Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats

Autores
Abregu, Adela Victoria; Genta, Susana Beatriz de Fátima; Sanchez Riera, Alicia Noemi; Sanchez, Sara Serafina del V.
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study was undertaken to investigate the consequences of diabetes on the expression of GM1 and GM2 gangliosides in rat liver. Experimental diabetes was induced by treatment with Streptozotocin (STZ) in adult male Sprague-Dawley rats. Two-dimensional thin-layer chromatography of total ganglioside preparations of liver tissues from STZ-induced diabetic rats showed an increased amount of GM1, while GM2 could not be detected. In order to identify ganglioside expression and corroborate possible changes after short-term diabetes (3 weeks), frozen sections of the liver were stained with two monoclonal antibodies, GMB16 (GM1 specific) and GMB28 (GM2 specific). Although both antibodies were capable of immunostaining the diabetic hepatocytes at the cell surface, strong reactivity was observed for GMB16 while GMB28 developed only a weak labeling. The hepatic ganglioside expression of insulin-stabilized diabetic rats was restored, resembling the profile of normal rats. The important alterations in the expression of GM1 and GM2 gangliosides in short-term diabetes were accompanied by certain microscopic changes in the liver, so that these gangliosides may be useful markers in the detection of early liver diabetic complications.
Fil: Abregu, Adela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Genta, Susana Beatriz de Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sanchez Riera, Alicia Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
DIABETES
GANGLIOSIDES
LIVER
RAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97941
Acceder
id CONICETDig_cf3bc7c4053c5fdb828fe01fdb768fe8
oai_identifier_str oai:ri.conicet.gov.ar:11336/97941
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic ratsAbregu, Adela VictoriaGenta, Susana Beatriz de FátimaSanchez Riera, Alicia NoemiSanchez, Sara Serafina del V.DIABETESGANGLIOSIDESLIVERRAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study was undertaken to investigate the consequences of diabetes on the expression of GM1 and GM2 gangliosides in rat liver. Experimental diabetes was induced by treatment with Streptozotocin (STZ) in adult male Sprague-Dawley rats. Two-dimensional thin-layer chromatography of total ganglioside preparations of liver tissues from STZ-induced diabetic rats showed an increased amount of GM1, while GM2 could not be detected. In order to identify ganglioside expression and corroborate possible changes after short-term diabetes (3 weeks), frozen sections of the liver were stained with two monoclonal antibodies, GMB16 (GM1 specific) and GMB28 (GM2 specific). Although both antibodies were capable of immunostaining the diabetic hepatocytes at the cell surface, strong reactivity was observed for GMB16 while GMB28 developed only a weak labeling. The hepatic ganglioside expression of insulin-stabilized diabetic rats was restored, resembling the profile of normal rats. The important alterations in the expression of GM1 and GM2 gangliosides in short-term diabetes were accompanied by certain microscopic changes in the liver, so that these gangliosides may be useful markers in the detection of early liver diabetic complications.Fil: Abregu, Adela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Genta, Susana Beatriz de Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Sanchez Riera, Alicia Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaWiley Blackwell Publishing, Inc2002-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97941Abregu, Adela Victoria; Genta, Susana Beatriz de Fátima; Sanchez Riera, Alicia Noemi; Sanchez, Sara Serafina del V.; Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats; Wiley Blackwell Publishing, Inc; Hepatology Research; 24; 3; 11-2002; 256-2641386-6346CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1386634602000943info:eu-repo/semantics/altIdentifier/doi/10.1016/S1386-6346(02)00094-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:55Zoai:ri.conicet.gov.ar:11336/97941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:55.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
title Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
spellingShingle Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
Abregu, Adela Victoria
DIABETES
GANGLIOSIDES
LIVER
RAT
title_short Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
title_full Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
title_fullStr Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
title_full_unstemmed Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
title_sort Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats
dc.creator.none.fl_str_mv Abregu, Adela Victoria
Genta, Susana Beatriz de Fátima
Sanchez Riera, Alicia Noemi
Sanchez, Sara Serafina del V.
author Abregu, Adela Victoria
author_facet Abregu, Adela Victoria
Genta, Susana Beatriz de Fátima
Sanchez Riera, Alicia Noemi
Sanchez, Sara Serafina del V.
author_role author
author2 Genta, Susana Beatriz de Fátima
Sanchez Riera, Alicia Noemi
Sanchez, Sara Serafina del V.
author2_role author
author
author
dc.subject.none.fl_str_mv DIABETES
GANGLIOSIDES
LIVER
RAT
topic DIABETES
GANGLIOSIDES
LIVER
RAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study was undertaken to investigate the consequences of diabetes on the expression of GM1 and GM2 gangliosides in rat liver. Experimental diabetes was induced by treatment with Streptozotocin (STZ) in adult male Sprague-Dawley rats. Two-dimensional thin-layer chromatography of total ganglioside preparations of liver tissues from STZ-induced diabetic rats showed an increased amount of GM1, while GM2 could not be detected. In order to identify ganglioside expression and corroborate possible changes after short-term diabetes (3 weeks), frozen sections of the liver were stained with two monoclonal antibodies, GMB16 (GM1 specific) and GMB28 (GM2 specific). Although both antibodies were capable of immunostaining the diabetic hepatocytes at the cell surface, strong reactivity was observed for GMB16 while GMB28 developed only a weak labeling. The hepatic ganglioside expression of insulin-stabilized diabetic rats was restored, resembling the profile of normal rats. The important alterations in the expression of GM1 and GM2 gangliosides in short-term diabetes were accompanied by certain microscopic changes in the liver, so that these gangliosides may be useful markers in the detection of early liver diabetic complications.
Fil: Abregu, Adela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Genta, Susana Beatriz de Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sanchez Riera, Alicia Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description This study was undertaken to investigate the consequences of diabetes on the expression of GM1 and GM2 gangliosides in rat liver. Experimental diabetes was induced by treatment with Streptozotocin (STZ) in adult male Sprague-Dawley rats. Two-dimensional thin-layer chromatography of total ganglioside preparations of liver tissues from STZ-induced diabetic rats showed an increased amount of GM1, while GM2 could not be detected. In order to identify ganglioside expression and corroborate possible changes after short-term diabetes (3 weeks), frozen sections of the liver were stained with two monoclonal antibodies, GMB16 (GM1 specific) and GMB28 (GM2 specific). Although both antibodies were capable of immunostaining the diabetic hepatocytes at the cell surface, strong reactivity was observed for GMB16 while GMB28 developed only a weak labeling. The hepatic ganglioside expression of insulin-stabilized diabetic rats was restored, resembling the profile of normal rats. The important alterations in the expression of GM1 and GM2 gangliosides in short-term diabetes were accompanied by certain microscopic changes in the liver, so that these gangliosides may be useful markers in the detection of early liver diabetic complications.
publishDate 2002
dc.date.none.fl_str_mv 2002-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97941
Abregu, Adela Victoria; Genta, Susana Beatriz de Fátima; Sanchez Riera, Alicia Noemi; Sanchez, Sara Serafina del V.; Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats; Wiley Blackwell Publishing, Inc; Hepatology Research; 24; 3; 11-2002; 256-264
1386-6346
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97941
identifier_str_mv Abregu, Adela Victoria; Genta, Susana Beatriz de Fátima; Sanchez Riera, Alicia Noemi; Sanchez, Sara Serafina del V.; Immunohistochemical detection of hepatic GM1 and GM2 gangliosides in streptozotocin-induced diabetic rats; Wiley Blackwell Publishing, Inc; Hepatology Research; 24; 3; 11-2002; 256-264
1386-6346
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1386634602000943
info:eu-repo/semantics/altIdentifier/doi/10.1016/S1386-6346(02)00094-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268761826000896
score 13.13397

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