
Genotoxicity
Micronucleus Test
Pesticides
Field Concentrations
Glyphosate Decrease
title_short Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
title_full Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
title_fullStr Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
title_full_unstemmed Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
title_sort Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture
dc.creator.none.fl_str_mv López González, Evelyn Cecilia
Latorre, María Agustina
Larriera, A.
Siroski, Pablo Ariel
Poletta, Gisela Laura
author López González, Evelyn Cecilia
author_facet López González, Evelyn Cecilia
Latorre, María Agustina
Larriera, A.
Siroski, Pablo Ariel
Poletta, Gisela Laura
author_role author
author2 Latorre, María Agustina
Larriera, A.
Siroski, Pablo Ariel
Poletta, Gisela Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv Genotoxicity
Micronucleus Test
Pesticides
Field Concentrations
Glyphosate Decrease
topic Genotoxicity
Micronucleus Test
Pesticides
Field Concentrations
Glyphosate Decrease
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Caiman latirostris is one of the two crocodilian species living in Argentina. As a result of agricultural expansion produced in recent years, some populations are exposed to continuous pesticide discharge due to the proximity of its natural geographic distribution to extensive agricultural areas. The aim of this study was to evaluate genotoxicity and the effects on growth of Roundup® (RU; glyphosate based formulation) on C. latirostris hatchlings, considering concentrations commonly applied in crops and subsequently decay of the compound in water through time, as it might occur in the environment. C. latirostris 20 days old, from three different clutches, were exposed to two RU concentrations in plastic containers, during two months. RU concentration was progressively decreased through time, so experimental groups were: treatment 1: 11mg/l (concentration at the beginning) to 2,5mg/l (concentration at the end of experiment), treatment 2: 21mg/l to 5mg/l; and a control without RU. At the end of exposure period, blood samples were obtained and the micronucleus (MN) test applied in erythrocytes as a marker of genotoxicity. Results indicated a significant increase in the frequency of MN (p <0.05) and a tendency to lower growth in the groups exposed to RU compared to the negative control. These results, together with those reported in previous studies; warn about the effect that C. latirostris wild populations continuously exposed to low concentrations of pesticides might be suffering.
Fil: López González, Evelyn Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina
Fil: Latorre, María Agustina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larriera, A.. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina
Fil: Siroski, Pablo Ariel. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoologia Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Catedra de Toxicologia y Bioquimica Legal; Argentina
description Caiman latirostris is one of the two crocodilian species living in Argentina. As a result of agricultural expansion produced in recent years, some populations are exposed to continuous pesticide discharge due to the proximity of its natural geographic distribution to extensive agricultural areas. The aim of this study was to evaluate genotoxicity and the effects on growth of Roundup® (RU; glyphosate based formulation) on C. latirostris hatchlings, considering concentrations commonly applied in crops and subsequently decay of the compound in water through time, as it might occur in the environment. C. latirostris 20 days old, from three different clutches, were exposed to two RU concentrations in plastic containers, during two months. RU concentration was progressively decreased through time, so experimental groups were: treatment 1: 11mg/l (concentration at the beginning) to 2,5mg/l (concentration at the end of experiment), treatment 2: 21mg/l to 5mg/l; and a control without RU. At the end of exposure period, blood samples were obtained and the micronucleus (MN) test applied in erythrocytes as a marker of genotoxicity. Results indicated a significant increase in the frequency of MN (p <0.05) and a tendency to lower growth in the groups exposed to RU compared to the negative control. These results, together with those reported in previous studies; warn about the effect that C. latirostris wild populations continuously exposed to low concentrations of pesticides might be suffering.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14788
López González, Evelyn Cecilia; Latorre, María Agustina; Larriera, A.; Siroski, Pablo Ariel; Poletta, Gisela Laura; Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture; Elsevier; Pesticide Biochemistry And Physiology; 105; 2; 1-2013; 131-134
0048-3575
url http://hdl.handle.net/11336/14788
identifier_str_mv López González, Evelyn Cecilia; Latorre, María Agustina; Larriera, A.; Siroski, Pablo Ariel; Poletta, Gisela Laura; Induction of micronuclei in broad snouted caiman (Caiman latirostris) hatchlings exposed in vivo to Roundup® (glyphosate) concentrations used in agriculture; Elsevier; Pesticide Biochemistry And Physiology; 105; 2; 1-2013; 131-134
0048-3575
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2012.12.009
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048357513000047
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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