Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens

Autores
Sandoval Mosqueda, Ivonne Lizeth; Llorente Bousquets, Adriana; Soto, Carlos; Mercado Márquez, Crisóforo; Fadda, Silvina G.; Del Río García, Juan Carlos
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Considering the objectives of “One Health” and the Sustainable development Goals “Goodhealth and well-being” for the development of effective strategies to apply against bacterial resistance,food safety dangers, and zoonosis risks, this project explored the isolation and identification ofLactobacillus strains from the intestinal tract of recently weaned mice; as well as the assessment ofantibacterial activity against clinical and zoonotic pathogens. For molecular identification, 16SrRNA gene-specific primers were used and, via BLAST-NCBI, 16 Ligilactobacillus murinus, oneLigilactobacillus animalis, and one Streptococcus salivarius strains were identified and registered inGenBank after the confirmation of their identity percentage and the phylogenetic analysis of the16 Ligilactobacillus murinus strains and their association with Ligilactobacillus animalis. The 18 isolatedstrains showed antibacterial activity during agar diffusion tests against Listeria monocytogenes ATCC15313, enteropathogenic Escherichia coli O103, and Campylobacter jejuni ATCC 49943. Electrophoreticand zymographic techniques confirmed the presence of bacteriolytic bands with a relative molecularmass of 107 kDa and another of 24 kDa in Ligilactobacillus murinus strains. UPLC-MS analysis allowedthe identification of a 107 kDa lytic protein as an N-acetylmuramoyl-L-amidase involved in cytolysisand considered a bacteriolytic enzyme with antimicrobial activity. The 24 kDa band displayedsimilarity with a portion of protein with aminopeptidase function. It is expected that these findingswill impact the search for new strains and their metabolites with antibacterial activity as an alternativestrategy to inhibit pathogens associated with major health risks that help your solution.
Fil: Sandoval Mosqueda, Ivonne Lizeth. Universidad Nacional Autónoma de México; México
Fil: Llorente Bousquets, Adriana. Universidad Nacional Autónoma de México; México
Fil: Soto, Carlos. Universidad Nacional Autónoma de México; México
Fil: Mercado Márquez, Crisóforo. Universidad Nacional Autónoma de México; México
Fil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Del Río García, Juan Carlos. Universidad Nacional Autónoma de México; México
Materia
BIOINFORMATICS
LACTIC ACID BACTERIA
ANIMAL FEED
LYCTIC ACTIVITY
LACTOBACILLUS
FOOD-BORNE PATHOGENS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/249802
Acceder
id CONICETDig_cfaa99c9928ee4c7c1fd90a0e9ce2aff
oai_identifier_str oai:ri.conicet.gov.ar:11336/249802
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne PathogensSandoval Mosqueda, Ivonne LizethLlorente Bousquets, AdrianaSoto, CarlosMercado Márquez, CrisóforoFadda, Silvina G.Del Río García, Juan CarlosBIOINFORMATICSLACTIC ACID BACTERIAANIMAL FEEDLYCTIC ACTIVITYLACTOBACILLUSFOOD-BORNE PATHOGENShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Considering the objectives of “One Health” and the Sustainable development Goals “Goodhealth and well-being” for the development of effective strategies to apply against bacterial resistance,food safety dangers, and zoonosis risks, this project explored the isolation and identification ofLactobacillus strains from the intestinal tract of recently weaned mice; as well as the assessment ofantibacterial activity against clinical and zoonotic pathogens. For molecular identification, 16SrRNA gene-specific primers were used and, via BLAST-NCBI, 16 Ligilactobacillus murinus, oneLigilactobacillus animalis, and one Streptococcus salivarius strains were identified and registered inGenBank after the confirmation of their identity percentage and the phylogenetic analysis of the16 Ligilactobacillus murinus strains and their association with Ligilactobacillus animalis. The 18 isolatedstrains showed antibacterial activity during agar diffusion tests against Listeria monocytogenes ATCC15313, enteropathogenic Escherichia coli O103, and Campylobacter jejuni ATCC 49943. Electrophoreticand zymographic techniques confirmed the presence of bacteriolytic bands with a relative molecularmass of 107 kDa and another of 24 kDa in Ligilactobacillus murinus strains. UPLC-MS analysis allowedthe identification of a 107 kDa lytic protein as an N-acetylmuramoyl-L-amidase involved in cytolysisand considered a bacteriolytic enzyme with antimicrobial activity. The 24 kDa band displayedsimilarity with a portion of protein with aminopeptidase function. It is expected that these findingswill impact the search for new strains and their metabolites with antibacterial activity as an alternativestrategy to inhibit pathogens associated with major health risks that help your solution.Fil: Sandoval Mosqueda, Ivonne Lizeth. Universidad Nacional Autónoma de México; MéxicoFil: Llorente Bousquets, Adriana. Universidad Nacional Autónoma de México; MéxicoFil: Soto, Carlos. Universidad Nacional Autónoma de México; MéxicoFil: Mercado Márquez, Crisóforo. Universidad Nacional Autónoma de México; MéxicoFil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Del Río García, Juan Carlos. Universidad Nacional Autónoma de México; MéxicoMultidisciplinary Digital Publishing Institute2023-04-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/249802Sandoval Mosqueda, Ivonne Lizeth; Llorente Bousquets, Adriana; Soto, Carlos; Mercado Márquez, Crisóforo; Fadda, Silvina G.; et al.; Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens; Multidisciplinary Digital Publishing Institute; Microorganisms; 11; 4; 4-4-2023; 1-172076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/11/4/942info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms11040942info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:02Zoai:ri.conicet.gov.ar:11336/249802instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:02.746CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
title Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
spellingShingle Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
Sandoval Mosqueda, Ivonne Lizeth
BIOINFORMATICS
LACTIC ACID BACTERIA
ANIMAL FEED
LYCTIC ACTIVITY
LACTOBACILLUS
FOOD-BORNE PATHOGENS
title_short Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
title_full Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
title_fullStr Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
title_full_unstemmed Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
title_sort Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens
dc.creator.none.fl_str_mv Sandoval Mosqueda, Ivonne Lizeth
Llorente Bousquets, Adriana
Soto, Carlos
Mercado Márquez, Crisóforo
Fadda, Silvina G.
Del Río García, Juan Carlos
author Sandoval Mosqueda, Ivonne Lizeth
author_facet Sandoval Mosqueda, Ivonne Lizeth
Llorente Bousquets, Adriana
Soto, Carlos
Mercado Márquez, Crisóforo
Fadda, Silvina G.
Del Río García, Juan Carlos
author_role author
author2 Llorente Bousquets, Adriana
Soto, Carlos
Mercado Márquez, Crisóforo
Fadda, Silvina G.
Del Río García, Juan Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOINFORMATICS
LACTIC ACID BACTERIA
ANIMAL FEED
LYCTIC ACTIVITY
LACTOBACILLUS
FOOD-BORNE PATHOGENS
topic BIOINFORMATICS
LACTIC ACID BACTERIA
ANIMAL FEED
LYCTIC ACTIVITY
LACTOBACILLUS
FOOD-BORNE PATHOGENS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Considering the objectives of “One Health” and the Sustainable development Goals “Goodhealth and well-being” for the development of effective strategies to apply against bacterial resistance,food safety dangers, and zoonosis risks, this project explored the isolation and identification ofLactobacillus strains from the intestinal tract of recently weaned mice; as well as the assessment ofantibacterial activity against clinical and zoonotic pathogens. For molecular identification, 16SrRNA gene-specific primers were used and, via BLAST-NCBI, 16 Ligilactobacillus murinus, oneLigilactobacillus animalis, and one Streptococcus salivarius strains were identified and registered inGenBank after the confirmation of their identity percentage and the phylogenetic analysis of the16 Ligilactobacillus murinus strains and their association with Ligilactobacillus animalis. The 18 isolatedstrains showed antibacterial activity during agar diffusion tests against Listeria monocytogenes ATCC15313, enteropathogenic Escherichia coli O103, and Campylobacter jejuni ATCC 49943. Electrophoreticand zymographic techniques confirmed the presence of bacteriolytic bands with a relative molecularmass of 107 kDa and another of 24 kDa in Ligilactobacillus murinus strains. UPLC-MS analysis allowedthe identification of a 107 kDa lytic protein as an N-acetylmuramoyl-L-amidase involved in cytolysisand considered a bacteriolytic enzyme with antimicrobial activity. The 24 kDa band displayedsimilarity with a portion of protein with aminopeptidase function. It is expected that these findingswill impact the search for new strains and their metabolites with antibacterial activity as an alternativestrategy to inhibit pathogens associated with major health risks that help your solution.
Fil: Sandoval Mosqueda, Ivonne Lizeth. Universidad Nacional Autónoma de México; México
Fil: Llorente Bousquets, Adriana. Universidad Nacional Autónoma de México; México
Fil: Soto, Carlos. Universidad Nacional Autónoma de México; México
Fil: Mercado Márquez, Crisóforo. Universidad Nacional Autónoma de México; México
Fil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Del Río García, Juan Carlos. Universidad Nacional Autónoma de México; México
description Considering the objectives of “One Health” and the Sustainable development Goals “Goodhealth and well-being” for the development of effective strategies to apply against bacterial resistance,food safety dangers, and zoonosis risks, this project explored the isolation and identification ofLactobacillus strains from the intestinal tract of recently weaned mice; as well as the assessment ofantibacterial activity against clinical and zoonotic pathogens. For molecular identification, 16SrRNA gene-specific primers were used and, via BLAST-NCBI, 16 Ligilactobacillus murinus, oneLigilactobacillus animalis, and one Streptococcus salivarius strains were identified and registered inGenBank after the confirmation of their identity percentage and the phylogenetic analysis of the16 Ligilactobacillus murinus strains and their association with Ligilactobacillus animalis. The 18 isolatedstrains showed antibacterial activity during agar diffusion tests against Listeria monocytogenes ATCC15313, enteropathogenic Escherichia coli O103, and Campylobacter jejuni ATCC 49943. Electrophoreticand zymographic techniques confirmed the presence of bacteriolytic bands with a relative molecularmass of 107 kDa and another of 24 kDa in Ligilactobacillus murinus strains. UPLC-MS analysis allowedthe identification of a 107 kDa lytic protein as an N-acetylmuramoyl-L-amidase involved in cytolysisand considered a bacteriolytic enzyme with antimicrobial activity. The 24 kDa band displayedsimilarity with a portion of protein with aminopeptidase function. It is expected that these findingswill impact the search for new strains and their metabolites with antibacterial activity as an alternativestrategy to inhibit pathogens associated with major health risks that help your solution.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/249802
Sandoval Mosqueda, Ivonne Lizeth; Llorente Bousquets, Adriana; Soto, Carlos; Mercado Márquez, Crisóforo; Fadda, Silvina G.; et al.; Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens; Multidisciplinary Digital Publishing Institute; Microorganisms; 11; 4; 4-4-2023; 1-17
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/249802
identifier_str_mv Sandoval Mosqueda, Ivonne Lizeth; Llorente Bousquets, Adriana; Soto, Carlos; Mercado Márquez, Crisóforo; Fadda, Silvina G.; et al.; Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens; Multidisciplinary Digital Publishing Institute; Microorganisms; 11; 4; 4-4-2023; 1-17
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/11/4/942
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms11040942
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613662265835520
score 13.070432

Publicaciones similares