Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis

Autores
Kuo, Dian Han; Szczupak, Lidia; Weisblat, David A.; Portiansky, Enrique Leo; Winchell, Christopher J.; Lee, Jun Ru; Tsai, Fu Yu
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The analysis of how neural circuits function in individuals and change during evolution is simplified by the existence of neurons identified as homologous within and across species. Invertebrates, including leeches, have been used for these purposes in part because their nervous systems comprise a high proportion of identified neurons, but technical limitations make it challenging to assess the full extent to which assumptions of stereotypy hold true. Here, we introduce Minos plasmid-mediated transgenesis as a tool for introducing transgenes into the embryos of the leech Helobdella austinensis (Spiralia; Lophotrochozoa; Annelida; Clitellata; Hirudinida; Glossiphoniidae). We identified an enhancer driving pan-neuronal expression of markers, including histone2B: mCherry, which allowed us to enumerate neurons in segmental ganglia. Unexpectedly, we found that the segmental ganglia of adult transgenic H. austinensis contain fewer and more variable numbers of neurons than in previously examined leech species.
Fil: Kuo, Dian Han. National Taiwan University; China
Fil: Szczupak, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Weisblat, David A.. University of California at Berkeley; Estados Unidos
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Winchell, Christopher J.. University of California at Berkeley; Estados Unidos
Fil: Lee, Jun Ru. National Taiwan University; China
Fil: Tsai, Fu Yu. National Taiwan University; China
Materia
Sanguijuela
Neruonas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261226
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensisKuo, Dian HanSzczupak, LidiaWeisblat, David A.Portiansky, Enrique LeoWinchell, Christopher J.Lee, Jun RuTsai, Fu YuSanguijuelaNeruonashttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The analysis of how neural circuits function in individuals and change during evolution is simplified by the existence of neurons identified as homologous within and across species. Invertebrates, including leeches, have been used for these purposes in part because their nervous systems comprise a high proportion of identified neurons, but technical limitations make it challenging to assess the full extent to which assumptions of stereotypy hold true. Here, we introduce Minos plasmid-mediated transgenesis as a tool for introducing transgenes into the embryos of the leech Helobdella austinensis (Spiralia; Lophotrochozoa; Annelida; Clitellata; Hirudinida; Glossiphoniidae). We identified an enhancer driving pan-neuronal expression of markers, including histone2B: mCherry, which allowed us to enumerate neurons in segmental ganglia. Unexpectedly, we found that the segmental ganglia of adult transgenic H. austinensis contain fewer and more variable numbers of neurons than in previously examined leech species.Fil: Kuo, Dian Han. National Taiwan University; ChinaFil: Szczupak, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Weisblat, David A.. University of California at Berkeley; Estados UnidosFil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Winchell, Christopher J.. University of California at Berkeley; Estados UnidosFil: Lee, Jun Ru. National Taiwan University; ChinaFil: Tsai, Fu Yu. National Taiwan University; ChinaCompany of Biologists2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261226Kuo, Dian Han; Szczupak, Lidia; Weisblat, David A.; Portiansky, Enrique Leo; Winchell, Christopher J.; et al.; Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis; Company of Biologists; Journal of Experimental Biology; 227; 14; 7-2024; 1-150022-0949CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jeb/article/227/14/jeb247419/361243/Transgenesis-enables-mapping-of-segmental-gangliainfo:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.247419info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:05Zoai:ri.conicet.gov.ar:11336/261226instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:06.207CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
title Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
spellingShingle Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
Kuo, Dian Han
Sanguijuela
Neruonas
title_short Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
title_full Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
title_fullStr Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
title_full_unstemmed Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
title_sort Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
dc.creator.none.fl_str_mv Kuo, Dian Han
Szczupak, Lidia
Weisblat, David A.
Portiansky, Enrique Leo
Winchell, Christopher J.
Lee, Jun Ru
Tsai, Fu Yu
author Kuo, Dian Han
author_facet Kuo, Dian Han
Szczupak, Lidia
Weisblat, David A.
Portiansky, Enrique Leo
Winchell, Christopher J.
Lee, Jun Ru
Tsai, Fu Yu
author_role author
author2 Szczupak, Lidia
Weisblat, David A.
Portiansky, Enrique Leo
Winchell, Christopher J.
Lee, Jun Ru
Tsai, Fu Yu
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Sanguijuela
Neruonas
topic Sanguijuela
Neruonas
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The analysis of how neural circuits function in individuals and change during evolution is simplified by the existence of neurons identified as homologous within and across species. Invertebrates, including leeches, have been used for these purposes in part because their nervous systems comprise a high proportion of identified neurons, but technical limitations make it challenging to assess the full extent to which assumptions of stereotypy hold true. Here, we introduce Minos plasmid-mediated transgenesis as a tool for introducing transgenes into the embryos of the leech Helobdella austinensis (Spiralia; Lophotrochozoa; Annelida; Clitellata; Hirudinida; Glossiphoniidae). We identified an enhancer driving pan-neuronal expression of markers, including histone2B: mCherry, which allowed us to enumerate neurons in segmental ganglia. Unexpectedly, we found that the segmental ganglia of adult transgenic H. austinensis contain fewer and more variable numbers of neurons than in previously examined leech species.
Fil: Kuo, Dian Han. National Taiwan University; China
Fil: Szczupak, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Weisblat, David A.. University of California at Berkeley; Estados Unidos
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Winchell, Christopher J.. University of California at Berkeley; Estados Unidos
Fil: Lee, Jun Ru. National Taiwan University; China
Fil: Tsai, Fu Yu. National Taiwan University; China
description The analysis of how neural circuits function in individuals and change during evolution is simplified by the existence of neurons identified as homologous within and across species. Invertebrates, including leeches, have been used for these purposes in part because their nervous systems comprise a high proportion of identified neurons, but technical limitations make it challenging to assess the full extent to which assumptions of stereotypy hold true. Here, we introduce Minos plasmid-mediated transgenesis as a tool for introducing transgenes into the embryos of the leech Helobdella austinensis (Spiralia; Lophotrochozoa; Annelida; Clitellata; Hirudinida; Glossiphoniidae). We identified an enhancer driving pan-neuronal expression of markers, including histone2B: mCherry, which allowed us to enumerate neurons in segmental ganglia. Unexpectedly, we found that the segmental ganglia of adult transgenic H. austinensis contain fewer and more variable numbers of neurons than in previously examined leech species.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261226
Kuo, Dian Han; Szczupak, Lidia; Weisblat, David A.; Portiansky, Enrique Leo; Winchell, Christopher J.; et al.; Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis; Company of Biologists; Journal of Experimental Biology; 227; 14; 7-2024; 1-15
0022-0949
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261226
identifier_str_mv Kuo, Dian Han; Szczupak, Lidia; Weisblat, David A.; Portiansky, Enrique Leo; Winchell, Christopher J.; et al.; Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis; Company of Biologists; Journal of Experimental Biology; 227; 14; 7-2024; 1-15
0022-0949
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jeb/article/227/14/jeb247419/361243/Transgenesis-enables-mapping-of-segmental-ganglia
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.247419
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083269298225152
score 13.22299

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