Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel

Autores
Leonhard, Victoria; Alasino, Roxana Valeria; Bianco, Ismael Dario; Beltramo, Dante Miguel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In our previous work we showed that taxanes (paclitaxel (Ptx) and docetaxel(Dtx)) can be spontaneously loaded into monosialoganglioside (GM1) nanomicelles, increasing their water solubility about 6,000 times, to render stable water soluble formulations that could be used as a novel strategy to deliver drugs in cancer. Here, we describe the hydrophobic interaction of Human Serum Albumin (HSA) with GM1 micelles loaded with Ptx, as a strategy that could improve tumour drug accumulation. This interaction is regulated by conditions such pH and temperature and generates ternary complexes GM1/Ptx/HSA with sizes around 19 to 24 nm and hydrodynamic radius equivalent to a globular protein of 140-180 kDa. These mixed micelles were stable in solution for at least 40 days and also upon freeze-thawing or lyophilization-solubilization cycles. The results of in vitro assays showed that the nano-structures developed are taken up by cell cultures with an antimitotic activity of Ptx on tumoral and nontumoral cell lines that was similar to that observed with the free drug in DMSO solution.
Fil: Leonhard, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia En Productos y Procesos de Córdoba. Laboratorio de Biotecnología; Argentina
Fil: Alasino, Roxana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bianco, Ismael Dario. Universidad Nacional de La Rioja; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beltramo, Dante Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba; Argentina
Materia
ALBUMIN
DRUG-DELIVERY
GANGLIOSIDES
MICELLES
PACLITAXEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199209
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199209
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxelLeonhard, VictoriaAlasino, Roxana ValeriaBianco, Ismael DarioBeltramo, Dante MiguelALBUMINDRUG-DELIVERYGANGLIOSIDESMICELLESPACLITAXELhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2In our previous work we showed that taxanes (paclitaxel (Ptx) and docetaxel(Dtx)) can be spontaneously loaded into monosialoganglioside (GM1) nanomicelles, increasing their water solubility about 6,000 times, to render stable water soluble formulations that could be used as a novel strategy to deliver drugs in cancer. Here, we describe the hydrophobic interaction of Human Serum Albumin (HSA) with GM1 micelles loaded with Ptx, as a strategy that could improve tumour drug accumulation. This interaction is regulated by conditions such pH and temperature and generates ternary complexes GM1/Ptx/HSA with sizes around 19 to 24 nm and hydrodynamic radius equivalent to a globular protein of 140-180 kDa. These mixed micelles were stable in solution for at least 40 days and also upon freeze-thawing or lyophilization-solubilization cycles. The results of in vitro assays showed that the nano-structures developed are taken up by cell cultures with an antimitotic activity of Ptx on tumoral and nontumoral cell lines that was similar to that observed with the free drug in DMSO solution.Fil: Leonhard, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia En Productos y Procesos de Córdoba. Laboratorio de Biotecnología; ArgentinaFil: Alasino, Roxana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bianco, Ismael Dario. Universidad Nacional de La Rioja; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Beltramo, Dante Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba; ArgentinaWalsh Medical Media2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199209Leonhard, Victoria; Alasino, Roxana Valeria; Bianco, Ismael Dario; Beltramo, Dante Miguel; Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel; Walsh Medical Media; Journal of Nanomedicine and Nanotechnology; 4; 1; 1-2013; 159-1652157-7439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7439.1000159info:eu-repo/semantics/altIdentifier/url/https://www.walshmedicalmedia.com/archive/jnmnt-volume-4-issue-1-year-2013.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:07Zoai:ri.conicet.gov.ar:11336/199209instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:07.459CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
title Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
spellingShingle Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
Leonhard, Victoria
ALBUMIN
DRUG-DELIVERY
GANGLIOSIDES
MICELLES
PACLITAXEL
title_short Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
title_full Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
title_fullStr Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
title_full_unstemmed Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
title_sort Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel
dc.creator.none.fl_str_mv Leonhard, Victoria
Alasino, Roxana Valeria
Bianco, Ismael Dario
Beltramo, Dante Miguel
author Leonhard, Victoria
author_facet Leonhard, Victoria
Alasino, Roxana Valeria
Bianco, Ismael Dario
Beltramo, Dante Miguel
author_role author
author2 Alasino, Roxana Valeria
Bianco, Ismael Dario
Beltramo, Dante Miguel
author2_role author
author
author
dc.subject.none.fl_str_mv ALBUMIN
DRUG-DELIVERY
GANGLIOSIDES
MICELLES
PACLITAXEL
topic ALBUMIN
DRUG-DELIVERY
GANGLIOSIDES
MICELLES
PACLITAXEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In our previous work we showed that taxanes (paclitaxel (Ptx) and docetaxel(Dtx)) can be spontaneously loaded into monosialoganglioside (GM1) nanomicelles, increasing their water solubility about 6,000 times, to render stable water soluble formulations that could be used as a novel strategy to deliver drugs in cancer. Here, we describe the hydrophobic interaction of Human Serum Albumin (HSA) with GM1 micelles loaded with Ptx, as a strategy that could improve tumour drug accumulation. This interaction is regulated by conditions such pH and temperature and generates ternary complexes GM1/Ptx/HSA with sizes around 19 to 24 nm and hydrodynamic radius equivalent to a globular protein of 140-180 kDa. These mixed micelles were stable in solution for at least 40 days and also upon freeze-thawing or lyophilization-solubilization cycles. The results of in vitro assays showed that the nano-structures developed are taken up by cell cultures with an antimitotic activity of Ptx on tumoral and nontumoral cell lines that was similar to that observed with the free drug in DMSO solution.
Fil: Leonhard, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia En Productos y Procesos de Córdoba. Laboratorio de Biotecnología; Argentina
Fil: Alasino, Roxana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bianco, Ismael Dario. Universidad Nacional de La Rioja; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beltramo, Dante Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba; Argentina
description In our previous work we showed that taxanes (paclitaxel (Ptx) and docetaxel(Dtx)) can be spontaneously loaded into monosialoganglioside (GM1) nanomicelles, increasing their water solubility about 6,000 times, to render stable water soluble formulations that could be used as a novel strategy to deliver drugs in cancer. Here, we describe the hydrophobic interaction of Human Serum Albumin (HSA) with GM1 micelles loaded with Ptx, as a strategy that could improve tumour drug accumulation. This interaction is regulated by conditions such pH and temperature and generates ternary complexes GM1/Ptx/HSA with sizes around 19 to 24 nm and hydrodynamic radius equivalent to a globular protein of 140-180 kDa. These mixed micelles were stable in solution for at least 40 days and also upon freeze-thawing or lyophilization-solubilization cycles. The results of in vitro assays showed that the nano-structures developed are taken up by cell cultures with an antimitotic activity of Ptx on tumoral and nontumoral cell lines that was similar to that observed with the free drug in DMSO solution.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199209
Leonhard, Victoria; Alasino, Roxana Valeria; Bianco, Ismael Dario; Beltramo, Dante Miguel; Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel; Walsh Medical Media; Journal of Nanomedicine and Nanotechnology; 4; 1; 1-2013; 159-165
2157-7439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199209
identifier_str_mv Leonhard, Victoria; Alasino, Roxana Valeria; Bianco, Ismael Dario; Beltramo, Dante Miguel; Selective binding of albumin to Gm1 ganglioside micelles containing paclitaxel; Walsh Medical Media; Journal of Nanomedicine and Nanotechnology; 4; 1; 1-2013; 159-165
2157-7439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7439.1000159
info:eu-repo/semantics/altIdentifier/url/https://www.walshmedicalmedia.com/archive/jnmnt-volume-4-issue-1-year-2013.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Walsh Medical Media
publisher.none.fl_str_mv Walsh Medical Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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