Urodilatin and dopamine: A new interaction in the kidney

Autores
Citarella, Marisa R.; Choi, Marcelo Roberto; Gironacci, Mariela Mercedes; Medici, Cecilia; Correa, Alicia Haydee; Fernandez, Belisario Enrique
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since renal natriuretic peptide urodilatin (URO) exerts similar natriuretic and diuretic actions to those of atrial natriuretic factor (ANF), we hypothesized that URO regulates renal dopamine (DA) availability, contributing  to Na+, K+-ATPase inhibition. URO (1?100 nM) increased 3H-DA uptake in outer and juxtamedullar renal  cortex and medulla slices from Sprague Dawley rats. Hydrocortisone blocked URO-stimulated DA  uptake, demonstrating that DA uptake was extraneuronal. The natriuretic peptide receptor type A antagonist anantin blocked URO-dependent increase of 3H-DA uptake, while the natriuretic peptide receptor type C agonist ANF -amide did not modify URO effect on DA uptake, suggesting that only  natriuretic receptors type A are involved. Co-incubation of URO and ANF did not show additive effects on DA uptake. To test whether URO effect involves changes in Na+, K+-ATPase activity we performed experiments in renal cortex samples of  rats with DA synthesis and neuronal uptake inhibited by carbidopa and nomifensine, respectively. When endogenous DA synthesis was inhibited, URO or DA decreased Na+, K+-ATPase activity. URO and DA added together, further decreased Na+, K+-ATPase activity showing an additive effect on the sodium pump. Moreover, hydrocortisone reversed URO-DA over-inhibition of the enzyme, confirming that this inhibition is closely related to URO-stimulation on renal DA uptake. URO and DA could act via a common intracellular pathway to decrease sodium and water tubular reabsorption, contributing to its natriuretic and diuretic effects. 
Fil: Citarella, Marisa R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Choi, Marcelo Roberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Medici, Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Correa, Alicia Haydee. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Materia
renal
ANF
Natriuretic peptide receptor type A
Na+, K+-ATPase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113819
Acceder
id CONICETDig_cf16e758fa61f4219984484c80593edd
oai_identifier_str oai:ri.conicet.gov.ar:11336/113819
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Urodilatin and dopamine: A new interaction in the kidneyCitarella, Marisa R.Choi, Marcelo RobertoGironacci, Mariela MercedesMedici, CeciliaCorrea, Alicia HaydeeFernandez, Belisario EnriquerenalANFNatriuretic peptide receptor type ANa+, K+-ATPasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Since renal natriuretic peptide urodilatin (URO) exerts similar natriuretic and diuretic actions to those of atrial natriuretic factor (ANF), we hypothesized that URO regulates renal dopamine (DA) availability, contributing  to Na+, K+-ATPase inhibition. URO (1?100 nM) increased 3H-DA uptake in outer and juxtamedullar renal  cortex and medulla slices from Sprague Dawley rats. Hydrocortisone blocked URO-stimulated DA  uptake, demonstrating that DA uptake was extraneuronal. The natriuretic peptide receptor type A antagonist anantin blocked URO-dependent increase of 3H-DA uptake, while the natriuretic peptide receptor type C agonist ANF -amide did not modify URO effect on DA uptake, suggesting that only  natriuretic receptors type A are involved. Co-incubation of URO and ANF did not show additive effects on DA uptake. To test whether URO effect involves changes in Na+, K+-ATPase activity we performed experiments in renal cortex samples of  rats with DA synthesis and neuronal uptake inhibited by carbidopa and nomifensine, respectively. When endogenous DA synthesis was inhibited, URO or DA decreased Na+, K+-ATPase activity. URO and DA added together, further decreased Na+, K+-ATPase activity showing an additive effect on the sodium pump. Moreover, hydrocortisone reversed URO-DA over-inhibition of the enzyme, confirming that this inhibition is closely related to URO-stimulation on renal DA uptake. URO and DA could act via a common intracellular pathway to decrease sodium and water tubular reabsorption, contributing to its natriuretic and diuretic effects. Fil: Citarella, Marisa R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Choi, Marcelo Roberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Medici, Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Correa, Alicia Haydee. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaElsevier Science2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113819Citarella, Marisa R.; Choi, Marcelo Roberto; Gironacci, Mariela Mercedes; Medici, Cecilia; Correa, Alicia Haydee; et al.; Urodilatin and dopamine: A new interaction in the kidney; Elsevier Science; Regulatory Peptides; 153; 1-3; 2-2009; 19-240167-0115CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0167011508001973info:eu-repo/semantics/altIdentifier/doi/10.1016/j.regpep.2008.11.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:41Zoai:ri.conicet.gov.ar:11336/113819instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:41.504CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Urodilatin and dopamine: A new interaction in the kidney
title Urodilatin and dopamine: A new interaction in the kidney
spellingShingle Urodilatin and dopamine: A new interaction in the kidney
Citarella, Marisa R.
renal
ANF
Natriuretic peptide receptor type A
Na+, K+-ATPase
title_short Urodilatin and dopamine: A new interaction in the kidney
title_full Urodilatin and dopamine: A new interaction in the kidney
title_fullStr Urodilatin and dopamine: A new interaction in the kidney
title_full_unstemmed Urodilatin and dopamine: A new interaction in the kidney
title_sort Urodilatin and dopamine: A new interaction in the kidney
dc.creator.none.fl_str_mv Citarella, Marisa R.
Choi, Marcelo Roberto
Gironacci, Mariela Mercedes
Medici, Cecilia
Correa, Alicia Haydee
Fernandez, Belisario Enrique
author Citarella, Marisa R.
author_facet Citarella, Marisa R.
Choi, Marcelo Roberto
Gironacci, Mariela Mercedes
Medici, Cecilia
Correa, Alicia Haydee
Fernandez, Belisario Enrique
author_role author
author2 Choi, Marcelo Roberto
Gironacci, Mariela Mercedes
Medici, Cecilia
Correa, Alicia Haydee
Fernandez, Belisario Enrique
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv renal
ANF
Natriuretic peptide receptor type A
Na+, K+-ATPase
topic renal
ANF
Natriuretic peptide receptor type A
Na+, K+-ATPase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Since renal natriuretic peptide urodilatin (URO) exerts similar natriuretic and diuretic actions to those of atrial natriuretic factor (ANF), we hypothesized that URO regulates renal dopamine (DA) availability, contributing  to Na+, K+-ATPase inhibition. URO (1?100 nM) increased 3H-DA uptake in outer and juxtamedullar renal  cortex and medulla slices from Sprague Dawley rats. Hydrocortisone blocked URO-stimulated DA  uptake, demonstrating that DA uptake was extraneuronal. The natriuretic peptide receptor type A antagonist anantin blocked URO-dependent increase of 3H-DA uptake, while the natriuretic peptide receptor type C agonist ANF -amide did not modify URO effect on DA uptake, suggesting that only  natriuretic receptors type A are involved. Co-incubation of URO and ANF did not show additive effects on DA uptake. To test whether URO effect involves changes in Na+, K+-ATPase activity we performed experiments in renal cortex samples of  rats with DA synthesis and neuronal uptake inhibited by carbidopa and nomifensine, respectively. When endogenous DA synthesis was inhibited, URO or DA decreased Na+, K+-ATPase activity. URO and DA added together, further decreased Na+, K+-ATPase activity showing an additive effect on the sodium pump. Moreover, hydrocortisone reversed URO-DA over-inhibition of the enzyme, confirming that this inhibition is closely related to URO-stimulation on renal DA uptake. URO and DA could act via a common intracellular pathway to decrease sodium and water tubular reabsorption, contributing to its natriuretic and diuretic effects. 
Fil: Citarella, Marisa R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Choi, Marcelo Roberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Medici, Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Correa, Alicia Haydee. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
description Since renal natriuretic peptide urodilatin (URO) exerts similar natriuretic and diuretic actions to those of atrial natriuretic factor (ANF), we hypothesized that URO regulates renal dopamine (DA) availability, contributing  to Na+, K+-ATPase inhibition. URO (1?100 nM) increased 3H-DA uptake in outer and juxtamedullar renal  cortex and medulla slices from Sprague Dawley rats. Hydrocortisone blocked URO-stimulated DA  uptake, demonstrating that DA uptake was extraneuronal. The natriuretic peptide receptor type A antagonist anantin blocked URO-dependent increase of 3H-DA uptake, while the natriuretic peptide receptor type C agonist ANF -amide did not modify URO effect on DA uptake, suggesting that only  natriuretic receptors type A are involved. Co-incubation of URO and ANF did not show additive effects on DA uptake. To test whether URO effect involves changes in Na+, K+-ATPase activity we performed experiments in renal cortex samples of  rats with DA synthesis and neuronal uptake inhibited by carbidopa and nomifensine, respectively. When endogenous DA synthesis was inhibited, URO or DA decreased Na+, K+-ATPase activity. URO and DA added together, further decreased Na+, K+-ATPase activity showing an additive effect on the sodium pump. Moreover, hydrocortisone reversed URO-DA over-inhibition of the enzyme, confirming that this inhibition is closely related to URO-stimulation on renal DA uptake. URO and DA could act via a common intracellular pathway to decrease sodium and water tubular reabsorption, contributing to its natriuretic and diuretic effects. 
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113819
Citarella, Marisa R.; Choi, Marcelo Roberto; Gironacci, Mariela Mercedes; Medici, Cecilia; Correa, Alicia Haydee; et al.; Urodilatin and dopamine: A new interaction in the kidney; Elsevier Science; Regulatory Peptides; 153; 1-3; 2-2009; 19-24
0167-0115
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113819
identifier_str_mv Citarella, Marisa R.; Choi, Marcelo Roberto; Gironacci, Mariela Mercedes; Medici, Cecilia; Correa, Alicia Haydee; et al.; Urodilatin and dopamine: A new interaction in the kidney; Elsevier Science; Regulatory Peptides; 153; 1-3; 2-2009; 19-24
0167-0115
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0167011508001973
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.regpep.2008.11.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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